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Ion Exchange Chromatography for Enzyme Immobilization

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Applications of Ion Exchange Materials in Biomedical Industries

Abstract

The use of ion-exchange materials for separation and purification of proteins is a widely studied and applied tool since the twentieth century. Following the same basic principle of charge interactions, new applications for ion-exchange materials have emerged in recent decades. Enzymatic immobilization technology is one of the most promising applications in terms of bioconversion processes. The main current demands of the biotechnology industries that use enzymatic catalysis in conversion processes are the increase in productivity and reduction of overall costs. These needs can be met mainly by optimizing the enzymatic properties provided by enzyme immobilization in several carrier/materials. Enzymatic immobilization in ion-exchange materials is exceptionally simpler when compared to other immobilization methods. It basically involves electrostatic/ionic interactions of weak nature between protein and resin. These weak interactions generate minimal conformational changes, improving enzyme chemical and physical stabilities, and increases specificity, enzyme selectivity, and catalytic activities. All these advantages make this application more attractive to industry. However, much research and incentives are still needed to make this technology more robust, efficient and widespread in several industrial sectors. This chapter pays particular attention to ion-exchange chromatography as a robust tool to improve enzyme immobilization.

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Authors and Affiliations

  1. Department of Cell Biology, University of Brasilia, 70910-900, Brasília, Distrito Federal, Brazil

    Raissa Pieroni Vaz & Edivaldo Ximenes Ferreira Filho

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  1. Raissa Pieroni Vaz
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  2. Edivaldo Ximenes Ferreira Filho
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Correspondence to Edivaldo Ximenes Ferreira Filho .

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  1. Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Inamuddin

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Vaz, R.P., Filho, E.X.F. (2019). Ion Exchange Chromatography for Enzyme Immobilization. In: Inamuddin (eds) Applications of Ion Exchange Materials in Biomedical Industries. Springer, Cham. https://doi.org/10.1007/978-3-030-06082-4_2

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