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Electrochemical DNA-Based Sensor for Organophosphorus Pesticides Detection

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Sensors (CNS 2018)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 539))

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Abstract

In this work, we propose an electrochemical DNA-based sensor for sensitive detection of organophosphorus pesticides. To improve the sensitivity of the DNA-based sensor, polyaniline film and gold nanoparticles were progressively electrodeposited on the graphite screen-printed electrode surface by cyclic voltammetry. Gold nanoparticles were then employed as platform for the immobilization of thiol-tethered DNA oligonucleotide sequence complementary to the selected biotinylated DNA aptamer for profenofos detection. Streptavidin-alkaline phosphatase enzyme conjugate was then added to trace the affinity reaction through the hydrolysis of 1-naphthyl phosphate to 1-naphthol, which was then detected by differential pulse voltammetry. A decrease of the signal was obtained when the pesticide concentration was increased, making the sensor work as signal off sensor.

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Authors and Affiliations

  1. University of Florence, Via della Lastruccia 3-13, 50019, Sesto Fiorentino, Italy

    Giulia Selvolini, Oana Hosu & Giovanna Marrazza

  2. University of Medicine and Pharmacy “Iuliu Hatieganu”, 4 Louis Pasteur Street, 400439, Cluj Napoca, Romania

    Ioana Băjan, Oana Hosu, Cecilia Cristea & Robert Săndulescu

Authors
  1. Giulia Selvolini
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  2. Ioana Băjan
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  3. Oana Hosu
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  4. Cecilia Cristea
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  5. Robert Săndulescu
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  6. Giovanna Marrazza
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Corresponding author

Correspondence to Giovanna Marrazza .

Editor information

Editors and Affiliations

  1. University of Catania, Catania, Italy

    Bruno Andò

  2. IFAC-CNR, Sesto Fiorentino, Florence, Italy

    Francesco Baldini

  3. University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. Department of Information Engineering (DII), University of Brescia, Brescia, Italy

    Vittorio Ferrari

  5. University of Catania, Catania, Italy

    Vincenzo Marletta

  6. Department of Chemistry, University of Florence, Sesto Fiorentino, Florence, Italy

    Giovanna Marrazza

  7. University of Palermo, Palermo, Italy

    Valeria Militello

  8. University of Padova, Padua, Italy

    Giorgia Miolo

  9. Sapienza University of Rome, Rome, Italy

    Marco Rossi

  10. Università Politecnica delle Marche (UNIVPM), Ancona, Italy

    Lorenzo Scalise

  11. IMM-CNR, Lecce, Italy

    Pietro Siciliano

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Selvolini, G., Băjan, I., Hosu, O., Cristea, C., Săndulescu, R., Marrazza, G. (2019). Electrochemical DNA-Based Sensor for Organophosphorus Pesticides Detection. In: Andò, B., et al. Sensors. CNS 2018. Lecture Notes in Electrical Engineering, vol 539. Springer, Cham. https://doi.org/10.1007/978-3-030-04324-7_15

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  • DOI: https://doi.org/10.1007/978-3-030-04324-7_15

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-04323-0

  • Online ISBN: 978-3-030-04324-7

  • eBook Packages: EngineeringEngineering (R0)

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