Skip to main content

Microbiology of Denitrification and Other Processes Involving the Reduction of Oxygenated Nitrogenous Compounds

  • Chapter
Denitrification in the Nitrogen Cycle

Part of the book series: NATO Conference Series ((E,volume 9))

Abstract

Considerable changes take place in the energy metabolism of the soil microflora when oxygen becomes a limiting factor. Aerobic respiration is replaced by two other phenomena, namely fermentation and anaerobic respiration. In fermentation the transfer of electrons is associated with energy metabolism and occurs by means of internal redox reactions involving organic molecules and substrate-level phosphorylations. The Krebs cycle does not operate and growth is slow. In contrast, during anaerobic respiration oxygen is replaced by an electron acceptor of inorganic origin. In this type of respiration, which only occurs in procaryotes, the Krebs cycle functions in association with an electron transport chain which permits oxidative phosphorylation and more extensive growth than fermentation.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Balderston, W.L., Sherr, B., and Payne, W.J., 1976, Blockage by acetylene of nitrous oxide reduction in Pseudomonas perfectomarinus, Appl. Environ. Microbiol., 31: 504.

    Google Scholar 

  • Blackmer, A.M., Bremner, J.M., and Schmidt, E.L., 1980, Production of nitrous oxide by ammonia oxidizing chemoautotrophic microorganisms in soil, Appl. Environ. Microbiol., 40: 1060.

    Google Scholar 

  • Bollag, J.M., and Tung, G., 1972, Nitrous oxide release by soil fungi, Soil Biol. Biochem., 4: 271.

    Google Scholar 

  • Breitenbeck, G.A., Blackmer, A.M., and Bremner, J.M., 1980, Effects of different nitrogen fertilizers on emission of nitrous oxide from soil, Geophys. Res. Letters, 7: 85.

    Google Scholar 

  • Bremner, J.M., and Shaw, K., 1958, Denitrification in soil, J. Agric. Sci., 51: 22.

    Google Scholar 

  • Bremner, J.M., and Blackmer, A.M., 1979, Effects of acetylene and soil water content on emission of nitrous oxide from soils, Nature, 280: 380.

    Article  ADS  Google Scholar 

  • Bremner, J.M., and Blackmer, A.M., 1981, Terrestrial nitrification as a source of atmospheric nitrous oxide, in: “Denitrification, nitrification and atmospheric nitrous oxide”, C.C. Delwiche, ed., John Wiley and Sons, New York.

    Google Scholar 

  • Bremner, J.M., Breitenbeck, G.A., and Blackmer, A.M., 1981a, Effect of nitrapyrin on emission of nitrous oxide from soil fertilizer with anhydrous ammonia, Geophys. Res. Letters, 8: 353.

    Article  ADS  Google Scholar 

  • Bremner, J.M., Breitenbeck, G.A., and Blackmer, A.M., 1981b, Effects of anhydrous ammonia fertilization on emission of nitrous oxide from soils, J. Environ. Qual., 10: 77.

    Google Scholar 

  • Bryan, B.A., 1981, Physiology and biochemistry of denitrification, in: “Denitrification, nitrification and atmospheric nitrous oxide”, C.C. Delwiche, ed., John Wiley and Sons, New York.

    Google Scholar 

  • Buresh, R.J., and Patrick, W.H., 1981, Nitrate reduction to ammonium and organic nitrogen in the estuarine sediment, Soil Biol. Biochem., 13: 279.

    Google Scholar 

  • Caskey, W.H., and Tiedje, J.M., 1979, Evidence for Clostridia as agents of dissimilatory reduction of nitrate to ammonium in soils, Soil Sci. Soc. Am. J., 43: 391.

    Google Scholar 

  • Caskey, W.H., and Tiedje, J.M., 1980, The reduction of nitrate to ammonium by a Clostridium sp. isolated from soil, J. Gen. Microbiol., 119: 217.

