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Contemporary Acarology pp 79–91Cite as

Mycobiome of Brevipalpus Mite Strains and Insights on Metabolic Function in the Bacteriome of the Tetranychoidea Mites

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Abstract

Studies of arthropod microbiota and arthropod-microbe interactions are helping elucidate the strategies adopted by arthropods to colonize and succeed in complex environments, as well as leading to the development of unique pest management approaches. Tetranychoidea (Acariformes: Trombidiformes) are important pests of several crops due to their feeding habits and transmission of pathogens. In tetranychoid mites, the endosymbiont bacterium Cardinium represents a tantalizing first target for pest management research because it affects mite reproduction. In this study, we used previously published 16S ribosomal RNA sequence data of the microbiome bacteria in Brevipalpus yothersi (Baker 1949), Raoiella indica Hirst, 1924, and Oligonychus sp. and the PICRUSt pipeline to predict the content of genes with metabolic function in the bacteriome of the mites. Our results indicate that the bacteriomes of B. yothersi and Oligonychus sp. (which harbor Cardinium) contain significantly more genes involved in the metabolism of indole-alkaloids, glutamine, and biotin when compared with R. indica (which has no Cardinium). The genes for metabolism of biotin and nicotinate are also more abundant in adult B. yothersi and Oligonychus sp. than in their eggs, which is associated with lower abundance of Cardinium in the eggs. The metabolic specialization of Cardinium-dominated bacteriomes could also lead to lack of resistance to β-lactam antibiotics and DDT. While these results are predictive, they highlight the necessity of testing these variations in laboratory. We also present initial data on fungal microbial diversity associated with four different strains of the phytophagous mite vector Brevipalpus, which showed significant variation between strains, while all are dominated by the skin- and surface-specialist genus Malassezia.

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Acknowledgements

To USDA/APHIS 8130-0059-CA and USDA-Hatch 427 for partial financing of this work and NIFA-06242 grant for infrastructural improvement.

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Authors and Affiliations

  1. Agro-Environmental Sciences Department, Center for Excellence in Quarantine & Invasive Species, Agricultural Experimental Station - Río Piedras, University of Puerto Rico, 1193, Calle Guayacan, San Juan, PR, 00926, USA

    Jose Carlos Verle Rodrigues & Oscar E. Ospina

  2. Bioinformatics Laboratory, Department of Biology, University of Puerto Rico – Rio Piedras, San Juan, PR, 00931-3360, USA

    Steven E. Massey

Authors
  1. Jose Carlos Verle Rodrigues
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  2. Oscar E. Ospina
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  3. Steven E. Massey
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Corresponding author

Correspondence to Jose Carlos Verle Rodrigues .

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Editors and Affiliations

  1. Department of Entomology, Pennsylvania State University, Agricultural Science & Industries Building, University Park, PA, USA

    Michael J. Skvarla

  2. Systematic Entomology Laboratory, USDA Agricultural Research Service, Beltsville, MD, USA

    Ronald Ochoa

  3. Jardin Botanico Sur, University of Puerto Rico EEA, San Juan, Puerto Rico

    Jose Carlos Verle Rodrigues

  4. National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada

    H. Joel Hutcheson

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Rodrigues, J.C.V., Ospina, O.E., Massey, S.E. (2019). Mycobiome of Brevipalpus Mite Strains and Insights on Metabolic Function in the Bacteriome of the Tetranychoidea Mites. In: Skvarla, M., Ochoa, R., Verle Rodrigues, J., Hutcheson, H. (eds) Contemporary Acarology . Springer, Cham. https://doi.org/10.1007/978-3-030-17265-7_5

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