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Unravelling the Soil Microbiome pp 33–49Cite as

Single-Cell Genomics and Metagenomics for Microbial Diversity Analysis

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Part of the book series: SpringerBriefs in Environmental Science ((BRIEFSENVIRONMENTAL))

Abstract

Soil metagenomic analysis was previously limited by technological restrictions and the few reference genomes. The advent of next-generation ‘omics’ technologies has provided high-throughput methods for analysing community structure and reconstructing soil metagenomes. High-throughput sequencing technology and single-cell genomics have revolutionized metagenomic analysis by enabling large-scale sequencing at reduced sequencing costs with less time required. In the present chapter we discuss various technological advances in metagenomics, their processes and the methods of data analysis, and metagenomic success stories under various environments that can be applied for studying the functional and structural diversity of soil microorganisms.

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Authors and Affiliations

  1. Institute of Environment & Sustainable Development, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Rama Kant Dubey, Vishal Tripathi, Rajan Chaurasia & Purushothaman Chirakkuzhyil Abhilash

  2. Chhattisgarh Swami Vivekananda Technical University, Bhilai, Chhattisgarh, India

    Ratna Prabha

  3. ICAR-National Bureau of Agriculturally Important Microorganisms, Mau Nath Bhanjan, Uttar Pradesh, India

    Dhananjaya Pratap Singh

  4. National Academy of Agricultural Research Management, Hyderabad, Telangana, India

    Ch. Srinivasa Rao

  5. Department of Applied Biology, University of Sharjah, Sharjah, United Arab Emirates

    Ali El-Keblawy

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Dubey, R.K. et al. (2020). Single-Cell Genomics and Metagenomics for Microbial Diversity Analysis. In: Unravelling the Soil Microbiome. SpringerBriefs in Environmental Science. Springer, Cham. https://doi.org/10.1007/978-3-030-15516-2_4

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