    Google Scholar 

  • Corbet, A.S., 1935, The formation of hyponitrous acid as an intermediate compound in the biological or photochemical oxidation of ammonia to nitrous acid. II. Microbiological oxidation, Biochem. J., 29: 1086.

    Google Scholar 

  • Crutzen, P.J., and Ehhalt, D.H., 1976, Effects of nitrogen fertilizers and combustion on the stratospheric ozone layer, Ambio, 6: 112.

    Google Scholar 

  • Daniel, R.M., Steele, K.W., and Limmer, A.W., 1980b, Denitrification by Rhizobia. A possible factor contributing to nitrogen losses from soils, New Zealand Agricult. Sci., 14: 109.

    Google Scholar 

  • Daniel, R.M., Smith, I.M., Phillip, J.A.D., Ruteliffe, M.D., Drozd, J.W., and Bull, A.T., 1980a, Anaerobic growth and denitrification by Rhizobium japonicum and other Rhizobia, J. Gen. Microbiol., 120: 517.

    Article  Google Scholar 

  • Daniel, R.M., Zimmer, A.W., Steele, K.W., and Smith, I.M., 1982, Aerobic growth, nitrate reduction and denitrification in 46 Rhizobium strains, J. Gen. Microbiol., 128: 1811.

    Google Scholar 

  • Firestone, M.K., Firestone, R.B., and Tiedje, J.M., 1979, Nitric oxide as an intermediate in denitrification: evidence from nitrogen - 13 isotope exchange, Biochem. Biophys. Res. Comm., 91: 10.

    Google Scholar 

  • Freney, J.R., Denmead, 0.T., and Simpson, J.R., 1979, Nitrous oxide emission from soils at low moisture contents, Soil Biol. Biochem., 11: 167.

    Google Scholar 

  • Gamble, R.N., Betlach, M.R., and Tiedje, J.M., 1977, Numerically dominant denitrifying bacteria from world soils, Appl. Environ. Microbiol., 33: 926.

    Google Scholar 

  • Garber, E.A.E., and Hollocher, T.C., 1981, 15N tracer studies on the role of NO in denitrification, J. Biol. Chem., 256: 5459.

    Google Scholar 

  • Garcia, J.L., 1977, Analyse des différents groupes composant la microflore dénitrifiante des sols de rizière du Sénégal, Ann. Microbiol. (Inst. Pasteur), 128A: 433.

    Google Scholar 

  • Garcia, J.L., 1978, Etude systématique de souches pures isolées, in: “Etude de la dénitrification dans les sols de rizières du Sénégal”, Thèse Doct. Sci., Marseille.

    Google Scholar 

  • Garcia, J.L., Roussos, S., and Bensoussan, M., 1981, Etude taxonomique de bactéries dénitrifiantes isolées sur benzoate dans les sols de rizières du Sénégal, Cah. ORSTOM, Sér. Biol., 43: 13.

    Google Scholar 

  • Gilbert, R.G., Lance, J.C., and Miller, J.B., 1979, Denitrifying bacteria populations and nitrogen removal is soil columns intermittently flooded with secondary sewage effluent, J. Environ. Qual., 8: 101.

    Google Scholar 

  • Hall, J.B., 1978, Nitrate-reducing bacteria, in: “Microbiology”, Schlessinger, ed., American Society for Microbiology, Washington (cited by Ingraham).

    Google Scholar 

  • Hasan, S.M., and Hall, J.B., 1975, The physiological function of nitrate reduction in Clostridium perfringens, J. Gen. Microbiol., 87: 120.

    Google Scholar 

  • Ingraham, J.L., 1981, Microbiology and genetics of denitrifiers, in: “Denitrification, nitrification, and atmospheric nitrous oxide”, C.C. Delwiche, ed., John Wiley and Sons, New York.

    Google Scholar 

  • Ishizawa, S., 1980, Note on nitrate reduction in Rhizobium, Soil Sci. Plant Nutr., 26: 447.

    Google Scholar 

  • Ishizawa, S., 1939, On the consumption of inorganic nitrogen by root nodule bacteria of leguminous plants, J. Soil Sci. Manure, Japan, 13:135 and 13: 560 (Cited by Ishizawa, 1980).

