Skip to main content
SpringerLink
Log in

Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired-Tracer Dilution: A Review of Recent Studies

  • Chapter
Book cover Cell Membrane Transport

  • 103 Accesses

Abstract

Carrier-mediated transport of blood-borne substrates at the blood-tissue interface in the microcirculation is a very rapid process and influx of labelled molecules can often be measured within the time of a single circulation through an organ. The plasma membranes involved in the tracer uptake may be those of (i) the endothelial cell, (ii) parenchymal cell and (iii) neuronal terminals.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Barros, L.F., Bustamante, J.C., Yudilevich, D.L. and Jarvis, S.M. 1990. Adenosine transport and nitrobenzylthioinosine binding in human placental membrane vesicles from brush-border and basal sides of the trophoblast. J. Membr. Biol. 117, in press.

    Google Scholar 

  • Betz, A.L., Gilboe, D.D., Yudilevich, D.L. and Drewes, L.R. 1973. Kinetics of unidirectional glucose transport into the isolated dog brain. Am. J. Physiol. 225: 586–592.

    PubMed  CAS  Google Scholar 

  • Betz, A.L., Gilboe, D.D. and Drewes, L.R. 1975. Kinetics of unidirectional leucine transport into brain: effects of isoleucine, valine, and anoxia. Am. J. Physiol. 228: 895–900.

    Google Scholar 

  • Boyd, C.A.R. and Yudilevich, D.L. 1987. Blood-tissue movement of amino acids in various organs: the basolateral membrane and its importance in the polarity of transepithelial transport. In: Amino Acid Transport in Animal Cells. Eds. D.L. Yudilevich and C.A.R. Boyd, pp. 120–156, Manchester University Press, Manchester

    Google Scholar 

  • Bravo, I. and Sobrevía, L.. Kinetics of the L-alanine transport through the basolateral membrane in isolated oxyntic glands. Biochim. Biophys. Acta (in press).

    Google Scholar 

  • Bravo, I., Fuentes, O., Pozo, M. and Rojas, S. 1988. Kinetics of L-alanine and L-leucine transport at the blood-tissue interface in the perfused stomach. Cell. Mol. Biol. 34: 323–336.

    PubMed  CAS  Google Scholar 

  • Brenton, D.P. and Gardiner, R.M. 1988. Transport of L-phenylalanine and related amino acids at the ovine blood-brain barrier. J. Physiol. 402: 497–508.

    Google Scholar 

  • Bustamante, J.C. and Setchell, B.P. 1982. Kinetics of the facilitated diffusion of leucine into the perfused testis of the rat. J. Physiol. 324: 49P.

    Google Scholar 

  • Bustamante, J.C., Mann, G.E. and Yudilevich, D.L. 1981. Specificity of amino acid uptake at the basolateral side of the epithelium in the cat salivary gland in situ. J.Physiol 313: 65–79

    CAS  Google Scholar 

  • Bustamante, J.C., Yudilevich, D.L. and Boyd, C.A.R. 1988. A new form of asymmetry in epithelia: Kinetics of apical and basal sulphate transport in human placenta. O. 7 Exper. Physiol. 73: 1013–1016

    Google Scholar 

  • Chaumet-Riffaud, Ph., Girault, M. and Syrota, A. (1984) Characterization of muscarinic cholinergic receptors in the isolated perfused rat heart. J. Physiol. 348: 11P

    Google Scholar 

  • Crone, C. 1963. The permeability of capillaries in various organs as determined by use of the indicator diffusion method’. Acta physiol. scand. 58: 292–305.

    CAS  Google Scholar 

  • Crone, C. 1965. Facilitated transfer of glucose from blood into brain tissue. J. Physiol. 181: 103–113.

    Google Scholar 

  • Christensen, H.N. 1990. Role of amino acid transport and countertransport in nutrition and metabolism. Physiol. Rev. 70: 43–76

    PubMed  CAS  Google Scholar 

  • Christensen, H.N. 1982. Interorgan amino acid nutrition. Physiol. Rev. 62: 1193–1233

    Google Scholar 

  • Eaton, B.M. and Yudilevich, D.L. 1981. Uptake and asymmetric efflux of amino acids at maternal and fetal sides of placenta. Am. J. Physiol. 241: C106–112.

    Google Scholar 

  • Eaton, B.M., Mann, G.E. and Yudilevich, D.L. 1982. Transport specificity for neutral and basic amino acids at maternal and fetal interfaces of the guinea-pig placenta. J.Physiol. 328: 245–258.

