Abstract
Glutamine synthetase (GS) from E. coli requires two divalent metal ions per active site in order to catalyze the ATP-dependent formation of glutamine from glutamate and ammonia and plays a central role in ammonia metabolism in both prokaryotic and eukaryotic systems.
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Abbreviations
- GS:
-
glutamine synthetase
- GS n=2:
-
low adenylylation state enzyme
- GS n=12:
-
high adenylylation state enzyme
- Hepes:
-
N-(2-hydroxyethyl)piperazine-N′ -2-ethanesulfonic acid
- PEP:
-
phosphoenol pyruvate
- EDTA:
-
ethylene diamine tetraacetic acid
- Pipes:
-
piperazine-N, N′ -bis(2-ethanesulfonic acid)
References
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Abell, L.M., Villafranca, J.J. (1990). Effect of Metal Ions and Adenylylation State on the Energetics of the E. Coli Glutamine Synthetase Reaction. In: Baldwin, T.O., Raushel, F.M., Scott, A.I. (eds) Chemical Aspects of Enzyme Biotechnology. Industry-University Cooperative Chemistry Program Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9637-7_1
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