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In Planta Metabolic State of Neotyphodium Endophyte Mycelium Assessed Through Use of the GUS Reporter Gene in Combination with Hyphal Enumeration

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Neotyphodium/Grass Interactions

Abstract

We have recently developed methods for the quantitative measurement of B-glucuronidase (GUS) reporter gene activity of transformants of Neotyphodium endophytes during their growth in perennial ryegrass (2). When transformants express the reporter gene constitutively, GUS activity can be used as an indicator of the distribution of in planta endophyte metabolic activity (2). Because the concentration of endophyte metabolic activity in grass tissue is a result of both the concentration of endophyte hyphae and their metabolic state, we have now combined GUS assays with methods for the determination of biovolume of endophyte hyphae in plant sections to assess the metabolic state of the endophyte mycelium.

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References

  • Christensen, M. J., A. Leuchtmann, D. D. Rowan, and B. A. Tapper. 1993. Taxonomy of Acremonium endophytes of tall fescue (Festuca arundinacea), meadow fescue (F. pratensis), and perennial rye-grass (Lolium perenne). Mycolog. Res. 97: 1083–1092.

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  • Herd, S., M. J. Christensen, K. Saunders, D. B. Scott, and J. Schmid. 1996. Quantitative assessment of in planta distribution of metabolic activity and gene expression of endophytic fungi. Microbiology in press.

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Authors and Affiliations

  1. Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand

    Y. Y. Tan, M. Spiering, K. Saunders & J. Schmid

  2. Department of Microbiology and Genetics, AgResearch Grasslands, Palmerston North, New Zealand

    M. J. Christensen

Authors
  1. Y. Y. Tan
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  2. M. Spiering
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  3. M. J. Christensen
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  4. K. Saunders
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  5. J. Schmid
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Editor information

Editors and Affiliations

  1. Agricultural Research Service, United States Department of Agriculture, Athens, Georgia, USA

    Charles W. Bacon

  2. Department of Crop and Soil Sciences, The University of Georgia, Athens, Georgia, USA

    Nicholas S. Hill

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© 1997 Springer Science+Business Media New York

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Tan, Y.Y., Spiering, M., Christensen, M.J., Saunders, K., Schmid, J. (1997). In Planta Metabolic State of Neotyphodium Endophyte Mycelium Assessed Through Use of the GUS Reporter Gene in Combination with Hyphal Enumeration. In: Bacon, C.W., Hill, N.S. (eds) Neotyphodium/Grass Interactions. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0271-9_15

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  • DOI: https://doi.org/10.1007/978-1-4899-0271-9_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0273-3

  • Online ISBN: 978-1-4899-0271-9

  • eBook Packages: Springer Book Archive

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