Abstract
Fluorescence measurements are among the most powerful methods for investigating structure and structural changes on cell surfaces. The basic strength of this method is that nanomolar concentrations of fluorophores can be detected in the presence of a high level of background noise provided by the cells. An ideal fluorescent probe absorbs and emits at wavelengths not in common with the cellular components, has a high quantum yield, and can be placed specifically into a location such that it is sensitive to the structural aspect of interest. Recently there has been a great expansion in the commercial availability of fluorescent probes with a broad range of fluorescent properties and reactive groups for conjugation (e.g., Haugland, 1989). The requirement for specific placement on a macromolecular/cellular complex remains the most challenging experimentally. In this regard, specific ligands and specific monoclonal antibodies that can be fluorescently modified are valuable reagents. In this chapter we describe our use of quantitative fluorescence measurements to investigate the binding properties of cell surface receptors.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Baird, B., and Holowka, D., 1988, in Spectroscopic Membrane Probes (L. Loew, ed.), CRC Press, Boca Raton, pp. 93–116.
Baird, B., Erickson, J., Goldstein, B., Kane, P., Menon, A. K., Robertson, D., and Holowka, D., 1988, in Theoretical Immunology (A. Perelson, ed.), Addison-Wesley, Reading, Mass., pp. 41–59.
Barsumian, E. L., Isersky, C., Petrino, M. G., and Siraganian, R. P., 1981, Eur. J. Immunol. 11:317–323.
Berg, H. C., and Purcell, E. M., 1977, Biophys. J. 20:193–219.
Carson, D., and Metzger, H., 1974, Immunochemistry 11:355–359.
Dembo, M., and Goldstein, B., 1978a, Immunochemistry 15:307–313.
Dembo, M., and Goldstein, B., 1978b, J. Immunol. 121:345–353.
Dembo, M., Goldstein, B., Sobotka, A. K., and Lichtenstein, L. M., 1979, J. Immunol. 123:1864–1871.
Eisen, H., and McGuigan, J., 1968, in Methods in Immunology and Immunochemistry (W. C. Williams and M. Chase, eds.), Academic Press, New York.
Erickson, J. W., 1988, Equilibrium and kinetic studies of a model ligand-receptor system: Monovalent and bivalent ligand interactions with immunoglobulin E, Ph.D. thesis, Cornell University.
Erickson, J., Kane, P., Goldstein, B., Holowka, D., and Baird, B., 1986, Mol. Immunol. 23:769–780.
Erickson, J., Goldstein, B., Holowka, D., and Baird, B., 1987, Biophys. J. 52:657–662.
Estes, K., Monfalcone, L., Hammes, S., Holowka, D., and Baird, B., 1987, J. Cell Biol. 105:747–755.
Fewtrell, C., 1985, in Calcium in Biological Systems (G. Weiss, J. Putney, and R. Rubin, eds.), Plenum Press, New York, pp. 129–136.
Fewtrell, C., Kessler, A., and Metzger, H., 1979, Adv. Inflam. Res. 1:205–221.
Flory, P. J., 1953, Principles of Polymer Chemistry, Cornell University Press, Ithaca, N.Y.
Goldstein, B., Posner, R. G., Torney, D. C., Erickson, J., Holowka, D., and Baird, B., 1989, Biophys. J. 56:955–966.
Haugland, R. P., 1989, Molecular Probes Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Eugene, Ore.
Holowka, D., and Baird, B., 1983, Biochemistry 22:3466–3474.
Ishizaka, T., Hirata, F., Ishizaka, K., and Axelrod, J., 1981, in Biochemistry of the Acute Allergic Reactions (E. Becker, A. Simon, and K. Austen, eds.), Liss, New York, pp. 213–227.
Isersky, C., Rivera, J., Mims, S., and Triche, T., 1979, J. Immunol. 122:1926–1936.
Kane, P., Erickson, J., Fewtrell, C., Baird, B., and Holowka, D., 1986, Mol. Immunol. 23:783–790.
Kane, P., Holowka, D., and Baird, B., 1988, J. Cell Biol. 107:969–980.
Liu, F. T., Bohn, J. W., Ferry, E. L., Yamamoto, H., Molinaro, C. A., Sherman, L. A., Klinman, N. R., and Katz, D., 1980, J. Immunol. 124:2728–2735.
Menon, A. K., Holowka, D., and Baird, B., 1984, J. Cell Biol. 9:577–583.
Menon, A. K., Holowka, D., Webb, W. W., and Baird, B., 1986a, J. Cell Biol. 102:534–540.
Menon, A. K., Holowka, D., Webb, W. W., and Baird, B., 1986b, J. Cell Biol. 102:541–550.
Metzger, H., Alcaraz, G., Hohman, R., Kinet, J.-P., Pribluda, V., and Quarto, R., 1986, Annu. Rev. Immunol. 4:419–470.
Pecht, I., and Lancet, D., 1977, in Chemical Relaxation in Molecular Biology (I. Pecht and R. Rigler, eds.), Springer-Verlag, Berlin, pp. 306–338.
Perelson, A., and DeLisi, C., 1980, Math. Biosci. 48:71–110.
Robertson, D., Holowka, D., and Baird, B., 1986, J. Immunol. 136:4565–4572.
Schweitzer-Stenner, R., Light, A., Luscher, I., and Pecht, I., 1987, Biochemistry 26:3602–3612.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Erickson, J., Posner, R., Goldstein, B., Holowka, D., Baird, B. (1991). Analysis of Ligand Binding and Cross-Linking of Receptors in Solution and on Cell Surfaces. In: Dewey, T.G. (eds) Biophysical and Biochemical Aspects of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9513-4_6
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9513-4_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9515-8
Online ISBN: 978-1-4757-9513-4
eBook Packages: Springer Book Archive