Abstract
The availability of and easy access to Vitis genetic resources are essential for future breeding program advances. Cryopreservation is currently considered an ideal means for the long-term preservation of clonally propagated plant genetic resources. When robust methods for cryopreservation of Vitis spp. are available, there is an opportunity to preserve collections for extended lengths of time with minimal cost and labor requirements and a low risk of loss. This chapter describes the droplet vitrification and V cryo-plate protocols that have been shown to be effective for the cryopreservation of multiple V. vinifera genotypes and other Vitis species.
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References
Alleweldt G, Dettweiler E (1994) The genetic resources of Vitis: world list of grapevine collections, 2nd edn. Institut für Rebenzüchtung Geilweilerhof, Siebeldingen, Geilweilwehof
Benelli C, De Carlo A, Engelmann F (2013) Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnol Adv 31(2):175–185. https://doi.org/10.1016/j.biotechadv.2012.09.004
Benson EE (2008) Cryopreservation of phytodiversity: a critical appraisal of theory and practice. Crit Rev Plant Sci 27:141–219
Benson EE, Harding K (2012) Cryopreservation of shoot tips and meristems: an overview of contemporary methodologies. Methods Mol Biol 877:191–226. https://doi.org/10.1007/978-1-61779-818-4_16
Bettoni JC, Dalla Costa M, Gardin JPP, Kretzschmar AA, Pathirana R (2016) Cryotherapy: a new technique to obtain grapevine plants free of viruses. Rev Bras Frutic 38(2). https://doi.org/10.1590/0100-29452016833
Bettoni JC, Bonnart R, Shepherd AN, Kretzschmar AA, Volk GM (2019a) Cryopreservation of grapevine (Vitis spp.) shoot tips from growth chamber-sourced plants and histological observations. Vitis 58(2):71–78. https://doi.org/10.5073/vitis.2019.58.71-78
Bettoni JC, Bonnart R, Shepherd AN, Kretzschmar AA, Volk GM (2019b) Successful cryopreservation of Vitis vinifera ‘chardonnay’ from both in vitro and growth chamber source plants. Acta Hortic 1234:211–218. https://doi.org/10.17660/ActaHortic.2019.1234.28
Bettoni JC, Bonnart R, Shepherd AN, Kretzschmar AA, Volk GM (2019c) Modifications to a Vitis shoot tip cryopreservation procedure: effect of shoot tip size and use of cryoplates. CryoLetters 40(2):103–112
Bi W-L, Pan C, Hao X-Y, Cui Z-H, Kher MM, Marković Z, Wang Q-C, Teixeira da Silva JA (2017) Cryopreservation of grapevine (Vitis spp.)—a review. In Vitro Cell Dev Biol Plant 53(5):449–460. https://doi.org/10.1007/s11627-017-9822-9
Bi W-L, Hao X-Y, Cui Z-H, Volk GM, Wang Q-C (2018a) Droplet-vitrification cryopreservation of in vitro-grown shoot tips of grapevine (Vitis spp.). In Vitro Cell Dev Biol Plant 54:590–599. https://doi.org/10.1007/s11627-018-9931-0
Bi W-L, Hao X-Y, Cui Z-H, Pathirana R, Volk GM, Wang Q-C (2018b) Shoot tip cryotherapy for efficient eradication of Grapevine leafroll-associated virus-3 from diseased grapevine in vitro plants. Ann Appl Biol 173(3):261–270. https://doi.org/10.1111/aab.12459
Carimi F, Pathirana R, Carra A (2011) Biotechnologies for germplasm management and improvement. In: Szabo PV, Shojania J (eds) Grapevines – varieties, cultivation and management. Nova Science, New York, pp 199–249
Carimi F, Carra A, Panis B, Pathirana R (2016) Strategies for conservation of endangered wild grapevine (Vitis vinifera L. subsp. sylvestris (C.C. Gmel.) Hegi). Acta Hortic 1115:81–86. https://doi.org/10.17660/ActaHortic.2016.1115.13
Dussert S, Engelmann F, Noirot M (2003) Development of probabilistic tools to assist in the establishment and management of cryopreserved plant germplasm collections. CryoLetters 24(3):149–160
Eibach R, Zyprian E, Töpfer R (2009) The use of molecular markers for pyramidizing resistance genes in grapevine breeding. Acta Hortic 827:551–558. https://doi.org/10.17660/ActaHortic.2009.827.96
Markovic Z, Chatelet P, Sylvestre I, Kontic JK, Engelmann F (2013) Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips. Cent Eur J Biol 8(10):993–1000. https://doi.org/10.2478/s11535-013-0223-8
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Pathirana R, McLachlan A, Hedderley D, Panis B, Carimi F (2016) Pre-treatment with salicylic acid improves plant regeneration after cryopreservation of grapevine (Vitis spp.) by droplet vitrification. Acta Physiol Plant 38(1):1–11. https://doi.org/10.1007/s11738-015-2026-1
Reed BM (2014) Antioxidants and cryopreservation, the new normal? Acta Hortic 1039:41–48
Reed BM, Kovalchuk I, Kushnarenko S, Meier-Dinkel A, Schoenweiss K, Pluta S, Straczynska K, Benson EE (2004) Evaluation of critical points in technology transfer of cryopreservation protocols to international plant conservation laboratories. CryoLetters 25(5):341–352
Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9(1):30–33. https://doi.org/10.1007/BF00232130
Volk GM, Henk AD, Jenderek MM, Richards CM (2016) Probabilistic viability calculations for cryopreserving vegetatively propagated collections in genebanks. Genet Resour Crop Evol 64(7):1–10. https://doi.org/10.1007/s10722-016-0460-6
Volk GM, Shepherd AN, Bonnart R (2018) Successful cryopreservation of Vitis shoot tips: novel pre-treatment combinations applied to nine species. CryoLetters 39(5):322–330
Wang B, Wang R-R, Cui Z-H, Bi W-L, Li J-W, Li B-Q, Ozudogru EA, Volk GM, Wang Q-C (2014) Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication. Biotechnol Adv 32(3):583–595. https://doi.org/10.1016/j.biotechadv.2014.03.003
Wang M-R, Chen L, Teixeira da Silva JA, Volk GM, Wang Q-C (2018) Cryobiotechnology of apple (Malus spp.): development, progress and future prospects. Plant Cell Rep 37(5):689–709. https://doi.org/10.1007/s00299-018-2249-x
Yamamoto S, Rafique T, Priyantha WS, Fukui K, Matsumoto T, Niino T (2011) Development of a cryopreservation procedure using aluminium cryo-plates. CryoLetters 32(3):256–265
Acknowledgments
This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the author’s and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE. The authors are grateful to the ORISE/USDA for the Postdoctoral Researcher stipend granted to Jean Carlos Bettoni to perform research at the USDA-ARS National Laboratory for Genetic Resources Preservation (NLGRP). NLGRP Vitis cryopreservation research is funded in part by the Science and Technology Development Fund (Egypt). Ranjith Pathirana acknowledges the funding by Royal Society Te Apārangi for collaboration with USDA under the Catalyst Seeding project CSG-PAF1702.
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Bettoni, J.C., Pathirana, R., Bonnart, R., Shepherd, A., Volk, G. (2019). Cryopreservation of Grapevine Shoot Tips from In Vitro Plants Using Droplet Vitrification and V Cryo-plate Techniques. In: Faisal, M., Alatar, A. (eds) Synthetic Seeds . Springer, Cham. https://doi.org/10.1007/978-3-030-24631-0_22
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