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Abstract

A number of articles and books have appeared in recent years describing in varying detail the theory and application of time-resolved fluorescence spectroscopy in the chemical and biological sciences (Badea and Brand, 1979; Demas, 1983; Lakowicz, 1983; Cundall and Dale, 1983; Gratton et al., 1984a; Jameson et al., 1984; Taylor et al., 1987; Jameson and Reinhart, 1989). Our goal in this chapter is thus not to treat the subject exhaustively but rather to reiterate the fundamental considerations and to provide the reader with a feeling for the practical aspects that must be apprehended before time-resolved methods can be profitably applied to complex biological systems.

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References

  • Alcala, J. R., Gratton, E., and Jameson, D. M., 1985, Anal. Instrum. 14:225–250.

    Article  CAS  Google Scholar 

  • Alcala, J. R., Gratton, E., and Prendergast, F. G., 1987, Biophys. J. 51:587–596.

    Article  PubMed  CAS  Google Scholar 

  • Badea, M. G., and Brand, L., 1979, Methods Enzymol. 61:378–425.

    Article  PubMed  CAS  Google Scholar 

  • Barbieri, B. F., DePiccoli, F., and Gratton, E., 1988, Proc. S.P.I.E. 909:366–369.

    CAS  Google Scholar 

  • Beechem, J. M., and Brand, L., 1985, Annu. Rev. Biochem. 54:43–71.

    Article  PubMed  CAS  Google Scholar 

  • Beechem, J. M., and Gratton, E., 1988, Proc. S.P.I.E. 909:70–81.

    CAS  Google Scholar 

  • Beechem, J. M., Knutson, J. R., Ross, J. B. A., Turner, B. W., and Brand, L., 1983, Biochemistry 22:6054–6058.

    Article  CAS  Google Scholar 

  • Beechem, J. M., Knutson, J. R., and Brand, J. R., 1986, Biochem. Soc. Trans. 14:832–835.

    PubMed  CAS  Google Scholar 

  • Belford, C. G., Belford, R. L., and Weber, G., 1972, Proc. Natl. Acad. Sci. USA 69:1392–1393.

    Article  PubMed  CAS  Google Scholar 

  • Bevington, P. R. (ed.), 1969, Data Reduction and Error Analysis in the Physical Sciences, McGraw-Hill, New York.

    Google Scholar 

  • Birks, J. B., and Munro, I. H., 1967, Prog. React. Kinet. 4:239–303.

    CAS  Google Scholar 

  • Brand, L., Knutson, J. R., Davenport, L., Beechem, J. M., Dale, R. E., Walbridge, D. G., and Kowalczyk, A. A., 1985, in Spectroscopy and the Dynamics of Molecular Biological Systems, P. M. Bayley and R. C. Dole eds. Academic Press, London.

    Google Scholar 

  • Bucci, E., and Steiner, R. F., 1988, Biophys. Chem. 30:199–224.

    Article  PubMed  CAS  Google Scholar 

  • Cohn, E. L., and Edsall, J. T., 1943, in Proteins, Amino Acids and Peptides as Ions and Dipolar Ions, Reinhold, New York.

    Google Scholar 

  • Cundall, R. B., and Dale, R. E. (eds.), 1983, Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology, Plenum Press, New York.

    Google Scholar 

  • Demas, J. N., 1983, Excited State Lifetime Measurements, Academic Press, New York.

    Google Scholar 

  • Duschinsky, F., 1933, Z. Phys. 81:7–22.

    Article  CAS  Google Scholar 

  • Eftink, M. R., and Wasylewski, Z., 1989, Biochemistry 28:382–391.

    Article  PubMed  CAS  Google Scholar 

  • Fiorini, R. M., Valentino, M., Glaser, M., Gratton, E., and Curatola, G., 1988, Biochim. Biophys. Acta 939:482–485.

    Google Scholar 

  • Fizeau, A., 1849, C.R. Acad. Sci. 31:90–93.

    Google Scholar 

  • Gaviola, E., 1926, Ann. Phys. (Leipzig) 81:681–710.

    CAS  Google Scholar 

  • Gratton, E., and Barbieri, B., 1986, Spectroscopy 1:28–36.

    CAS  Google Scholar 

  • Gratton, E., and Jameson, D. M., 1985, Anal. Chem. 57:1694–1697.

    Article  CAS  Google Scholar 

  • Gratton, E., and Limkeman, M., 1983, Biophys. J. 44:315–324.

    Article  PubMed  CAS  Google Scholar 

  • Gratton, E., Jameson, D. M., and Hall, R. D., 1984a, Annu. Rev. Biophys. Bioeng. 13:105–124.

    Article  PubMed  CAS  Google Scholar 

  • Gratton, E., Jameson, D. M., Rosato, N., and Weber, G., 1984b, Rev. Sci. Instrum. 55:486–494.

    Article  CAS  Google Scholar 

  • Hazlett, T. L., and Jameson, D. M., 1988, Proc. S.P.I.E. 909:412–419.

    CAS  Google Scholar 

  • Hazlett, T. L., Johnson, A. E., and Jameson, D. M., 1989, Biochemistry 28:4109–4117.

    Article  PubMed  CAS  Google Scholar 

  • Jameson, D. M., and Gratton, E., 1983, in New Directions in Molecular Luminescence (D. Eastwood, ed.), ASTM STP 4822, American Society for Testing and Materials, Philadelphia, p. 67.

    Chapter  Google Scholar 

  • Jameson, D. M., and Reinhart, G. D. (eds.), 1989, Fluorescent Biomolecules: Methodologies and Applications, Plenum Press, New York.

