Abstract
Extremophiles are attracting a lot of focus by the researchers in the recent years and amongst them are a group of halophilic fungi inhabiting hypersaline environments ubiquitously distributed globally. In year 2000, the fungi were considered as halophiles which inhabit hypersaline habitats and are ubiquitously distributed throughout the world. However, most of the research on halophilic fungi has been focused on adaptation studies of these fungi in hypersaline habitats or as preliminary reports of biotechnological screenings where some of the applications have been well characterized and researched. However, in the recent years the research has diversified to topics like extremozymes, followed by bioremediation studies. The rapidly changing global environment, upcoming challenges in industrial biotechnology, and unique properties of the metabolites obtained from halophilic organisms and especially obligate halophilic fungi are promising to the prospective future of research in biotechnology.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ali I, Akbar A, Anwar M, Yanwisetpakdee B, Prasongsuk S, Lotrakul P, Punnapayak H (2014a) Purification and characterization of extracellular, polyextremophilic α-amylase obtained from halophilic Engyodontium album. Iran J Biotech 12:35–40. https://doi.org/10.15171/ijb.1155
Ali I, Akbar A, Yanwisetpakdee B, Prasongsuk S, Lotrakul P, Punnapayak H (2014b) Purification, characterization, and potential of saline waste water remediation of a polyextremophilic α-amylase from an obligate halophilic Aspergillus gracilis. Biomed Res Int 2014:7. https://doi.org/10.1155/2014/106937
Ali I, Siwarungson N, Punnapayak H, Lotrakul P, Prasongsuk S, Bankeeree W, Rakshit SK (2014c) Screening of potential biotechnological applications from obligate halophilic fungi, isolated from a man-made solar saltern located in Phetchaburi province. Thail Pak J Bot 46:983–988
Ali I, Akbar A, Anwar M, Prasongsuk S, Lotrakul P, Punnapayak H (2016) Purification and characterization of a polyextremophilic α-amylase from an obligate halophilic Aspergillus penicillioides isolate and its potential for souse with detergents. Biomed Res Int 2015:8. https://doi.org/10.1155/2015/245649
Annapurna SA, Singh A, Garg S, Kumar A, Kumar H (2012) Screening, isolation and characterisation of protease producing moderately halophilic microorganisms. Asian J Microbiol Biotechnol Environ Sci 14:603–612
Baati H, Guermazi S, Gharsallah N, Sghir A, Ammar E (2010) Microbial community of salt crystals processed from Mediterranean seawater based on 16S rRNA analysis. Can J Microbiol 56:44–51. https://doi.org/10.1139/W09-102
Bakke P, Carney N, Deloache W, Gearing M, Ingvorsen K, Lotz M, McNair J, Penumetcha P, Simpson S, Voss L, Win M, Heyer LJ, Campbell AM (2009) Evaluation of three automated genome annotations for Halorhabdus utahensis. PLoS One 4:6291. https://doi.org/10.1371/journal.pone.0006291
Bano A, Hussain J, Akbar A, Mehmood K, Anwar M, Hasni MS, Ullah S, Sajid S, Ali I (2018) Biosorption of heavy metals by obligate halophilic fungi. Chemosphere 199:218–222. https://doi.org/10.1016/j.chemosphere.2018.02.043
Berquist BR, Müller JA, DasSarma S (2006) 27 genetic systems for Halophilic Archaea. In: Methods in microbiology, vol 35. Academic Press, Cambridge, MA, pp 649–680. https://doi.org/10.1016/S0580-9517(08)70030-8
Butinar L, Sonjak S, Zalar P, Plemenitaš A, Gunde-Cimerman N (2005a) Melanized halophilic fungi are eukaryotic members of microbial communities in hypersaline waters of solar salterns. Bot Mar 48:73–79. https://doi.org/10.1515/BOT.2005.007
Butinar L, Zalar P, Frisvad JC, Gunde-Cimerman N (2005b) The genus Eurotium - members of indigenous fungal community in hyper-saline waters of salterns. FEMS Microbiol Ecol 51:155–166
DasSarma S, DasSarma P (2012) Halophiles. In eLS, (Ed.). doi:https://doi.org/10.1002/9780470015902.a0000394.pub3
De Hoog S, Zalar P, Van Den Ende BG, Gunde-Cimerman N (2005) Relation of halotolerance to human-pathogenicity in the fungal tree of life: an overview of ecology and evolution under stress. In: Adaptation to life at high salt concentrations in Archaea, Bacteria, and Eukarya. Springer, Netherlands, pp 371–395
Esawy MA, Awad GEA, Wahab WAA, Elnashar MMM, El-Diwany A, Easa SMH, El-beih FM (2016) Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads. 3 Biotech 6:29. https://doi.org/10.1007/s13205-015-0333-2
