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Using Immunochemical Methods to Analyze for Biomarkers of Exposure

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Part of the book series: NATO · Challenges of Modern Society ((NATS,volume 19))

Abstract

Studies assessing the exposure of workers, consumers, and others to pesticides are among the most costly aspects of assuring the safety of a pesticide. The analysis of samples is a significant portion of that cost (sample collection, sample preservation, transport, sample preparation, instrumental analysis time and data reduction). Information is presented here about an analytical technique that may be useful in providing data at a reduced cost. Immunochemical techniques used in the analysis of drugs and hormones in the clinical laboratory provide analytical information for a few dollars per analysis, whereas typical analytical costs for a pesticide in an environmental matrix may run from $50–$1000 per sample depending on the analyte, matrix and analytical method.

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References

  • Ames, B.N., Shigenaga, M.K., and Hagen, T.M., 1993, Oxidants, antioxidants, and the degenerative diseases of aging, Proc. Nad. Acad. Sci. USA 90: 7915.

    Article  CAS  Google Scholar 

  • Anis, N.A., and Eldefrawi, M.E., 1993, Reusable fiber optic immunosensor for rapid detection of imazethapyr herbicide, J. Agric. Food Chem. 41: 843.

    Article  CAS  Google Scholar 

  • Centeno, E.R., and Johnson, W.J., 1970, Antibodies to two common pesticides, DDT and malathion, Int. Arch. Allergy Appl. Immunol. 37: 1.

    Article  PubMed  CAS  Google Scholar 

  • Cheung, P.Y.K., and Hammock, B.D., 1988, Monitoring BT in the Environment with ELISA, in: “Biotechnology for Crop Protection,” P.A. Hedin, J.J. Menn and R.M. Hollingworth, eds., American Chemical Society, Washington, DC.

    Google Scholar 

  • de Frutos, M., and Regnier, F.E., 1993, Tandem chromatographic-immunological analyses, Anal. Chem. 65: 17.

    Google Scholar 

  • Dewey, M., Evans, D., Coleman, J., Priestley, R., Hull, R., Horsley, D., and Hawes, C., 1991, Antibodies in plant science, Acta Bot. Neerl. 40: 1.

    CAS  Google Scholar 

  • Eck, D.L., Kurth, M.J., and Macmillan, C., 1990, Trinitrotoluene and Other Nitroaromatic Compounds: Immunoassay Methods, in: “Immunochemical Methods for Environmental Analysis,” J.M. Van Emon and R.O. Mumma, eds., American Chemical Society, Washington, D.C.

    Google Scholar 

  • Engvall, E., and Perlmann, P., 1972, Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes, J. Immunol. 109: 129.

    PubMed  CAS  Google Scholar 

  • Feng, P.C.C., Horton, S.R., and Sharp, C.R., 1992, A general method for developing immunoassays to chloroacetanilide herbicides, J. Agric. Food Chem. 40: 211.

    Article  CAS  Google Scholar 

  • Gee, S.J., Hammock, B.D., and Van Emon, J.M., 1994, “A User’s Guide to Environmental Immunochemical Analysis”, Environmental Monitoring Systems Laboratory, U.S. Environmental Protection Agency, Las Vegas, NV 89109, EPA/540/R-94/509.

    Google Scholar 

  • Gee, Si.,. Miyamoto, T., Goodrow, M.H., Buster, D., and Hammock, B.D., 1988, Development of an enzyme-linked immunosorbent assay for the analysis of the thiocarbamate herbicide molinate, J. Agric. Food Chem. 36: 863.

    Article  Google Scholar 

  • Goodrow, M.H., Harrison, R.O., and Hammock, B.D., 1990, Hapten synthesis, antibody development, and competitive inhibition enzyme immunoassay for s-triazine herbicides, J. Agric. Food Chem. 38: 990.

    Article  CAS  Google Scholar 

  • Hall, J.C., Deschamps, R.J.A., and McDermott, M.R., 1990, Immunoassays to detect and quantitate herbicides in the environment, Weed Technol. 4: 226.

    CAS  Google Scholar 

  • Hammock, B.D., 1988, Applications of immunochemistry in crop protection and biotechnology, an overview, in: “Biotechnology for Crop Protection,” P.A. Hedin, J.J. Menn and R.M. Hollingworth, eds., American Chemical Society, Washington, DC.

    Google Scholar 

  • Hammock, B.D., and Mumma, R.O., 1980, Potential of immunochemical technology for pesticide analysis, in: “Recent Advances in Pesticide Analytical Methodology,”J. Harvey, Jr. and G. Zweig, eds., American Chemical Society, Washington, DC.

    Google Scholar 

  • Harrison, R.O., Gee, S.J., and Hammock, B.D., 1988, Immunochemical methods of pesticide analysis, in: “Biotechnology for Crop Protection,” P.A. Hedin, J.J. Menn and R.M. Hollingworth, eds., American Chemical Society, Washington, DC.

