Skip to main content
SpringerLink
Log in

In Vitro Conservation Through Slow-Growth Storage

  • Chapter
  • First Online:
Synthetic Seeds

Abstract

In vitro approaches are valuable for the conservation of plant biodiversity that includes the preservation of genetic resources of vegetatively propagated species, threatened plant species, taxa with recalcitrant seed, elite genotypes, and genetically modified/engineered material. The mid-term conservation is usually achieved by reducing the growth of in vitro cultures through the application of minimal media and growth retardant or storage at low temperatures resulting in prolonged intervals between the subcultures. Moreover, the combinations of all these factors are also employed for slow-growth storage. The medium-term conservation strategies are consistently employed for a large number of plant species, including various threatened species, from tropical as well as temperate origin. For long-term conservation of plant species, cryopreservation (storage in liquid nitrogen at −196 °C) is commonly employed. However, the main difficulties associated with cryopreservation are the maintenance of in vitro cultures as the procedure is highly technical and expensive since it involves a huge amount of resources and labor. In vitro slow-growth storage, therefore, enables a possible solution for mid- to long-term conservation of plant materials in limited space and at reduced costs too. Slow-growth procedures allow clonal plant conservation for several months to years (depending upon the species) under aseptic conditions, requiring the infrequent successive transfers of the cultures.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Acedo VZ, Arradaza CC (2012) Development of in vitro slow growth culture for Yam (Dioscorea alata L.). Ann Trop Res 34:79–95

    Article  Google Scholar 

  • Ahmed M, Anjum MA (2010) In vitro storage of some pear genotypes with the minimal growth technique. Turk J Agric For 34:25–32

    Google Scholar 

  • Akdemir H, Kaya E, Ozden Y (2010) In vitro proliferation and minimum growth storage of fraser photinia: influences of different medium, sugar combinations and culture vessels. Sci Hortic 126:268–275

    Article  Google Scholar 

  • Akdemir H, Süzerer V, Tilkat E, Yildirim H, Onay A, Çiftçi YO (2013) In vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm. J Plant Biochem Biotechnol 22:43–51

    Article  Google Scholar 

  • Assy-Bah B, Engelmann F (1993) Medium-term conservation of mature embryos of coconut. Plant Cell Tissue Organ Cult 33:19–24

    Article  CAS  Google Scholar 

  • Barba M, Gd L, Carimi F, Carra A, Abbate L, Chiota G (2008) Rescuing autochthonous grape vines thanks to virus elimination. Informatore Agrario Supplemento 64:14–16

    Google Scholar 

  • Barraco G, Sylvestre I, Iapichino G, Engelmann F (2013) Investigating the cryopreservation of nodal explants of Lithodora rosmarinifolia (Ten.) Johnst., a rare, endemic Mediterranean species. Plant Biotechnol Rep 7:141–146

    Article  Google Scholar 

  • Bekheet SA, Taha HS, Saker MM (2002) In vitro long-term storage of date palm. Biol Plant 45:121–124

    Article  Google Scholar 

  • Bessembinder JJE, Staritsky G, Zandvoort EA (1993) Long term in vitro storage of Colocasia esculenta under minimal growth conditions. Plant Cell Tissue Organ Cult 33:121–127

    Article  Google Scholar 

  • Bonnier FJM, van Tuyl JM (1997) Long term in vitro storage of lily: effects of temperature and concentration of nutrients and sucrose. Plant Cell Tissue Organ Cult 49:81–87

    Article  Google Scholar 

  • Budiarto K (2009) Alternative in vitro media for mid- term conservation of chrysanthemum. Jurnal Bumi Lestari 9:48–53

    Google Scholar 

  • Canto AMME, Souza FVD, Costa MAC, Souza AS, Ledo CAS, Cabral JRS (2004) In vitro conservation of pineapple germplasm treated with paclobutra- zol. Pesq Agrop Brasileira 36:717–720

    Article  Google Scholar 

  • Capuana M, Lonardo SD (2013) In vitro conservation of chestnut (Castanea sativa) by slow growth. In Vitro Cell Dev Biol Plant 49:605–610