Geoffry K, Achur RN (2017) A novel halophilic extracellular lipase with both hydrolytic and synthetic activities. Biocat Agric Biotechnol 12:125–130. https://doi.org/10.1016/j.bcab.2017.09.012
Gostinčar C, Lenassi M, Gunde-Cimerman N, Plemenitaš A (2011) Chapter 3 - Fungal adaptation to extremely high salt concentrations. In: Laskin AI, Sariaslani S, Gadd GM (eds) Advances in applied microbiology, vol 77. Academic Press, Cambridge, MA, pp 71–96. https://doi.org/10.1016/B978-0-12-387044-5.00003-0
Gunde-Cimerman N, Zalar P, de Hoog S, Plemenitaš A (2000) Hypersaline waters in salterns: natural ecological niches for halophilic black yeasts. FEMS Microbiol Ecol 32:235–240
Gunde-Cimerman N, Zalar P, Petrovič U, Turk M, Kogej T, de Hoog GS, Plemenitaš A (2004) Fungi in salterns. In: Ventosa A (ed) Halophilic microorganisms. Springer, Berlin, pp 103–113. https://doi.org/10.1007/978-3-662-07656-9_7
Gunde-Cimerman N, Ramos J, Plemenitaš A (2009) Halotolerant and halophilic fungi. Mycol Res 113:1231–1241. https://doi.org/10.1016/j.mycres.2009.09.002
Gunny AAN, Arbain D, Edwin Gumba R, Jong BC, Jamal P (2014) Potential halophilic cellulases for in situ enzymatic saccharification of ionic liquids pretreated lignocelluloses. Bioresour Technol 155:177–181. https://doi.org/10.1016/j.biortech.2013.12.101
Gunny AAN, Arbain D, Jamal P, Gumba RE (2015) Improvement of halophilic cellulase production from locally isolated fungal strain. Saudi J Biol Sci 22:476–483. https://doi.org/10.1016/j.sjbs.2014.11.021
Jančič S, Frisvad JC, Kocev D, Gostinčar C, Džeroski S, Gunde-Cimerman N (2016) Production of secondary metabolites in extreme environments: food and airborne Wallemia spp. produce toxic metabolites at hypersaline conditions. PLoS One 11(12):e0169116. https://doi.org/10.1371/journal.pone.0169116
Jiang Y, Yang K, Wang H, Shang Y, Yang X (2015) Characteristics of phenol degradation in saline conditions of a halophilic strain JS3 isolated from industrial activated sludge. Mar Pollut Bull 99:230–234. https://doi.org/10.1016/j.marpolbul.2015.07.021
Jiang Y, Shang Y, Yang K, Wang H (2016) Phenol degradation by halophilic fungal isolate JS4 and evaluation of its tolerance of heavy metals. Appl Microbiol Biotechnol 100:1883–1890. https://doi.org/10.1007/s00253-015-7180-2
Khan MN, Lin H, Li M, Wang J, Mirani ZA, Khan SI, Buzdar MA, Ali I, Jamil K (2017) Identification and growth optimization of a Marine Bacillus DK1-SA11 having potential of producing broad spectrum antimicrobial compounds. Pak J Pharm Sci 30:839–853
Kis-Papo T et al (2014) Genomic adaptations of the halophilic Dead Sea filamentous fungus Eurotium rubrum. Nat Commun 5:3745. https://doi.org/10.1038/ncomms4745
Lenassi M et al (2013) Whole genome duplication and enrichment of metal cation transporters revealed by De Novo Genome Sequencing of extremely halotolerant black yeast Hortaea werneckii. PLoS One 8:e71328. https://doi.org/10.1371/journal.pone.0071328
Lenka J, Maharana AK, Priyadarshini S, Mohanty D, Ray P (2016) Isolation and characterization of marine micro-flora from coast of Paradip and screening for potent enzymes. RJPBCS 7:1187–1196
Liu KH, Ding SW, Narsing RMP, Zhang B, Zhang YG, Liu FH, Liu BB, Xio M, Li WJ (2017) Morphological and transcriptomic analysis reveals the osmoadaptive response of endophytic fungus Aspergillus montevidensis ZYD4 to high salt stress. Front Microbiol 8:1789. https://doi.org/10.3389/fmicb.2017.01789
Mansour MMA (2017) Effects of the halophilic fungi Cladosporium sphaerospermum, Wallemia sebi, Aureobasidium pullulans and Aspergillus nidulans on halite formed on sandstone surface. Int Biodeter Biodegr 117:289–298. https://doi.org/10.1016/j.ibiod.2017.01.016
Miyashita Y, Ohmae E, Ikura T, Nakasone K, Katayanagi K (2017) Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1. Extremophiles 21:591–602. https://doi.org/10.1007/s00792-017-0928-0
Oren A (2008) Microbial life at high salt concentrations: phylogenetic and metabolic diversity. Saline Sys 4:2. https://doi.org/10.1186/1746-1448-4-2
Oren A (2010) Industrial and environmental applications of halophilic microorganisms. Environ Technol 31:825–834. https://doi.org/10.1080/09593330903370026
Oren A (2013) Life at high salt concentrations. In: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (eds) The prokaryotes: prokaryotic communities and ecophysiology. Springer, Berlin, Heidelberg, pp 421–440. https://doi.org/10.1007/978-3-642-30123-0_57
