Résumé
La comparaison de structures des oligosaccharides attachés à plusieurs protéines obtenues du fluide cérébrospinale et du sérum humains a montrée des différences frappantes (“brain-type” versus “serum-type” glycosylation) qui ont été attribuées aux alentours differents de ces deux fluides. On n’a pas rencontré une modification des oligosaccharides pendant le passage au-delà de la barrière hémato-encephalique. La recherche de la glycosylation de protéines cérébrospinales permet alors d’obtenir des informations sur le lieu de biosynthèse (dans le cerveau ou ailleurs dans le corps) des protéines particulières en question.
Summary
Comparison of the patterns of N-linked oligosaccharides attached to several proteins isolated from human cerebrospinal fluid and serum reveals striking differences (“brain-type” versus “serum-type” glycosylation) which are attributed to the different physiological surroundings of both body fluids. No modification of oligosaccharides was found to occur during passage of proteins across the blood-brain barrier. Glycosylation analysis of CSF-proteins can thus be used to gain information on the site of biosynthesis (i.e. systemic or intrathecal) of specific proteins in question.
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Hoffmann, A., Grabenhorst, E., Nimtz, M., Conradt, H.S. (1996). “Brain-Tissue-Specific” Versus “Serum-Specific” Posttranslational Modification of Human Cerebrospinal Fluid Polypeptides with N-Linked Carbohydrates. In: Couraud, PO., Scherman, D. (eds) Biology and Physiology of the Blood-Brain Barrier. Advances in Behavioral Biology, vol 46. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9489-2_18
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DOI: https://doi.org/10.1007/978-1-4757-9489-2_18
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