Abstract
Despite of silver nanopaticles’ (AgNPs) widespread use, there is a serious lack of information concerning the toxicity of AgNPs to humans and their key cellular actions. The aim of the present study was an effect of different concentrations of 40 nm AgNPs and time of incubation on the viability, CD 44 expression, mitochondrial state, and apoptotic/necrotic processes in bone marrow-derived mesenchymal stem cells (MSCs). The obtained results suggested that 1-hour incubation with AgNPs in the concentrations of 2 and 4 μg/ml did not affect the viability and content of CD44-positive cells, as well as did not cause the development of necrosis/apoptosis processes and alteration of mitochondrial activity in MSCs. After AgNPs’ addition in the concentrations of 6 and 10 μg/ml there were a decrease of mitochondrial activity, percentage of viability cells and an increase of the number of apoptotic cells compared with the control samples of MSCs. An increase in the period of incubation with AgNPs (4–10 μg/ml) up to 1 day leads to a toxic effect on MSCs, which was manifested in the decrease of viability, content of CD44-positive cells (AgNPs 10 μg/ml), mitochondrial activity, and activation of apoptosis and necrosis. The obtained results are related to the field of applied nanotechnology, which extends to clinical medicine, especially in the development of addressed drug delivery to the target cells or organs.
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References
Henig RM (2007) Our silver-coated future. Onearth 29(3):22–29
Lara HH, Garza-Treviсo EN, Ixtepan-Turrent L, Singh DK (2011) Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotechnol 9(3):30–39
You C, Han C, Wang X et al (2012) The progress of silver nanoparticles in the antibacterial mechanism, clinical application and cytotoxicity. Mol Biol Rep 39(9):9193–9201
Faunce T, Watal A (2010) Nanosilver and global public health: international regulatory issues. Nanomedicine 5(4):617–632
Lima R, Feitosa LO, Ballottin D (2013) Cytotoxicity and genotoxicity of biogenic silver nanoparticles. J Phys Conf Ser 429(2):8–11
Carlson C, Hussain SM, Schrand AM et al (2008) Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B 112(6):13608–13619
Park MV, Neigh AM, Vermeulen JP et al (2011) The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. Biomaterials 32(1):9810–9817
Greulich C, Kittler S, Epple M et al (2009) Studies on the biocompatibility and the interaction of silver nanoparticles with human mesenchymal stem cells (hMSCs). Langenbeck’s Arch Surg 394(3):495–502
Greulich C, Braun D, Peetsch A et al (2012) The toxic effect of silver ions and silver nanoparticles towards bacteria and human cells occurs in the same concentration range. RSC Adv 2(17):6981–6987
Kittler S, Greulich C, Gebauer JS et al (2009) Synthesis of PVP-coated silver nanoparticles and their biological activity towards human mesenchymal stem cells. Mater Chem 40(4):258–264
Kittler S, Greulich C, Diendorf J et al (2010) Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions. Chem Mater 22(16):4548–4554
Loza K, Diendorf J, Greulich C et al (2014) The dissolution and biological effects of silver nanoparticles in biological media. J Mater Chem 2(12):1634–1643
Pittenger MF, Mackay AM, Beck SC et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284(5411):143–147
Baksh D, Song L, Tuan RS (2004) Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 8(3):301–316
Greulich C, Diendorf J, Simon T et al (2011) Uptake and intracellular distribution of silver nanoparticles in human mesenchymal stem cells. Acta Biomater 7(1):347–354
De Lima R, Seabra AB, Durn N (2012) Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles. J Appl Toxicol 32(4):867–879
Zhang T, Wang L, Chen Q, Chen C (2014) Cytotoxic potential of silver nanoparticles. Yonsei Med J 55(2):283–291
Volkova N, Pavlovich O, Fesenko O et al (2017) Studies of the influence of gold nanoparticles on characteristics of mesenchymal stem cells. J Nanomater 2017:6934757
Norkus M, Kilmartin L, Fay D et al (2013) The effect of temperature elevation on cryopreserved mesenchymal stem cells. CryoLetters 34(4):349–359
Soenen SJ, Manshian B, Montenegro JM et al (2012) Cytotoxic effects of gold nanoparticles: a multiparametric study. ACS Nano 6:5767–5783
Hackenberg S, Scherzed A, Kessler M et al (2011) Silver nanoparticles: evaluation of DNA damage, toxicity and functional impairment in human mesenchymal stem cells. Toxicol Lett 201:27–33
Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapу 8(4):315–317
Kim K, Lee M, Park H (2006) Cell-permeable and biocompatible polymeric nanoparticles for apoptosis imaging. J Am Chem Soc 22(11):3490–3491
Rim K-T, Song S-W, Kim H-Y (2013) Oxidative DNA damage from nanoparticle exposure and its application to workers’ health: a literature review. Saf Health Work 4(4):177–186
Pavlovich EV, Volkova NA (2015) Influence of gold nanoparticles on human fibroblast before and after cryopreservation. In: Fesenko O, Yatsenko L (eds) Nanoplasmonics, nano-optics, nanocomposites, and surface studies, Springer proceedings in physics 156. Springer, Cham
Cossarizza A, Baccaranicontri M, Kalashnikova G et al (1993) A new method for the cytofluorometric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem Biophys Res Commun 197(1):40–45
Bressan E, Ferroni L, Gardin C et al (2013) Silver nanoparticles and mitochondrial interaction. Int J Dent 2013:312747
AshaRani PV, Low Kah Mun G, Hande MP et al (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3:279–290
Acknowledgments
This work has been supported by project No 70/18-Н NAS of Ukraine “Biosafety and efficiency of interaction of inert metal nanoparticles with mesenchymal stem cells of bone marrow.”
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Volkova, N.A., Yukhta, M.S., Pavlovich, E.V., Goltsev, A.N. (2019). Change in Functional State of Bone Marrow-Derived Mesenchymal Stem Cells After Incubation with Silver Nanoparticles. In: Fesenko, O., Yatsenko, L. (eds) Nanophotonics, Nanooptics, Nanobiotechnology, and Their Applications. NANO 2018. Springer Proceedings in Physics, vol 222. Springer, Cham. https://doi.org/10.1007/978-3-030-17755-3_19
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