    Google Scholar 

  • Jacobson, S.N., and Alexander, M., 1980, Nitrate loss from soil in relation to temperature, carbon source, and denitrifiers populations, Soil Biol. Biochem., 12: 501.

    Google Scholar 

  • Kemp, J.D., Atkinson, D.E., Ehret, A., and Lazzarini, R.A., 1963, Evidence for identity of the NADP specific sulfite and nitrite reductases of E. coli, J. Biol. Chem., 238: 3466 (cited by Yordi and Ruoff).

    Google Scholar 

  • Koike, I., and Hattori, A., 1975, Energy yield of denitrification: an estimate from growth yield in continuous cultures of Pseudomonas denitrificans under nitrate, nitrite and nitrous oxidelimited conditions, J. Gen. Microbiol., 88: 11.

    Article  Google Scholar 

  • Koike, I., and Hattori, A., 1978, Denitrification and ammonia formation in anaerobic coastal sediments, Appl. Environ. Microbiol., 35: 278.

    Google Scholar 

  • Murphy, S.G., and Elkan, G.H., 1965, Nitrogen metabolism of some strains of Rhizobium japonicum having different nodulating capacities, Can. J. Microbiol., 11: 1039.

    Google Scholar 

  • Ndmmick, H., 1956, Investigation on denitrification in soil, Acta Agricult. Scand., 13: 195.

    Google Scholar 

  • Payne, W.J., 1973, Reduction of nitrogenous oxide by microorganisms, Bact. Rev., 37: 409

    Google Scholar 

  • Payne, W.J., Rowe, J.J., and Sherr, B.F., 1980, Denitrification: a plea for attention, in: “Nitrogen fixation”. I., W.E. Newton and W.H. Orme-Johnson, ed., Univ. Park Press, Baltimore.

    Google Scholar 

  • Pichinoty, F., 1973, La réduction bactérienne des composés oxygénés mineraux de l’azote, Bull. Inst. Pasteur, 71: 317.

    Google Scholar 

  • Ponnamperuma, F.N., 1972, The chemistry of submerged soils, Adv. Agron., 24: 29.

    Article  Google Scholar 

  • Rigaud, J., Bergersen, F.J., Turner, G.L., and Daniel, R.M., 1973, nitrate dependent anaerobic acetylene-reduction and nitrogen fixation by soyabean bacteroids, J. Gen. Microbiol., 77: 137.

    Google Scholar 

  • Richtie, G.A.F., and Nicholas, D.J.D., 1972, Identification of the sources of nitrous oxide produced by oxidative and reductive processes in Nitrosomonas Europaea, Biochem. J., 126: 1181 (cited by Bremner and Blackmer).

    Google Scholar 

  • Richtie, G.A.F., and Nicholas, D.J.D., 1974, The partical characterization of purified nitrite reductase and hydroxylamine oxidase from Nitrosomonas Europaea, Biochem. J., 138: 471 (cited by Bremner and Blackmer, 1981).

    Google Scholar 

  • Scott, D.B., Scott, D.A., and Döbereiner, 1979, Nitrogenase activity and nitrate respiration in Azospirillum sp., Arch. Microbiol. 121: 141.

    Google Scholar 

  • Sias, S.R., Stouthamer, A.H., and Ingraham, J.L., 1980, The assimilatory and dissimilatory nitrate reductases of Pseudomonas aeruginosa are encoded by different genes, J. Gen. Bact., 118: 229.

    Google Scholar 

  • Sikora, L.S., and Keeney, D.R., 1976, Evolution of sulfur - Thiobacillus denitrificans nitrate removal system, J. Environ. Qual. 5: 298.

    Google Scholar 

  • Smith, M.S., and Zimmerman, K., 1981, Nitrous oxide production by non denitrifying soil nitrate reducers, Soil Sci. Soc. Am. J., 45: 865.

