Abstract
The initial step in the remodeling pathway of PAF synthesis is catalyzed by phospholipase A2 (PLA2).1 It has generally been accepted that PLA2 acts directly on 1-0-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine (alkyl-2-AA-GPC) to form lyso-PAF (1-alkyl-2-lyso-GPC) which is then acetylated to yield PAF. More recent studies suggest that PAF synthesis may also be initiated indirectly through the action of CoA-independent transacylase following the hydrolysis of l-0-alk-1′-enyl-2-AA-sn-glycero-3-phosphoethanolamine (alkenyl-2-AA-GPE) by PLA2.2,3 In the putative indirect pathway, an accumulation of alkenyl-2-lyso-GPE triggers the transfer of AA from alkyl-2-AA-GPC to the alkenyl-2-lyso-GPE thus forming lyso-PAF. It seems logical that both the direct and indirect pathways may contribute to PAF synthesis. However, the relative physiological importance of the two pathways has not been established.
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Wykle, R.L., Wijkander, J., Nixon, A.B., Daniel, L.W., O’Flaherty, J.T. (1996). Activation of 85 kDa PLA2 by Eicosanoids in Human Neutrophils and Eosinophils. In: Nigam, S., Kunkel, G., Prescott, S.M. (eds) Platelet-Activating Factor and Related Lipid Mediators 2. Advances in Experimental Medicine and Biology, vol 416. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0179-8_52
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DOI: https://doi.org/10.1007/978-1-4899-0179-8_52
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