    CAS  Google Scholar 

  • Haselton, F.R., Mueller, S.N., Howell, E.M., Levine, E.M. and Fishman, A.P. 1989. Chromatographic demonstration of reversible changes in endothelial permeability. J. Appl. Physiol. 67: 2032–2048.

    Google Scholar 

  • Hellewell, P.G. and Pearson, J.D. 1983 Kinetic analysis of prostaglandin F2 alpha and adenosine uptake in the piglet pulmonary circulation. J. Physiol. 343: 48P.

    Google Scholar 

  • Hundal, H.S., Rennie, M.J. and Watt, P.W. 1987. Characteristics of L-glutamine transport in perfused rat skeletal muscle. J. Physiol. 393: 283–305.

    PubMed  CAS  Google Scholar 

  • Hundal, H.S., Rennie, M.J. and Watt, P.W. 1989. Characteristics of acidic, basic and neutral amino acid transport in the perfused rat hindlimb. J. Physiol. 408: 93–114.

    Google Scholar 

  • Mann, G.E. and Norman, P.S.R. 1984. Regulatory effects of insulin and experimental diabetes on neutral amino acid transport in the perfused rat exocrine pancreas: Kinetics of unidirectional L-serine influx and efflux at the basolateral plasma membrane. Biochim. Biophys. Acta 778: 618–622.

    Google Scholar 

  • Mann, G.E. and Peran, S. 1986. Basolateral amino acid transport systems in the perfused exocrine pancreas: sodium-dependency and kinetic interactions between influx and efflux mechanisms. Biochim. Biophys. Acta. 858: 263–274.

    Article  PubMed  CAS  Google Scholar 

  • Mann, G.E. and Yudilevich, D.L. 1984a. Discrimination of parallel neutral amino acid transport systems in the basolateral membrane of cat salivary epithelium. J. Physiol. 347: 111127.

    Google Scholar 

  • Mann, G.E. and Yudilevich, D.L. 1984b. Rapid transcapillary exchange and unidirectional neuronal uptake of noradrenaline in the perfused rabbit heart. J. Physiol. 348: 589–600.

    Google Scholar 

  • Mann, G.E. and Yudilevich, D.L. 1987. Regulation of amino acid influx and efflux at the basolateral plasma membrane of the salivary epithelium: effects of parasympathetic nerve stimulation. J. Dental Res. 66: 569–575.

    Article  CAS  Google Scholar 

  • Mann, G.E., Pearson, J.D., Sheriff, C.-J. and Toothill, V.J. 1989. Expression of amino acid transport systems in cultured human umbilical vein endothelial cells. J. Physiol. 410: 325–339.

    Google Scholar 

  • Mann, G.E., Sheriff, C-J. and Pearson, J.D. 1990. N°-monomethyl-L-arginine and other cationic amino acids inhibit L-arginine uptake in perfused microcarrier cultures of bovine aortic endothelial cells. In: Nitric Oxide from L-Arginine: A Bioregulatory System, Eds. Moncada, S. and Higgs, E.A., pp. 331–339. Elsevier, Amsterdam.

    Google Scholar 

  • Mann, G.E., Wilson, S.M. and Yudilevich, D.L. 1984. Characteristics of a cationic amino acid transport system in the basolateral membrane of the cat salivary epithelium. J. Physiol. 351: 123–134.

    Google Scholar 

  • Mann, G.E., Zlokovic, B.V. and Yudilevich D.L. 1985. Evidence for a lactate transport system in the sarcolemmal membrane in the perfused rabbit heart: Kinetics of unidirectional influx, carrier specificity and effect of glucagon. Biochim. Biophys. Acta 819: 241248.

    Google Scholar 

  • Martin de Julian, P. and Yudilevich, D.L. 1964. A theory for the quantification of transcapillary exchange by tracer dilution curves. Am. J. Physiol. 207, 162–168.

    PubMed  CAS  Google Scholar 

  • Mepham, T.B., Overthrow, J.I., Short, A.H., and Warne, D.E. 1984. Amino acid transport interactions in lactating mammary gland of guinea-pig. J. Physiol.357 73P

    Google Scholar 

  • Norman, P.S.R. 1988. Capillary extraction of [7,8 3H]dopamine in the isolated perfused rabbit heart. J. Physiol. 407: 16P.

    Google Scholar 

  • Norman, P.S.R. and Mann, G.E. 1986. Transport characteristics of System A in the rat exocrine pancreatic epithelium analyzed using the specific non-metabolized amino acid analogue ct-methylaminoisobutyric acid. Biochim. Biophys. Acta 861: 389–394.

    Google Scholar 

  • Norman, P.S.R. and Mann, G.E. 1987. Ionic dependence of amino-acid transport in the exocrine pancreatic epithelium: calcium dependence of insulin action. J. Membr. Biol. 96: 153163.