    Google Scholar 

  • Harrison, R.O., Goodrow, M.H., and Hammock, B.D., 1991, Competitive inhibition ELISA for the striazine herbicides: Assay optimization and antibody characterization, J. Agric. Food Chem, 39: 122.

    Article  CAS  Google Scholar 

  • Hayashi, Y., Shono, F., Yamamoto, S., Takasaki, W., Nakagawa, A., Watanabe, K., Yamashita, K., and Miyazaki, H., 1990, Immunoaffinity purification of 11-dehydro-thromboxane B2 from human urine and plasma for quantitative analysis by radioimmunoassay, Anal. Biochem. 187: 151.

    Article  PubMed  CAS  Google Scholar 

  • Jung, F., Gee, S.J., Harrison, R.O., Goodrow, M.H., Kam, A.E., Braun, A.L., Li, Q.X., and Hammock, B.D., 1989, Use of immunochemical techniques for the analysis of pesticides, Pest. Sci. 26: 303.

    Article  CAS  Google Scholar 

  • Kam, A.E., Harrison, R.O., Schmidt, D.J., Clarkson, C.E., Grassman, J., Goodrow, M.H., Lucas, A., Hammock, B.D., Van Emon, J.M., and White, R.J., 1991, Monoclonal immunoassay of triazine herbicides: development and implementation, in: “Immunoassays for Trace Chemical Analysis: Monitoring Toxic Chemicals in Humans, Food, and the Environment.,” M. Vanderlaan, L.H. Stanker, B.E. Watkins and D.W. Roberts, eds., American Chemical Society, Washington, DC.

    Google Scholar 

  • Katz, S.E., and Brady, M.S., 1990, High performance immunoaffinity chromatography for drug residue analysis, J. Assoc. Off. Anal. Chem. 73: 557.

    PubMed  CAS  Google Scholar 

  • Krämer, P.M., Li, Q.X., and Hammock, B.D., 1993, Integration of LC with immunoassay: an approach combining the potential of both methods, J. A.O.A.C. Int., 77: 1275.

    Google Scholar 

  • Landsteiner, K., 1945, “The Specificity of Serological Reactions,” Harvard University Press, Cambridge, Massachusetts.

    Google Scholar 

  • Langone, J.J., and van Vunakis, H., 1975, Radioimmunoassay for dieldrin and aldrin, Res. Commun. Chem. Pathol. Pharmacol. 10: 163.

    PubMed  CAS  Google Scholar 

  • Lawruk, T.S., Lachman, C.E., Jourdan, S.W., Fleeker, J.R., Herzog, D.P., and Rubio, F.M., 1993, Determination of metolachlor in water and soil by a rapid magnetic particle-based ELISA, J. Agric. Food Chem. 41: 1426.

    Article  CAS  Google Scholar 

  • Lee, L.S., Wall, J.H., Cotty, P.J., and Bayman, P., 1990, Integration of enzyme-linked immunosorbent assay with conventional chromatographic procedures for quantitation of aflatoxin in individual cotton bolls, seeds, and seed sections, J. Assoc. Off. Anal. Chem. 73: 581.

    PubMed  CAS  Google Scholar 

  • Li, Q., Zhao, M., Gee, S.J., Kurth, M., Seiber, J.N., and Hammock, B.D., 1991, Development of enzyme-linked immunosorbent assays for 4-nitrophenol and substituted 4-nitrophenols, J. Agric. Food Chem. 39: 1685.

    Article  CAS  Google Scholar 

  • Lucas, A.D., Jones, A.D., Goodrow, M.H., Saiz, S.G., Blewett, C., Seiber, J.N., and Hammock, B.D., 1993, Determination of atrazine metabolites in human urine: Development of a biomarker of exposure, Chem. Res. ToxicoL 6: 107.

    Article  PubMed  CAS  Google Scholar 

  • Lucas, A.D., Schneider, P., Harrison, R.O., Seiber, J.N.,Hammock, B.D., Biggar, J.W., and Rolston, D.E., 1992, Determination of atrazine and simazine in water and oil using polyclonal and monoclonal antibodies in enzyme-linked immunosorbent assays, Food Agric. Immunol. 3:155.

    Google Scholar 

  • Marco, M.-P., Gee, S.J., Cheng, H.M., Liang, Z.Y., and Hammock, B.D., 1993, Development of an enzyme-linked immunosorbent assay for carbaryl, J. Agric. Food Chem. 41: 423.

    Article  CAS  Google Scholar 

  • McDiarmid, M.A., and Strickland, P.T., 1990, DNA adducts as markers of exposure in hazardous waste workers, Occ. Med. 5: 49.

    CAS  Google Scholar 

  • Rodbard, D., 1981, Mathematics and statistics of ligand assays: an illustrated guide, in: “Ligand Assay: Analysis of International Developments on Isotopic and Nonisotopic Immunoassay,” J. Langan and J.J. Clapp, eds., Masson Publishing USA, Inc., New York.

    Google Scholar 

  • Schlaeppi, J., Meyer, W., and Ramsteiner, K.A., 1992, Determination of triasulfuron in soil by monoclonal antibody-based enzyme immunoassay, J. Agric. Food Chem. 40: 1093.

    Article  CAS  Google Scholar 

  • Schneider, P., and Hammock, B.D., 1992, Influence of the ELISA format and the hapten-enzyme conjugate on the sensitivity of an immunoassay for s-triazine herbicides using monoclonal antibodies, J. Agric. Food Chem. 40: 525.

    Article  CAS  Google Scholar 

  • Skerritt, J.H., Hill, A.S., Beasley, H.L., Edward, S.L., and McAdam, D.P., 1992, Enzyme-linked immunosorbent assay for quantitation of organophosphate pesticides: fenitrothion, chlorpyrifosmethyl, and pirimiphos-methyl in wheat grain and flour-milling fractions, J. A.O.A.C. Int. 75: 519.

    CAS  Google Scholar 

  • Stanker, L.H., Bigbee, C., Van Emon, J., Watkins, B., Jense, R.H., Morris, C., and Vanderlaan, M., 1989, An immunoassay for pyrethroids: Detection of permethrin in meat, J. Agric. Food Chem. 37: 834.

    Article  CAS  Google Scholar 

  • Stanker, L.H., Watkins, B., Rogers, N., and Vanderlaan, M., 1987, Monoclonal antibodies for dioxin: antibody characterization and assay development, ToxicoL 45: 229.

    Article  CAS  Google Scholar 

  • Tijssen, P., 1985, “Practice and Theory of Enzyme Immunoassays,” Elsevier, Amsterdam. Trucksess, M.W., Young, K., Donahue, K.F., Morris, D.K. and Lewis, E., 1990, Comparison of twoimmunochemical methods with thin-layer chromatographic methods for determination of aflatoxins, J. Assoc. Off. Anal. Chem. 73: 425.

    Google Scholar 

  • Van Emon, J., Hammock, B., and Seiber, J.N., 1986, Enzyme-linked immunosorbant assay for paraquat and its application to exposure analysis, Anal. Chem. 58: 1866.

    Article  PubMed  Google Scholar 

  • Van Emon, J.M., and Lopez-Avila, V., 1992, Immunochemical methods for environmental analysis, Anal. Chem. 64: 79.

    Article  Google Scholar 

  • Voller, A., Bidwell, D.E., and Bartlett, A., 1976, Enzyme immunoassay in diagnostic medicine. Theory and practice, Bull. World Health Organ. 53: 55.

    PubMed  CAS  Google Scholar 

  • Wie, S.I., and Hammock, B.D., 1984, Comparison of coating and immunizing antigen structure on the sensitivity and specificity of immunoassays for benzoylphenylurea insecticides, J. Agric. Food Chem. 32: 1294.

    Article  CAS  Google Scholar 

  • Wing, K.D., Hammock, B.D., and Wustner, D.A., 1978, Development of an S-bioallethrin specific antibody, J. Agric. Food Chem. 26: 1328.

    Article  PubMed  CAS  Google Scholar 

  • Winter, G., and Milstein, C., 1991, Man-made antibodies, Nature 349: 293.

    Article  PubMed  CAS  Google Scholar 

  • Wittmann, C., and Hock, B., 1993, Analysis of atrazine residues in food by an enzyme immunoassay, J. Agric. Food Chem. 41: 1421.

    Article  CAS  Google Scholar 

  • Wong, R.B., and Ahmed, Z.H., 1992, Development of an enzyme-linked immunosorbent assay for imazaquin herbicide, J. Agric. Food Chem. 40: 811.

    Article  CAS  Google Scholar 

  • Wylie, D.E., Carlson, L.D., Carlson, R., Wagner, F.W., and Schuster, S.M., 1991, Detection of mercuric ions in water by ELISA with a mercury-specific antibody, Anal. Biochem. 194: 381.

    Article  PubMed  CAS  Google Scholar 

  • Yalow, R.S., and Berson, S.A., 1959, Radiobiology: Assay of plasma insulin human subjects by immunological methods, Nature 184: 1648.

    Article  PubMed  CAS  Google Scholar 

  • Yalow, R.S., and Berson, S.A., 1960, Immunoassay of endogenous plasma insulin in man, J. Clin. Invest. 39: 1157.

    Article  PubMed  CAS  Google Scholar 

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Gee, S.J., Lucas, A.D., Hammock, B.D. (1995). Using Immunochemical Methods to Analyze for Biomarkers of Exposure. In: Curry, P.B., Iyengar, S., Maloney, P.A., Maroni, M. (eds) Methods of Pesticide Exposure Assessment. NATO · Challenges of Modern Society, vol 19. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0973-2_16

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  • DOI: https://doi.org/10.1007/978-1-4899-0973-2_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0975-6

  • Online ISBN: 978-1-4899-0973-2

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