    Article  CAS  Google Scholar 

  • Carimi F, Pathirana R, Carra A (2011) Biotechnologies for germplasm management and improvement. In: Szabo PV, Shojania J (eds) Grapevines-varieties, cultivation and management. Nova Science, Hauppauge, NY, pp 199–249

    Google Scholar 

  • Charoensub R, Phansiri S (2004) In vitro conservation of rose coloured leadwort: effect of mannitol on growth of plantlets. Kasetsart J (Nat Sci) 38:97–102

    CAS  Google Scholar 

  • Chauhan R, Keshavkant S, Jadhav SK, Quraishi A (2016) In vitro slow-growth storage of Chlorophytum borivilianum Sant. et Fernand: a critically endangered herb. In Vitro Cell Dev Biol Plant 52:315–321

    Article  CAS  Google Scholar 

  • Cha-um S, Kirdmanee C (2007) Minimal growth in vitro culture for preservation of plant species. Fruit Veg Cereal Sci Biotechnol 1:13–25

    Google Scholar 

  • Cordeiro SZ, Simas NK, Henriques AB, Sato A (2014) In vitro conservation of Mandevilla moricandiana (Apocynaceae): short-term storage and encapsulation-dehydration of nodal segments. In Vitro Cell Dev Biol Plant 50:326–336

    Article  CAS  Google Scholar 

  • Dhillon BS, Saxena S (2003) Conservation and access to plant genetic resources. In: Mandel BB, Chaudhury R, Engelmann F, Bhag M, Tao KL, Dhillon BS (eds) Conservation biotechnology of plant germplasm. NBPGR/IPGRI, FOA, New Delhi/Rome, pp 3–18

    Google Scholar 

  • Divakaran M, Babu KN, Peter KV (2006) Conservation of Vanilla species, in vitro. Sci Hortic 110:175–180

    Article  CAS  Google Scholar 

  • Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. Hortic Sci 19:507–509

    Google Scholar 

  • Dussert S, Chabrillange N, Anthony F, Engelmann F, Recalt C (1997) Variability in storage response with in a coffee (Coffea spp.) core collection under slow growth conditions. Plant Cell Rep 16:344–348

    CAS  PubMed  Google Scholar 

  • Engelmann F (1991) In vitro conservation of tropical plant germplasm – a review. Euphytica 57:227–243

    Article  Google Scholar 

  • Engelmann F (1992) Cryopreservation of embryos. In: Dattée Y, Dumas C, Gallais A (eds) Reproductive biology and plant breeding. Springer, Berlin, pp 281–290

    Chapter  Google Scholar 

  • Engelmann F (1998) In vitro conservation of horticultural genetic resources: review of the state of the art. World conference on horticultural research, Rome, pp 245–250

    Google Scholar 

  • Engelmann F (2004) Plant cryopreservation: progress and prospects. In Vitro Cell Dev Biol Plant 40:427–433

    Article  Google Scholar 

  • Engelmann F (2011) Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell Dev Biol Plant 47:5–16

    Article  Google Scholar 

  • Espindula MC, Rocha VS, Grossi JAS, Souza MA, Souza LT, Favarato LF (2009) Use of growth retardants in wheat. Planta Daninha 27:379–387

    Article  Google Scholar 

  • Filho AR, Dal Vesco LL, Nodari RO, Lischka RW, Muller CV, Guerra MP (2005) Tissue culture for the conservation and mass propagation of Vriesea reitzii Leme and Costa, a bromeliad threatened of extinction from the Brazilian Atlantic Forest. Biodivers Conserv 14:1799–1808

    Article  Google Scholar 

  • Fletcher PJ (1994) In vitro long-term storage of asparagus. N Z J Crop Hortic Sci 22:351–359

    Article  Google Scholar 

  • Gamborg OL, Miller RA, Ojima O (1968) Nutrient requirements of suspension cultures of soybean root cell. Exp Cell Res 50:151–158

    Article  CAS  PubMed  Google Scholar 

  • Gonçalves S, Romano A (2007) In vitro minimum growth for conservation of Drosophyllum lusitanicum. Biol Plant 51:795–798

    Article  Google Scholar 

  • Gopal J, Chauhan NS (2010) Slow growth in vitro conservation of potato germplasm at low temperature. Potato Res 53:141–149

    Article  Google Scholar 

  • Gopal J, Chamail A, Sarkar D (2002) Slow-growth in vitro conservation of potato germplasm at normal propagation temperature. Potato Res 45:203–213