    Google Scholar 

  • Norman, P.S.R., Habara, Y. and Mann, G.E. 1989. Paradoxical effects of endogenous and exogenous insulin on amino acid transport activity in the isolated rat pancreas: somatostatin-14 inhibits insulin action. Diabetologia 32: 177–184.

    CAS  Google Scholar 

  • Perân, S. and McGee, M.P. 1986. Unidirectional flux of phenylalanine into Vero cells. Measurement using paired tracers in perfused cultures. Biochim. Biophys. Acta 856: 231–236.

    Google Scholar 

  • Perân, S., Munoz, M. and Saiz, M.T. 1990. Rapid and steady-state amino acid transport in perfused human fibroblasts and colon adenocarcinoma cells: effects of methotrexate. Biochim. Biophys. Acta 1024: 233–240.

    Google Scholar 

  • Rennie, M.J., Idstrom, J-P., Mann, G.E., Schersten, T. and Bylund-Fellinius, A-C. 1983. A paired-tracer dilution method for characterizing membrane transport in the perfused rat hindlimb. Biochem. J. 214: 737–743.

    Google Scholar 

  • Smith, I.C.H. 1985. An interpretation of single-pass uptake curves for measuring cellular transport. In: Carrier-Mediated Transport of Solutes from Blood to Tissue. Eds. Yudilevich, D.L. and Mann, G.E., pp. 397–402. Longman, London.

    Google Scholar 

  • Sweiry, J.H. and Yudilevich, D.L. 1984. Asymmetric calcium influx and efflux at maternal and fetal sides of the guinea-pig placenta: kinetics and specificity. J. Physiol. 355: 295–311.

    Google Scholar 

  • Sweiry, J.H. and Yudilevich, D.L. 1985. Characterization of choline transport at maternal and fetal interfaces of the perfused guinea-pig placenta. J. Physiol. 366: 251–266.

    Google Scholar 

  • Sweiry, J.H. and Yudilevich, D.L. 1988. Characterization of folate uptake in guinea pig placenta. Am. J. Physiol. 254: C735–C743.

    PubMed  CAS  Google Scholar 

  • Sweiry, J.H., Page, K.R., Dacke, C.G., Abramovich, D.R. and Yudilevich, D.L. 1986. Evidence of saturable uptake mechanisms at maternal and fetal sides of the perfused human placenta by rapid paired-tracer dilution: studies with calcium and choline. J. Dev. Physiol. 8: 435–445

    Google Scholar 

  • Syrota, A., Girault, M., Pocidalo, J-J. and Yudilevich, D.L. 1982. Endothelial uptake of amino acids, sugars, lipids, and prostaglandins in rat lung. Am. J. Physiol. 243: C20–C26.

    Google Scholar 

  • Taylor, P.M. and Rennie, M.J. 1987. Perivenous localisation of Na-dependent glutamate transport in perfused rat liver. FEBS Lett 221: 370–374.

    CAS  Google Scholar 

  • Taylor, P.M. and Rennie, M.J. 1988. Amino acid fluxes across sinusoidal membranes of the perfused rat liver: relationships with portal ammonia concentration. In: Advances in Ammonia Metabolism and Hepatic Encephalopathy. Eds. Soelers, P.B., Wilson, J.H.P, Meyer, A.J., Holm, E. Amsterdam: Elsevier, pp, 45–52.

    Google Scholar 

  • Taylor, P.M., Egan, C.J. and Rennie, M.J. 1989. Transport of glutamine across blood-facing membranes of perfused rat jejunum. Am. J. Physiol. 256: E550–E558.

    Google Scholar 

  • Toothill, V.J., Eaton, B.M., Davis, H., Pearson, J.D. and Mann, G.E. Solute permeability of endothelial cell junctions in perfused microcarrier cultures of human umbilical vein endothelial cells. Int. J. Microcir. Clin. Exp. (abstract in press).

    Google Scholar 

  • Wangler, R.D., Gorman, M.W., Wang, C.Y., DeWitt, D.F., Chan, I.S., Bassingthwaighte, J.B. and Sparks, H.V. 1989. Transcapillary adenosine transport and interstitial adenosine concentration in guinea pig hearts. Am. J Physiol. 257: H89–106.

    PubMed  CAS  Google Scholar 

  • Watt, P.W., MacLennan, P.A., Hundal, H.S., Kuret, C.M. and Rennie, M.J. 1988. L(+)-Lactate transport in perfused rat skeletal muscle: kinetic characteristics and sensitivity to pH and transport inhibitors. Biochim. Biophys. Acta 944: 213–222.