Paul S, Bag SK, Das S, Harvill ET, Dutta C (2008) Molecular signature of hypersaline adaptation: insights from genome and proteome composition of halophilic prokaryotes. Genome Biol 9:R70. https://doi.org/10.1186/gb-2008-9-4-r70
Plemenitaš A, Gunde-Cimerman N (2005) Cellular responses in the halophilic black yeast Hortaea Werneckii to high environmental salinity. In: Adaptation to life at high salt concentrations in Archaea, Bacteria, and Eukarya. Springer, Netherlands, pp 453–470
Plemenitaš A, Vaupotič T, Lenassi M, Kogej T, Gunde-Cimerman N (2008) Adaptation of extremely halotolerant black yeast Hortaea werneckii to increased osmolarity: a molecular perspective at a glance. Stud Mycol 61:67–75. https://doi.org/10.3114/sim.2008.61.06
Ravindran C, Varatharajan GR, Rajasabapathy R, Vijayakanth S, Kumar AH, Meena RM (2012) A role for antioxidants in acclimation of marine derived pathogenic fungus (NIOCC 1) to salt stress. Microb Pathog 53:168–179. https://doi.org/10.1016/j.micpath.2012.07.004
Sarwar KM, Azam I, Iqbal T (2015) Biology and applications of halophilic bacteria and Archaea: a review. Electron J Biol 11:98–103
Schafer G, Engelhard M, Muller V (1999) Bioenergetics of the Archaea. Microbiol Mol Biol Rev 63:570–620
Sepcic K, Zalar P, Gunde-Cimerman N (2011) Low water activity induces the production of bioactive metabolites in halophilic and halotolerant fungi. Mar Drugs 9:43
Sinha SK, Goswami S, Das S, Datta S (2017) Exploiting non-conserved residues to improve activity and stability of Halothermothrix orenii β-glucosidase. Appl Microbiol Biotechnol 101:1455–1463. https://doi.org/10.1007/s00253-016-7904-y
Talon R, Coquelle N, Madern D, Girard E (2014) An experimental point of view on hydration/solvation in halophilic proteins. Front Microbiol 5:66. https://doi.org/10.3389/fmicb.2014.00066
Tiquia SM (2010) Salt-adapted bacteria isolated from the Rouge River and potential for degradation of contaminants and biotechnological applications. Environ Technol 31:967–978
Tiquia SM, Mormile M (2010) Extremophiles–a source of innovation for industrial and environmental applications. Environ Technol 31(8–9):823
Tiquia SM, Davis D, Hadid H, Kasparian S, Ismail M, Sahly R, Shim J, Singh S, Murray KS (2007) Halophilic and halotolerant bacteria from river waters and shallow groundwater along the Rouge River of Southeastern Michigan. Environ Technol 28:297–307
Tiquia-Arashiro SM, Rodrigues D (2016a) Extremophiles: applications in nanotechnology. Springerbriefs in microbiology: extremophilic microorganisms. Springer International Publishing, New York, p 193
Tiquia-Arashiro SM, Rodrigues D (2016b) Halophiles in nanotechnology. In: Extremophiles: applications in nanotechnology. Springer International Publishing, New York, pp 53–58
Xiao L, Liu H, Wu N, Liu M, Wei J, Zhang Y, Lin X (2013) Characterization of the high cytochalasin E and rosellichalasin producing Aspergillus sp. nov. F1 isolated from marine solar saltern in China. World J Microbiol Biotechnol 29:11–17. https://doi.org/10.1007/s11274-012-1152-9
Zajc J, Liu Y, Dai W, Yang Z, Hu J, Gostinčar C, Gunde-Cimerman N (2013) Genome and transcriptome sequencing of the halophilic fungus Wallemia ichthyophaga: haloadaptations present and absent. BMC Genomics 14:617. https://doi.org/10.1186/1471-2164-14-617
Zambelli P, Serra I, Arrojo LF, Plou FJ, Tamborini L, Conti P, Contente ML, Molinari F, Romano D (2015) Sweet-and-salty biocatalysis: fructooligosaccharides production using Cladosporium cladosporioides in seawater. Process Biochem 50:1086–1090. https://doi.org/10.1016/j.procbio.2015.04.006
Zusman T, Rosenshine I, Boehm G, Jaenicke R, Leskiw B, Mevarech M (1989) Dihydrofolate reductase of the extremely halophilic archaebacterium Halobacterium volcanii. J Biol Chem 264:18878–18883
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Ali, I., Khaliq, S., Sajid, S., Akbar, A. (2019). Biotechnological Applications of Halophilic Fungi: Past, Present, and Future. In: Tiquia-Arashiro, S., Grube, M. (eds) Fungi in Extreme Environments: Ecological Role and Biotechnological Significance. Springer, Cham. https://doi.org/10.1007/978-3-030-19030-9_15
Download citation
DOI: https://doi.org/10.1007/978-3-030-19030-9_15
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-19029-3
Online ISBN: 978-3-030-19030-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)