    Article  Google Scholar 

  • Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 170:161–170

    Article  Google Scholar 

  • Grout BWW (1990) In vitro conservation of germplasm. In: Bhojwani SS (ed) Plant tissue culture: applications and limitations. Elsevier, Amsterdam, pp 394–423

    Chapter  Google Scholar 

  • Hansen J, Kristiansen K (1997) Short-term in vitro storage of Miscanthus ogiformis Honda “Giganteus” as affected by medium composition, temperature and photon flux density. Plant Cell Tissue Organ Cult 49:161–169

    Article  Google Scholar 

  • Hao Y-J, Deng X-X (2003) Genetically stable regeneration of apple plants from slow growth. Plant Cell Tiss Org Cult 72:253–260

    Article  CAS  Google Scholar 

  • Hasan NA, A El-Halwagi A, Gaber A, El-Awady M, Khalaf A (2007) Slow-growth in vitro conservation of Garlic cultivars grow in Egypt: chemical characterization and molecular evaluation. Global J Mol Sci 2:67–75

    Google Scholar 

  • Hassan NA, Bekheet SA (2008) Mid-term storage and genetic stability of strawberry tissue cultures. Res J Agric Biol Sci 4:505–511

    CAS  Google Scholar 

  • Holobiuc I, Blidu R, Carasan M, Helepciuc F, Voichita C, Negrean G (2008) In vitro conservation strategy in Veronica multifida ssp. capsellicarpa (dubovik) a. jelen. Rom Biol Plant Biol 53:71–81

    Google Scholar 

  • Islam MT, Leunufna S, Philibert Dembele D, Joachim Keller ER (2003) In vitro conservation of four Mint (Mentha spp.) accessions. Plant Tissue Org Cult 13:37–46

    Google Scholar 

  • Jarret RL, Gawel N (1991) Abscisic acid-induced growth inhibition of sweet potato (Ipomoea batatas L.) in vitro. Plant Cell Tissue Organ Cult 24:13–18

    Article  CAS  Google Scholar 

  • Joy RW, Kumar PP, Thorpe TA (1991) Long-term storage of somatic embryogenic White Spruce tissue at ambient temperature. Plant Cell Tissue Organ Cult 25:53–60

    Article  Google Scholar 

  • Kamińska M, Skrzypek E, Wilmowicz E, Tretyn A, Trejgell A (2016) Effect of light condition and ABA on cold storage and post-storage propagation of Taraxacum pieninicum. Plant Cell Tissue Organ Cult 127:25–34

    Article  CAS  Google Scholar 

  • Kamińska M, Tretyn A, Trejgell A (2018) Effect of jasmonic acid on cold-storage of Taraxacum pieninicum encapsulated shoot tips. Plant Cell Tissue Organ Cult. https://doi.org/10.1007/s11240-018-1481-y

    Article  CAS  Google Scholar 

  • Ko W, Hwang S, Ku F (1991) A new technique of meristem-tip cultures of ‘Cavendish’ banana. Plant Cell Tissue Organ Cult 25:179–183

    Google Scholar 

  • Koc I, Akdemir H, Onay A, Ozden Ciftci Y (2014) Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets. Acta Physiol Plant 36:2373–2384

    Article  CAS  Google Scholar 

  • Kovalchuk I, Lyudvikova Y, Volgina M, Reed BM (2009) Medium, container and genotype all influence in vitro cold storage of apple germplasm. Plant Cell Tissue Organ Cult 96:127–136

    Article  Google Scholar 

  • Kulkarni VM, Ganapathi TR (2009) A simple procedure for slow growth maintenance of banana (Musa spp.) embryogenic cell suspension cultures at low temperature. Curr Sci 96:1372–1377

    Google Scholar 

  • Lambardi M, Ozudogru EA (2013) Advances in the safe storage of micropropagated woody plants at low temperature. Acta Hortic (988):29–42

    Google Scholar 

  • Lata H, Moraes RM, Bertoni B, Pereira AMS (2010) In vitro germplasm conservation of Podophyllum peltatum L. under slow growth conditions. In Vitro Cell Dev Biol Plant 46:22–27

    Article  Google Scholar 

  • Li D, Pritchard HW (2009) The science and economics of ex situ plant conservation. Trends Plant Sci 14:614–621

    Article  CAS  PubMed  Google Scholar 

  • Linington SH (2003) The design of seed banks. In: Smith RD, Dickie JB, Linington SH, Pritchard HW, Probert RJ (eds) Seed conservation turning science into practice. Royal Botanic Gardens, Kew, pp 591–536

    Google Scholar 

  • Lopez-Delgado H, Jimenz-Casas M, Scott IM (1998) Storage of potato microplants in vitro in the presence of acetylsalicylic acid. Plant Cell Tissue Organ Cult 54:145–152

    Article  CAS  Google Scholar 

  • Lynch PT, Benson EE, Harding K (2007) Climate change: the role of economically important, vegetatively propagated plants. J Hortic Sci Biotechnol 82:157–160

    Article  Google Scholar 

  • Madubanya LA, Makunga NP, Fennell CW (2006) Dierama luteoalbidum: liquid culture provides an efficient system for the ex situ conservation of an endangered and horticulturally valuable plant. S Afr J Bot 72:584–588

    Article  Google Scholar 

  • Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: A tropical medicinal tree species. Sci Hortic 106:539–553

    Article  Google Scholar 

  • Marino G, Negri P, Cellini A, Masia A (2010) Effect of carbohydrates on in vitro low-temperature storage of shoot cultures of apricot. Sci Hortic 126:434–440

    Article  CAS  Google Scholar 

  • Martin KP, Pradeep AK (2003) Simple strategy for the in vitro conservation of Ipsea malabarica an endemic and endangered orchid of the Western Ghats of Kerala, India. Plant Cell Tissue Organ Cult 74:197–200

    Article  Google Scholar 

  • Maryam A, Tariq R, Chuadhary S, Azmat R, Javed S, Khanam S (2014) A review: role of tissue culture (in vitro) techniques in the conservation of rare and endangered species. Pac J Life Sci 2:93–103

    Google Scholar 

  • McCown BH, Lloyd G (1981) Woody Plant Medium (WPM) a mineral nutrient formulation for microculture for woody plant species. Hortic Sci 16:453

    Google Scholar 

  • Mitoi ME, Holobiuc I, Blindu R (2009) The effect of mannitol on antioxidative enzymes in vitro long term cultures of Dianthus tenuifolius and Dianthus spiculifolius. Rom J Biol Plant Biol 54:25–33

    Google Scholar 

  • Monette PL (1987) Organogenesis and plantlet regeneration following in vitro cold storage of kiwifruit shoot tip cultures. Sci Hortic 31:101–106

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murashige T, Tucker DPH (1969) Growth factor requirements of citrus tissue culture. In: Proceeding of 1st international citrus symposium, vol 3, pp 1155–1161

    Google Scholar 

  • Naidu MM, Sreenath HL (1999) In vitro culture of coffee zygotic embryo for germplasm preservation. Plant Cell Tissue Organ Cult 55:227–230

    Article  Google Scholar 

  • Nair DS, Reghunath BR, Soni KB, Alex S (2016) In vitro regeneration and conservation of Indigo (Indigofera tinctoria L.) by slow growth induction. Int J Environ Agric Biotechnol (4):873–884

    Google Scholar 

  • Nassar AH (2003) Slow growth storage of encapsulated germplasm of Coffea arabica L. Int J Agric Biol 5:517–520

    Google Scholar 

  • Negash A, Krens F, Schaart J, Visser B (2001) In vitro conservation of enset under slow-growth conditions. Plant Cell Tissue Organ Cult 66:107–111

    Article  CAS  Google Scholar 

  • Negri V, Tosti N, Standardi A (2000) Slow-growth storage of single node shoots of apple genotypes. Plant Cell Tissue Organ Cult 62:159–162

    Article  Google Scholar 

  • Niino T, Arizaga MV (2015) Cryopreservation for preservation of potato genetic resources. Breed Sci 65:41–52

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Novikova TI, Nabieva AY, Poluboyarova TV (2008) Conservation of rare and useful plants in in vitro collection of the Central Siberian Botanical Garden, Vestn. VOGi 12:564–571

