Abstract
The effect of phosphatidylserine (PtdSer) administration (15 mg/kg i. p.) for 30 days was investigated on acetylcholine (ACh) release from electrically stimulated cortical slices and on 45Ca2+ uptake into K+-depolarized cortical synaptosomes in 3 and 22–24 month old rats. ACh release in 24 month old rats treated with the solvent was 50% lower than in 3 month old rats at all stimulation frequencies tested. On the contrary, no significant decrease in ACh release was found in the 24 month old rats treated with PtdSer. Similarly, the decrease in 45Ca2+ uptake found in the 22 month old rats treated with the solvent did not occur in the aging rats chronically treated with PtdSer. The possibility is envisaged that PtdSer may act by influencing age-dependent changes in membrane phospholipid composition.
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References
Bartus RT, Dean RL, Beer B, Lippa AS (1982) The cholinergic hypothesis of geriatric memory dysfunction. Science 217: 408–409.
Bruni A, Toffano G (1985) Influence of serine phospholipids on biogenic amine secretion in vivo and in vitro. In: Horrocks LA (ed): Phospholipids in the nervous system. Vol 2: Physiological roles. Raven Press, New York, pp. 21–29.
Casamenti F, Mantovani P, Amaducci L, Pepeu G (1979) Effect of phosphatidylserine on acetylcholine output from the cerebral cortex of the rat. J Neurochem 32: 529–533.
Drago F, Canonico PL, Scapagnini U (1931) Behavioral effects of phospatidylserine in aged rats. Neurobiol Aging 2: 209–213.
Feinstein MB (1964) Reaction of local anesthetics with phospholipids. A possible chemical basis for anesthesia. J Gen Physiol 48: 357–374.
Gibson GE, Peterson C (1981) Aging decreases oxidative metabolism and the release and synthesis of acetylcholine. J Neurochem 39: 978–984.
Goodman FR, Weiss GB, Goth A (1976) Alteration by phosphatidylserine of tension responses and 45Ca distribution in aortic smooth muscle. J Pharmacol Exp Ther 198: 168–175.
Goth A, Adams HR, Knoohuizen M (1971) Effect of calcium on dextran-induced histamine release from isolated mast cells. Br J Pharmac 46: 767–769.
Hershkowitz M (1983) Mechanisms of brain-the role of membrane fluidity. In: Gispen WH, Traber J (eds): Aging of the brain. Elsevier, Amsterdam; pp. 101–114.
Leon A, Benvegnù D, Toffano G, Orlando P, Massari P (1978) Effect of brain cortex phospholipids on adenylate-cyclase acitivity of mouse brain. J Neurochem 30: 23–26.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–285.
Mantovani P, Aporti F, Bonetti AC, Pepeu G (1982) Effects of phosphatidylserine on brain cholinergic mechanisms. In: Horrocks LA, Ansell GB, Porcellati G (eds): Phospholipids in the nervous system. Vol. 1: Metabolism. Raven Press, New York; pp. 165–172.
Mongar JL, Svec P (1972) The effect of phospholipids on anaphylactic histamine release Br J Pharmac 46: 741–752.
Pedata F, Slavikova J, Kotas A, Pepeu G (1983) Acetylcholine release from rat cortical slices during postnatal development and aging. Neurobiol Aging 4: 31–35.
Pepeu G, Gori G, Bartolini L (1980) Pharmacologic and therapeutic perspectives on dementia: an experimental approach. In: Amaducci L, Davison AN, Antuono P (eds): Aging of the brain and dementia. Raven Press, New York; pp. 271–274.
Peterson C, Gibson GE (1983) Aging and 3,4-diaminopyridine alter synaptosomal calcium uptake. J Biol Chem 258: 11482–11486.
Toffano G, Leon A, Benvegnu D, Boarato E, Azzone GF (1976) Effect of brain cortex phospholipids on catecholamine content of mouse brain. Pharmacol Res Comm 8: 581–590.
Toffano G, Bruni A (1980) Pharmacological properties of phospholipid liposomes. Pharmacol Res Comm 12: 829–845.
Toffano G, Battistella A, Mazzari S, Orlando P, Massari P, Giordano C (1982) Fate of phosphatidyl-L-(U-14C)-serine in mice. In: Horrocks LA, Ansell GB, Porcellati G (eds): Metabolism. Raven Press, New York; pp. 173–180.
White JR, Ishizaka T, Ishizaka K, Sha’afi RI (1984) Direct demonstration of increased intracellular concentration of free calcium as measured by quin-2 in stimulated rat peritoneal mast cell. Proc Natl Acad Sci 81: 3978–3982.
Wu PH, Phillis JW, Thierry DL (1982) Adenosine receptor agonists inhibit K+-evoked Ca2+ uptake by rat brain cortical synaptosomes. J Neurochem 39: 700–708.
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© 1986 Springer-Verlag Berlin Heidelberg
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Pepeu, G., Giovannelli, L., Giovannini, M.G., Pedata, F. (1986). Effect of Phosphatidylserine on Cortical Acetylcholine Release and Calcium Uptake in Adult and Aging Rats. In: Horrocks, L.A., Freysz, L., Toffano, G. (eds) Phospholipid Research and the Nervous System. FIDIA Research Series, vol 4. Springer, New York, NY. https://doi.org/10.1007/978-1-4899-0490-4_26
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DOI: https://doi.org/10.1007/978-1-4899-0490-4_26
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