    Google Scholar 

  • Jameson, D. M., Weber, G., Spencer, R. D., and Mitchell, G., 1978, Rev. Sci. Instrum. 49:510–514.

    Article  PubMed  CAS  Google Scholar 

  • Jameson, D. M., Gratton, E., and Hall, R. D., 1984, Appl. Spectrosc. Rev. 20:55–106.

    Article  CAS  Google Scholar 

  • Jameson, D. M., Gratton, E., and Eccleston, J. F., 1987, Biochemistry 26:3894–3901.

    Article  PubMed  CAS  Google Scholar 

  • Kinosita, K., Jr., Kawato, S., and Ikegami, A., 1977, Biophys. J. 20:289–305.

    Article  PubMed  CAS  Google Scholar 

  • Knutson, J. R., Beechem, J. M., and Brand, L., 1983, Chem. Phys. Lett. 102:501–507.

    Article  CAS  Google Scholar 

  • Lakowicz, J. R., 1983, Principles of Fluorescence Spectroscopy, Plenum Press, New York.

    Book  Google Scholar 

  • Lakowicz, J. R., Cherek, H., and Maliwal, B. P., 1985, Biochemistry 24:376–383.

    Article  PubMed  CAS  Google Scholar 

  • Laws, W. R., and Jameson, D. M., 1989, in Synchrotron Radiation in Structural Biology (R. M. Sweet, ed.), Plenum Press, New York.

    Google Scholar 

  • Lipari, G., and Szabo, A., 1980, Biophys. J. 30:489–506.

    Article  PubMed  CAS  Google Scholar 

  • McGown, L. B., and Bright, F. V., 1984, Anal. Chem. 56:2195–2199.

    Article  Google Scholar 

  • McMeekin, T. L., Groves, M. L., and Hipp, N. J., 1949, J. Am. Chem. Soc. 7:3298–3300.

    Article  Google Scholar 

  • Neal, S. E., Eccleston, J. F., Hall, A., and Webb, M. R., 1988, J. Biol. Chem. 263:19718–19722.

    PubMed  CAS  Google Scholar 

  • Nordlund, T. M., 1988, Proc. S.P.I.E. 909:35–50.

    CAS  Google Scholar 

  • Perrin, F., 1934, J. Phys. Radium 5:487–511.

    Article  Google Scholar 

  • Small, E. W., Libertini, L. J., and Small, J. R., 1988, Proc. S.P.I.E. 909:97–107.

    CAS  Google Scholar 

  • Spencer, R. D., and Weber, G., 1969, Ann. N.Y. Acad. Sci. 158:361–376.

    Article  CAS  Google Scholar 

  • Spencer, R. D., and Weber, G., 1970, J. Chem. Phys. 52:1654–1663.

    Article  CAS  Google Scholar 

  • Szmacinski, H., Jayaweera, R., Cherek, H., and Lakowicz, J. R., 1987, Biophys. Chem. 27:233–241.

    Article  PubMed  CAS  Google Scholar 

  • Taylor, D. L., Waggoner, A. S., Lanni, F., Murphy, R. F., and Birge, R. R. (eds.), 1987, Applications of Fluorescence in the Biomedical Sciences, Liss, New York.

    Google Scholar 

  • Teale, F. W. J., 1983, in Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology (R. B. Cundall and R. E. Dale, eds.), Plenum Press, New York, p. 59.

    Chapter  Google Scholar 

  • Valat, P., Reinhart, G. D., and Jameson, D. M., 1988, Photochem. Photobiol. 47:787–790.

    Article  PubMed  CAS  Google Scholar 

  • Veselova, T. V., Limareva, L. A., and Cherkasov, A. S., 1965, Izv. Akad. Nauk SSSR Ser. Fiz. 29:1340–1345.

    CAS  Google Scholar 

  • Veselova, T. V., Cherkasov, A. S., and Shirokov, A. S., 1970, Opt. Spectrosc. 29:617–618.

    Google Scholar 

  • Wallach, D., 1967, J. Chem. Phys. 47:5258–5268.

    Article  CAS  Google Scholar 

  • Weber, G., 1952, Biochem. J. 51:145–155.

    PubMed  CAS  Google Scholar 

  • Weber, G., 1966, in Fluorescence and Phosphorescence Analysis (D. M. Hercules, ed.), Interscience, New York, pp. 217–240.

    Google Scholar 

  • Weber, G., 1956, J. Opt. Sci. Amer. 46:646–655.

    Google Scholar 

  • Weber, G., 1977, J. Chem. Phys. 66:4081–4091.

    Article  CAS  Google Scholar 

  • Weber, G., 1978, Acta Phys. Pol. A 54:859–865.

    Google Scholar 

  • Weber, G., 1981, J. Phys. Chem. 85:949–953.

    Article  CAS  Google Scholar 

  • Williams, B. W., and Stubbs, C. D., 1988, Biochemistry 27:7994–7999.

    Article  PubMed  CAS  Google Scholar 

  • Yguerabide, J., 1972, Methods Enzymol. 26:498–578.

    Article  PubMed  CAS  Google Scholar 

  • Yguerabide, J., and Yguerabide, E. E., 1984, in Optical Techniques in Biological Research (D. L. Rousseau, ed.), Academic Press, New York.

    Google Scholar 

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Jameson, D.M., Hazlett, T.L. (1991). Time-Resolved Fluorescence in Biology and Biochemistry. In: Dewey, T.G. (eds) Biophysical and Biochemical Aspects of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9513-4_4

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  • DOI: https://doi.org/10.1007/978-1-4757-9513-4_4

  • Publisher Name: Springer, Boston, MA

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