    Google Scholar 

  • Ozden-Tokatli Y, Akdemir H, Tilkat E, Onay A (2010) Current status and conservation of Pistacia germplasm. Biotechnol Adv 28:130–141

    Article  CAS  PubMed  Google Scholar 

  • Ozudogru EA, Benelli C, Dradi G, Lambardi M (2017) Effect of culture container and carbohydrate content on in vitro slow growth storage of the cherry rootstock Gisela®5. Acta Physiol Plant 39:94

    Article  CAS  Google Scholar 

  • Pan XJ, Zhang W, Li X (2014) In vitro conservation of native Chinese wild grape (Vitis heyneana Roem. & Schult) by slow growth culture. Vitis 53:207–214

    CAS  Google Scholar 

  • Pathirana R, McLachlan A, Hedderley D, Panis B, Carimi F (2016) Pre-treatment with salicylic acid improves plant regeneration after cryopreservation of grapevine (Vitis spp.) by droplet vitrification. Acta Physiol Plant 38:12

    Article  CAS  Google Scholar 

  • Paunescu A (2009) Biotechnology for endangered plant conservation: a critical overview. Rom Biotechnol Lett 14:4095–4103

    CAS  Google Scholar 

  • Pedroso de Oliveira R, de Oliveira e Silva S, Magalhães da Silva K, Silveira DG (2000) In vitro conservation of diploid banana accessions. Sci Agric 57:245–249

    Article  Google Scholar 

  • Peng X, Ji Q, Wu H, Li Y (2015) Slow-growth conservation and clonal fidelity of Tetrastigma hemsleyanum microplants. In Vitro Cell Dev Biol Plant 51:463–470

    Article  CAS  Google Scholar 

  • Pérez-Molphe-Balch E, Pérez-Reyes ME, Rosa-Carrillo M (2012) In vitro conservation of Turbinicarpus (Cactaceae) under slow growth conditions. Haseltonia 17:51–57

    Article  Google Scholar 

  • Preil W, Hoffmann M (1985) In vitro storage in chrysanthemum breeding and propagation. In: Schafer-Menuhr A (ed) In vitro techniques – propagation and long term storage. Martinus Nijhoff/Junk, Dordrecht, pp 161–165

    Google Scholar 

  • Pruski K, Kozai T, Lewis T, Astatkie T, Nowak J (2000) Sucrose and light effects on in vitro cultures of potato, chokecherry and Saskatoon berry during low temperature. Plant Cell Tissue Organ Cult 63:215–221

    Article  CAS  Google Scholar 

  • Quoirin M, Lepoivre P (1977) Etude de milieux adaptés aux cultures in vitro de Prunus sp. Acta Hortic (78):437–442

    Google Scholar 

  • Quraishi A (2013) In vitro clonal propagation of forests trees: by bud culture technique. In: Behera KK (ed) Modern biotechnology and its applications, part I. New India Publishing Agency, New Delhi, pp 47–56

    Google Scholar 

  • Quraishi A, Mehar S, Sahu D, Jadhav SK (2017) In vitro mid-term conservation of Acorus calamus L. via cold storage of encapsulated microrhizome. Braz Arch Biol Technol 60:e17160378

    Article  CAS  Google Scholar 

  • Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants-a review. Biotechnol Adv 27:671–679

    Article  PubMed  Google Scholar 

  • Rao NK (2004) Plant genetic resources: advancing conservation and use through biotechnology: a review. Afr J Biotechnol 3:136–145

    Google Scholar 

  • Renau-Morata B, Arrillaga I, Segura J (2006) In vitro storage of cedar shoot cultures under minimal growth conditions. Plant Cell Rep 25:636–642

    Article  CAS  PubMed  Google Scholar 

  • Roberts HF (1973) Predicting the viability of seeds. Seed Sci Technol 1:499–514

    Google Scholar 

  • Roca WM, Reyes R, Brettran J (1984) Effect of various factors on minimal growth in tissue culture storage of cassava germplasm. In: 6th symposium of the international society for tropical root crops, Lima, pp 441–446

    Google Scholar 

  • Romano A, Martins-Loução MA (1999) In vitro storage of cork oak shoot cultures. Plant Cell Tissue Organ Cult 59:155–157