    Google Scholar 

  • Wheeler, C.P.D. and Yudilevich, D.L. 1988. Transport and metabolism of adenosine in the perfused guinea-pig placenta. J. Physiol. 405: 511–526.

    Google Scholar 

  • Wheeler, C.P.D. and Yudilevich, D.L. 1989a. Effect of insulin, prostaglandin El and uptake inhibitors on glucose transport in the perfused guinea-pig placenta. J. Dev. Physiol. 11: 159–169.

    Google Scholar 

  • Wheeler, C.P.D. and Yudilevich, D.L. 1989b. Lysine and alanine transport in the perfused guinea-pig placenta. Biochim. Biophys. Acta 978: 257–266.

    Google Scholar 

  • Yudilevich, D.L. 1985. Characterisation of membrane carriers and receptors at the blood-tissue interface by single circulation paired-tracer dilution. In: Carrier mediated transport of solutes from Blood to Tissue. Eds. Yudilevich, D.L. and Mann, G.E., pp. 1–31. Longman, London.

    Google Scholar 

  • Yudilevich, D.L. 1989. Blood-tissue transport of substrates in the heart: studies by single circulation tracer dilution. Int. J. Microcirc. Clin.Exp. 8: 397–409.

    Google Scholar 

  • Yudilevich, D.L. and Barros, L.F. 1990. Transport of amino acids and nucleosides in the placenta. Biochem. Soc. Transactions 14: 1146–1150

    Google Scholar 

  • Yudilevich, D.L. and Eaton, B.M. 1980. Amino acid carriers at the maternal and fetal surfaces of placenta by single circulation paired-tracer dilution. Kinetics of phenylalanine transport. Biochim. Biophys. Acta 596: 315–319

    Article  PubMed  CAS  Google Scholar 

  • Yudilevich, D.L. and Mann, G.E. 1982. Unidirectional uptake of substrates at the blood-side of secretory epithelia: stomach, salivary gland, pancreas. Fed. Proc. 41: 3045–3053.

    Google Scholar 

  • Yudilevich, D.L. and Mann, G.E. (eds.) 1985. Carrier-mediated Transport of Solutes from Blood to Tissue. Longman, London.

    Google Scholar 

  • Yudilevich, D.L. and Sweiry, J.H. 1985. Transport of amino acids in the placenta. Biochim. Biophys. Acta 822: 169–201.

    Google Scholar 

  • Yudilevich, D.L. and Wheeler, C.D.P. 1988. Placental transport: the brush border and the basal sides of the trophoblast, and a role for the fetal endothelium?. In: Fetal and Neonatal Development. Ed. Jones, C.T., pp. 47–57. Perinatology Press, New York.

    Google Scholar 

  • Yudilevich, D.L., de Rose, N. and Sepulveda, F.V. 1972. Facilitated transport of amino acid through the blood-brain barrier of the dog studied in a single capillary circulation. Brain Res 44: 569–578.

    CAS  Google Scholar 

  • Yudilevich, D.L., Eaton, B.M., Short, A.H. and Leichtweiss, H.-P. 1979. Glucose carriers at maternal and fetal sides of the trophoblast in guinea-pig placenta. Am. J. Physiol. C205–212.

    Google Scholar 

  • Zlokovic, B.V., Segal, M.B., Begley, D.J., Dayson, H. and Rakic, L. 1985. Permeability of the blood-cerebrospinal fluid and blood-brain barriers to thyrotropin-releasing hormone. Brain Research 358: 191–199.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Physiology, Biomedical Sciences Division, King’s College, University of London, Campden Hill Road, London, W8 7AH, UK

    David L. Yudilevich, Luis A. Sobrevía & L. Felipe Barros

  2. Departamento de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla, 70055, Santiago, Chile

    David L. Yudilevich, Luis A. Sobrevía & L. Felipe Barros

Authors
  1. David L. Yudilevich
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luis A. Sobrevía
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Felipe Barros
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of London, London, UK

    David L. Yudilevich

  2. University of Chile, Santiago, Chile

    Rosa Devés

  3. University of Málaga, Málaga, Spain

    Salvador Perán

  4. The Hebrew University of Jerusalem, Jerusalem, Israel

    Z. Ioav Cabantchik

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Yudilevich, D.L., Sobrevía, L.A., Barros, L.F. (1991). Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired-Tracer Dilution: A Review of Recent Studies. In: Yudilevich, D.L., Devés, R., Perán, S., Cabantchik, Z.I. (eds) Cell Membrane Transport. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9601-8_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-4757-9601-8_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9603-2

  • Online ISBN: 978-1-4757-9601-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation