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Fluorescence Microscopy

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Springer Handbook of Microscopy

Part of the book series: Springer Handbooks ((SHB))

Abstract

In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup. This is followed by a critical account of the many super-resolution techniques: coordinated stochastic fluorescence microscopy (photoactivation localization microscopy ( ), stochastic optical reconstruction microscopy ( ), point accumulation for imaging in nanoscale topography ( ), coordinate targeted fluorescence microscopy (STED, reversible saturable optical fluorescence transition ( )), structured illumination microscopy, expansion microscopy ( ), and liquid tunable microscopy ( ).

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Acknowledgements

The first Italian TPE architecture realized at LAMBS was supported by INFM grants. LAMBS-MicroScoBio is currently funded by IFOM (Istituto FIRC di Oncologia Molecolare, FIRC Institute of Molecular Oncology, Milano). This chapter is dedicated to the memory of Osamu Nakamura, who passed away January 23, 2005 at Handai Hospital.

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Authors and Affiliations

  1. Dept. of Nanophysics, Italian Institute of Technology, Genoa, Italy

    Alberto Diaspro, Francesca Cella Zanacchi, Luca Lanzanò & Luca Pesce

  2. Dept. of Nanoscopy, Italian Institute of Technology, Genoa, Italy

    Paolo Bianchini & Giuseppe Vicidomini

  3. Nikon Imaging Center, Italian Institute of Technology, Genoa, Italy

    Michele Oneto

  4. Dept. of Physics, University of Genoa, Genoa, Italy

    Isotta Cainero

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  1. Alberto Diaspro
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Correspondence to Alberto Diaspro .

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  1. CEMES-CNRS, Toulouse, France

    Peter W. Hawkes

  2. Dept. of Physics, Arizona State University, Tempe, AZ, USA

    John C. H. Spence

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Diaspro, A. et al. (2019). Fluorescence Microscopy. In: Hawkes, P.W., Spence, J.C.H. (eds) Springer Handbook of Microscopy. Springer Handbooks. Springer, Cham. https://doi.org/10.1007/978-3-030-00069-1_21

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