    Article  Google Scholar 

  • Rugini E (1984) In vitro propagation of some olive (Olea europaeasativar L.) cultivars with different root ability, and medium development using analytical data from developing shoots and embryos. Sci Hortic 24:123–134

    Article  CAS  Google Scholar 

  • Rugini E, Pesce PG (2006) Genetic improvement of olive. Pomol Croat 12:43–74

    Google Scholar 

  • Sajid GM, Ilyas MK, Anwar R (2006) Effect of diverse hormonal regimes on in vitro growth of grape germplasm. Pak J Bot 38:385–391

    Google Scholar 

  • Sarasan V (2010) Importance of in vitro technology to future conservation programmes worldwide. Kew Bull 65:549–554

    Article  Google Scholar 

  • Sarasan V, Cripps R, Ramsay MM, Atherton C, Mcmichen M, Prendergast G, Rowntree JK (2006) Conservation in vitro of threatened plants – progress in the past decades. In Vitro Cell Dev Biol Plant 42:206–214

    Article  Google Scholar 

  • Sarkar D, Naik PS (1998) Factors affecting minimal growth conservation of potato microplants in vitro. Euphytica 102:275–280

    Article  Google Scholar 

  • Sarkar D, Kaushik SK, Naik PS (1999) Minimal growth conservation of potato microplants: silver thiosulfate reduces ethylene-induced growth abnormalities during prolonged storage in vitro. Plant Cell Rep 18:897–903

    Article  CAS  Google Scholar 

  • Sarkar D, Chakrabarti SK, Naik PS (2001) Slow growth conservation of potato microplants: efficacy of ancymidol for long-term storage in vitro. Euphytica 117:133–142

    Article  CAS  Google Scholar 

  • Sarwar M, Siddiqui SU (2004) In vitro conservation of sugarcane (Saccharum officinarum L.) germplasm. Pak J Bot 36:549–556

    Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204

    Article  CAS  Google Scholar 

  • Scocchi AM, Mroginski LA (2004) In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow growth conditions. Int J Exp Bot 53:137–143

    Google Scholar 

  • Seitz U (1987) Cryopreservation of plant cell cultures. Planta Med 4:311–314

    Article  Google Scholar 

  • Sharma N, Satsangi R, Pandey R, Singh R, Kaushik N, Tyagi RK (2012) In vitro conservation of Bacopa monnieri (L.) using mineral oil. Plant Cell Tissue Organ Cult 111:291–301

    Article  CAS  Google Scholar 

  • Sharma SK, Patil A, Agnihotri AK, Mehrotra S (2018) In vitro conservation of Alectra chitrakutensis: a critically endangered root parasitic plant of high medicinal importance. Acta Physiol Plant 40:29

    Article  CAS  Google Scholar 

  • Shatnawi MA, Shibli RA, Abu-Romman SM, Al-Mazraawi MS, Ajlouni ZIA, Shatanawi WA, Odeh WH (2011) Clonal propagation and cryogenic storage of the medicinal plant Stevia rebaudiana. Span J Agric Res 9:213–220

    Article  Google Scholar 

  • Shibli RA, Shatnawi MA, Subaih WS, Ajlouni MM (2006) In vitro conservation and cryopreservation of plant genetic resources: a review. World J Agric Sci 2:372–382

    Google Scholar 

  • Silva RL, Ferreira CF, Ledo CAS, de Souza EH, da Silva PH, de Carvalho Costa MAP, Souza FVD (2016) Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation. Plant Cell Tissue Organ Cult 127:123–133

    Google Scholar 

  • Singh V, Koche V, Quraishi A (2018) Viral elimination strategies for Musa spp. Res Rev J Microbiol Virol 8:7–14

    CAS  Google Scholar 

  • Smith RH, Murashige T (1970) In vitro development of the isolated shoot apical meristem of angiosperms. Am J Bot 57:562–568

    Article  Google Scholar 

  • Son SH, Chun YW, Hall RB (1991) Cold storage of in vitro cultures of hybrid poplar shoots (Populus alba L. P. grandidentata Michx.). Plant Cell Tissue Organ Cult 27:161–168

    Article  Google Scholar 

  • Souza FVD, Cabral JRS, Almeida JV (2004) In vitro conservation of pineapple at embrapa cassava and fruits. Pineapple News 11:20–22