    Google Scholar 

  • Sorensen, J., 1978, Capacity for denitrification and reduction of nitrate to ammonia in a coastal marine sediment, Appl. Environ. Microbiol., 35: 301.

    Google Scholar 

  • Stanford, G., Legg, J.0., Dzienia, S., and Simpson, E.C., 1975, Denitrification and associated nitrogen transformations in soils, Soil Sci, 120: 147.

    Google Scholar 

  • Stanier, R.Y., Palleroni, N.J., and Doudoroff, M., 1966, The aerobic Pseudomonas: a taxonomic study, J. Gen. Microbiol., 43: 159 (cited by Ingraham).

    Google Scholar 

  • Taylor, B.F., and Heeb, M.J., 1972, The anaerobic degradation of aromatic compounds by a denitrifying bacterium. Radioisotope and mutant studies, Arch. Microbiol., 83: 165.

    Google Scholar 

  • Tiedje, J.M., Caskey, N.V., Smith, M.S., Bleakley, B.H., and Firestone, R.B., 1979, Nitrous oxide production by bacteria that reduce nitrate to nitrite,1 gron. +~str., 165. Tiedje, J.M., 1981, Use of N and N in studies on the dissimilatory fate of nitrate, in: “Genetic engineering of symbiotic nitrogen fixation and conservation to fixed nitrogen”, Plenum Press, New York.

    Google Scholar 

  • Van Hartingsveldt, J., and Stouthamer, A.H., 1973, Mapping and characterization of mutants of Pseudomonas aeruginosa affected in nitrite respiration in aerobic or anaerobic growth, J. Gen. Microbiol., 74: 97.

    Article  Google Scholar 

  • Verhoeven, W., 1956, Studies on true dissimilatory nitrate reduction, Ant. van Leeuwen, J. Microbiol. Serol., 22: 385.

    Article  Google Scholar 

  • Volz, M.G., Belser, L.W., Ardakani, M.S., and Mc Laren, A.D., 1975, Nitrate reduction and associated microbial populations in a ponded handford sandy loam, J. Environ. Qual., 4: 99.

    Google Scholar 

  • Volz, M.G., 1977, Assessing two diagnostic methods for enumeration of nitrate reducing and denitrifying bacteria in soil-plant root association, Soil Sci. Soc. Am. J., 41: 337.

    Google Scholar 

  • Volz, M.G., and Starr, J.L., 1977, Nitrate dissimilation and population dynamics of denitrifying bacteria during short term continuous flow, Soil Sci. Soc. Am. J., 41: 891.

    Google Scholar 

  • Wijler, J., and Delwiche, C.C., 1954, Investigations on the denitrifying process in soil, Plant and Soil, 5: 155.

    Article  Google Scholar 

  • Yordy, D.M., and Ruoff, K.L., 1981, Dissimilatory nitrate reduction to ammonia, in: “Denitrification, nitrification and atmospheric nitrous oxide”, C.C. Delwiche, ed., John Wiley and Sons, New York.

    Google Scholar 

  • Yoshida, T., and Alexander, M., 1970, Nitrous oxide formation by Nitrosomonas Europaea and heterotrophic microorganisms, Soil Sci. Soc. Am. J., 34: 880.

    Google Scholar 

  • Yoshinari, T., and Knowles, R., 1976, Acetylen inhibition of nitrous oxide reduction by denitrifying bacteria, Biochem. Biophys. Res. Comm., 69: 705.

    Google Scholar 

  • Zablotowicz, R.M., Eskew, D.L., and Focht, D.D., 1978, Denitrification in Rhizobium, Can. J. Microbiol., 24: 757.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer Science+Business Media New York

About this chapter

Cite this chapter

Germon, J.C. (1985). Microbiology of Denitrification and Other Processes Involving the Reduction of Oxygenated Nitrogenous Compounds. In: Golterman, H.L. (eds) Denitrification in the Nitrogen Cycle. NATO Conference Series, vol 9. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9972-9_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9972-9_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9974-3

  • Online ISBN: 978-1-4757-9972-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics