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Tissue Renin-Angiotensin Systems pp 311–317Cite as

Cloning, Expression and Regulation of Angiotensin II Receptors

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 377))

Summary

Complementary DNAs for angiotensin II type 1 receptor isoforms AT1A and AT1B were cloned by expression cloning from bovine adrenal and rat vascular smooth muscles. Human AT, receptor was also cloned. Seven transmembrane structures emerged. The AT1 type receptor interacted with more than one type of G-proteins. The ligand binding site of AT1 involving Arg167, Lys199, and Asp263 has been identified by site directed mutagenesis. The regulation of the receptors occur at many stages. The isoform, AT2, was also expression cloned from rat pheochromocytoma cells. Although its ligand binding is not affected by stable GTP analogs, it is a seven transmembrane domain receptor. It mediates the modulations of phosphotyrosine phosphatase by angiotensin II and AT2 specific CGP42112A. The modulation was abolished by pertussis toxin. Thus, AT2 belongs to a new class of angiotensin receptors with unique signalling and regulatory mechanisms. AT1 mediates cellular growth. Interestingly, AT2 expression is inversely related to the mitogenic activity of cells.

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Authors and Affiliations

  1. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232-0146, USA

    Tadashi Inagami, Yoshiaki Yamano, Smriti Bardhan, Shigeyuki Chaki, Deng-Fu Guo, Kenji Ohyama, Yoshikazu Kambayashi, Kyoko Takahashi, Toshihiro Ichiki, Satoshi Tsuzuki & Hua Tang

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  1. Tadashi Inagami
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  2. Yoshiaki Yamano
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  3. Smriti Bardhan
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  4. Shigeyuki Chaki
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  5. Deng-Fu Guo
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  6. Kenji Ohyama
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  7. Yoshikazu Kambayashi
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  8. Kyoko Takahashi
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  9. Toshihiro Ichiki
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  10. Satoshi Tsuzuki
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  11. Hua Tang
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Editor information

Editors and Affiliations

  1. Institute for Hormone and Fertility Research, University of Hamburg, Hamburg, Germany

    Amal K. Mukhopadhyay Ph.D.

  2. University of Florida, Gainesville, Florida, USA

    Mohan K. Raizada Ph.D.

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© 1995 Springer Science+Business Media New York

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Inagami, T. et al. (1995). Cloning, Expression and Regulation of Angiotensin II Receptors. In: Mukhopadhyay, A.K., Raizada, M.K. (eds) Tissue Renin-Angiotensin Systems. Advances in Experimental Medicine and Biology, vol 377. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0952-7_21

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  • DOI: https://doi.org/10.1007/978-1-4899-0952-7_21

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0954-1

  • Online ISBN: 978-1-4899-0952-7

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