    Google Scholar 

  • Srivastava M, Purshottam DK, Srivastava AK, Misra P (2013) In vitro conservation of Glycyrrhiza glabra by slow growth culture. Int J Biotech Res 3:49–58

    Google Scholar 

  • Taylor PWJ, Dukic S (1993) Development of an in vitro culture technique for conservation of Saccharum spp. hybrid germplasm. Plant Cell Tissue Organ Cult 34:217–222

    Article  CAS  Google Scholar 

  • Thakur S, Tiwari KL, Jadhav SK (2015) In vitro approaches for conservation of Asparagus racemosus Willd. In Vitro Cell Dev Biol Plant 51:619–625

    Article  Google Scholar 

  • Trejgell A, Kaminska M, Tretyn A (2015) In vitro slow growth storage of Senecio macrophyllus shoots. Acta Physiol Plant 37:234

    Google Scholar 

  • Tyagi RK, Agrawal A, Mahalakshmi C, Hussain Z, Tyagi H (2007) Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers. In Vitro Cell Dev Biol Plant 43:51–58

    Article  CAS  Google Scholar 

  • Tyagi RK, Goswami R, Sanayaima R, Singh R, Tandon R, Agrawal A (2009) Micropropagation and slow growth conservation of cardamom (Elettaria cardamomum Maton). In Vitro Cell Dev Biol Plant 45:721–729

    Article  Google Scholar 

  • UNEP (2002) Global strategy for plant conservation, decision VI/9. Sixth ordinary meeting of the conference of the parties to the convention on biological diversity, The Hague, 7–9 April 2002. www.cbd.int/decision/cop/default.shtml?id=7180

  • Watt MP, Thokoane NL, Mycock D, Blakeway F (2000) In vitro storage of Eucalyptus grandis germplasm under minimal growth conditions. Plant Cell Tissue Organ Cult 61:161–164

    Google Scholar 

  • Watt MP, Banasiak M, Reddy D, Albertse EH, Snyman SJ (2009) In vitro minimal growth storage of Saccharum spp. hybrid (genotype 88H0019) at two stages of direct somatic embryogenic regeneration. Plant Cell Tissue Organ Cult 96:263–271

    Article  Google Scholar 

  • Williams RR, Taji AM (1987) Effects of temperature, darkness, and gelling agent on long-term storage of in vitro shoot cultures of Australian woody plant species. Plant Cell Tissue Organ Cult 11:151–156

    Article  Google Scholar 

  • Withers LA (1980) Preservation of Germplasm. In: Vasil I (ed) Perspectives in plant cell and tissue culture (international review of cytology, Suppl. 11B). Academic, New York, pp 101–133

    Google Scholar 

  • Withers LA (1991) Plant tissue culture: maintenance of plant tissue culture. In: Kirsop BE, Doyle A (eds) Maintenance of microorganism and cultured cells: a manual of laboratory methods, 2nd edn. Academic, San Diego, CA, pp 243–255

    Google Scholar 

  • Withers LA, Williams JT (1986) In vitro conservation. IBPGR, Rome, p 21

    Google Scholar 

  • Zandvoort EA, Staritsky G (1986) In vitro genebanks of tropical aroids – research of storage conditions. In: Somers DA et al (eds) Abstracts of the 6th international congress. Plant Tissue Cell Culture, Minneapolis, p 426

    Google Scholar 

  • Zandvoort EA, Hulshof MJH, Staritsky G (1994) In vitro storage of Xanthosoma spp. under minimal growth conditions. Plant Cell Tissue Organ Cult 36:309–316

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Center for Natural Resources, Pt. Ravishankar Shukla University, Raipur, India

    Ravishankar Chauhan

  2. School of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur, India

    Vikram Singh

  3. School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur, India

    Afaque Quraishi

Authors
  1. Ravishankar Chauhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vikram Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Afaque Quraishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Abdulrahman A. Alatar

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Chauhan, R., Singh, V., Quraishi, A. (2019). In Vitro Conservation Through Slow-Growth Storage. In: Faisal, M., Alatar, A. (eds) Synthetic Seeds . Springer, Cham. https://doi.org/10.1007/978-3-030-24631-0_19

Download citation

Publish with us

Policies and ethics

Search

Navigation