Abstract
Rodent transient middle cerebral artery occlusion (MCAO) is one of the widely used focal ischemia models in the world. This model mimics human cerebral ischemic injury and provides a unique tool for the mechanistic studies of ischemia in brain tissues in vivo. Rodent ischemia and reperfusion models are commonly used because of a greater understanding of rodent genetics, the availability of specific antibodies to rodent epitopes and molecular probes in rodents, and the ability to study transgenic mouse strains. In this chapter, we discuss: (1) the preparation of MCAO in rodents; (2) the intra-luminal MCAO techniques and its modifications; (3) the quantitative evaluation of model success; and (4) the advantages and limitations of MCAO models.
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Mao, Y., Zhu, W., Yang, GY. (2019). Transient Middle Cerebral Artery Occlusion Model in Rodents. In: Chen, J., Xu, Z., Xu, X., Zhang, J. (eds) Animal Models of Acute Neurological Injury. Springer Series in Translational Stroke Research. Springer, Cham. https://doi.org/10.1007/978-3-030-16082-1_9
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DOI: https://doi.org/10.1007/978-3-030-16082-1_9
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