Abstract
In analytical chemistry HPLC techniques are notably powerful, important and widely used, yet they are still viewed as ‘black magic’ by many practitioners because of a lack of basic understanding of the Chromatographic process. Investigations of retention mechanisms have as their goal a complete and exact understanding of retention, accounting for influences of solute type, mobile-phase composition and stationary-phase structure. This would allow separations to be pre-calculated, largely eliminating trial-and-error development. It would also be feasible to devise a rigorously correct retention index system for HPLC, to obtain exact physicochemical information, and to develop a ‘truly expert’ system.
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© 1988 Springer Science+Business Media New York
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Dorsey, J.G. (1988). Retention Mechanisms in Reversed-Phase HPLC. In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_45
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DOI: https://doi.org/10.1007/978-1-4757-9424-3_45
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