Abstract
Coriolus versicolor, a white-rot basidiomycete, elaborates cellulolytic and ligninolytic enzymes both in situ and in vitro (Evans and Palmer, 1983; Taylor et al., 1987, 1988; Moore et al., 1989). These enzymes can degrade cellulose (Peterson et al., 1963) and lignin (Harvey et al., 1987), the main polymers of wood (Higuchi, 1985). Besides its presence within wood, lignocellulose often constitutes an unwanted component in the paper-pulp industry and also renders certain agricultural commodities less digestible to ruminants (Dodson et al., 1986; Van der Meer et al., 1987). Thus, an available supply of ligno-cellulolytic enzymes could be of marked industrial value. To maintain an adequate supply, substrate induction coupled to hyphal ‘batch culture’ (Fahraeus and Reinhammer, 1967) and/or genetic engineering techniques (Maniatis et al., 1982; Bennett and Lasure, 1985; Current Protocols in Molecular Biology, 1987) are being sought to over-produce these enzymes. Here are reported pertinent recombinant DNA techniques (Williams et al., 1988, 1989a, b) which may result in enhanced enzyme synthesis by Escherichia coli transformants harboring the C. versicolor gene(s) for polyphenol oxidase (PPO), an enzyme relevant to lignin degradation (Kirk and Kelman, 1965) as well as converting the purported disease-resistance, phenolic compounds (Taylor et al., 1989) to diquinones (Fric, 1976).
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References
Bennett, J.W. and Lasure, L.L. (1985). Genetic Manipulations in Fungi. Academic Press, NY.
Current Protocols in Molecular Biology. (1987). John Wiley and Sons, NY.
Dodson, P.A., Harvey, P.J., Evans, C.S. and Palmer, J.M. (1986). In: Biotechnology in the Pulp and Paper Industry p. 185. 3rd International Conference, Stockholm, Sweden.
Evans, C.S. and Palmer, J.M. (1983). Ligninolytic activity of Coriolus versicolor. J. Gen. Microbiol. 129, 2103–2108.
Fahraeus, G. and Reinhammer, B. (1967). Large scale production and purification of laccase from culture of the fungus Polyporus versicolor and some properties of lacease. Acta Chem. Scand. 21, 2367–2378.
Fric, F. (1976). Oxidative enzymes. In: Physiological Plant Pathology. (R. Hertefus and P.H. Williams, eds.), pp. 617–631. Springer-Verlag, Heidelberg.
Harvey, P.J., Schoemaker, H.E. and Palmer, J.M. (1987). Lignin degradation by white fungi. Plant Cell Environ. 10, 709–714.
Higuchi, T. (1985). Biosynthesis and Biodegradation of Wood Components, pp. 704, Academic Press, NY.
Kirk, K.T. and Kelman, A. (1965). Lignin degradation as related to polyphenol oxidases of selected wood decaying basidiomycetes. Phytopathol. 55, 739–745.
Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Moore, N.L., Mariam, D.H., Williams, A.L. and Dashek, W.V. (1989). Substrate specificity, de novo synthesis and partial purification of polyphenol oxidase from the wood-decay fungus, Coriolus versicolor. J. Indust. Microbiol. 4 (in press).
Peterson, G., Cowling, E.B. and Porath, J. (1963). Studies on cellulolytic enzymes. I. Isolation of a low molecular-weight cellulose from Polyporous versicolor. Biochem. Biophys. Acta 67, 1–8.
Taylor, R., Llewellyn, G.C. Mayfield, J.E. Shortle, W.C. and Dashek, W.V. (1987). Time-dependent appearance of extracellular polyphenol oxidase in relation to the bimodal growth response of Coriolus versicolor. In: Biodeterioration Research I. (G.C. Llewellyn and C.E. O’Rear, eds.), pp. 63–74, Plenum Press, NY.
Taylor, R., Dashek, W.V., Williams, A.L., Llewellyn, G.G., Shortle, W.C. and Mayfield, J.E. (1988). Ultrastructure of Coriolus versicolor in relation to a catechol-induced bimodal growth response. International Biodeterioration 24, 343–358.
Taylor, R., Llewellyn, G.C. O’Rear, C.E., Mayfield, J.E., Smith, K., Williams, A.L. and Dashek, W.V. (1989). In vitro growth of Coriolus versicolor, a wood-decay fungus, responds differentially to catechol and tannic acid. In: Biodeterioration Research II. (G.C. Llewellyn and C.E. O’Rear, eds.), pp. 451–464, Plenum Press, NY.
Van der Meer, J.M., Rijkers, B.A. and Feuanti, M.P. (1987). Degradation of Lignocellulosics in Ruminants and in Industrial Processes. Elsevier, Amsterdam.
Williams, A.C., Moore, N.L., Dashek, W.V. and Williams, A.L. (1988). Genetic engineering of Coriolus versicolor’s polyphenol oxidase gene(s) and its industrial applications. USDA Symposium in Biotechnology, Alabama Agricultural & Mechanical University, Huntsville, AL (A-9).
Williams, A.C., Moore, N.L., Dashek, W.V. and Williams, A.L. (1989a). Biotechnology of the gene(s) for polyphenol oxidase from the wood-decay fungus, Coriolus versicolor. ASM Conference in Biotechnology, p. 17, Orlando, FL.
Williams, A.C., Moore, N.L., Dashek, W.V. and Williams, A.L. (1989b). Genetic engineering of the gene(s) for polyphenol oxidase from the wood decay fungus, Coriolus versicolor. Society of Industrial Microbiology News (in press).
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Williams, A.C., Moore, N.L., Dashek, W.V., Williams, A.L. (1990). Coriolus versicolor, A Model System to Investigate the Biotechnology of Wood-Deteriorating Enzymes. In: Llewellyn, G.C., O’Rear, C.E. (eds) Biodeterioration Research. Biodeterioration Research, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9453-3_32
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