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LC-MS and LC-MS-MS for Biomedical Analyses

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Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((MSBA,volume 18 A))

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Abstract

The identification of components separated by LC* can be done either by preparative chromatography and off-line MS or, with advantages, by on-line LC-MS.θ The moving belt and DLI are the oldest commercially available interfaces. The belt allows EI, CI or FAB to be chosen for ionisation, but samples may suffer from thermal degradation. DLI is limited to CI and suits thermolabile samples, but the 5 μm orifice in the interface is easily blocked. DLI has been replaced by thermospray LC-MS, which has become less elaborate and is now available in nearly all spectrometers on the market.

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Abbreviations

LC:

liquid chromatography (HPLC)

MS:

mass spectrometry

API:

atmospheric pressure ionization

CI:

chemical ionization

DLI:

direct liquid introduction

EI:

electron impact

FAB:

fast atom bombardment

SIM:

selected ion monitoring.

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Author information

Authors and Affiliations

  1. Department of Pharmacy, State University, A. Deusinglaan 2, 9713 AW, Groningen, The Netherlands

    A. P. Bruins

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  1. A. P. Bruins
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Editor information

Editors and Affiliations

  1. Guildford Academic Associates, Guildford, UK

    Eric Reid

  2. Hoechst UK, Milton Keynes, UK

    J. D. Robinson

  3. ICI Pharmaceuticals Division, Macclesfield, UK

    Ian D. Wilson

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© 1988 Springer Science+Business Media New York

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Bruins, A.P. (1988). LC-MS and LC-MS-MS for Biomedical Analyses. In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_48

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  • DOI: https://doi.org/10.1007/978-1-4757-9424-3_48

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-9426-7

  • Online ISBN: 978-1-4757-9424-3

  • eBook Packages: Springer Book Archive

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