Abstract
AI* agents in biological fluids are commonly analyzed chromatographically, maybe by GC but usually by HPLC — typically RP — with UV detection (sometimes fluorescence or EC). HPLC is also convenient in percutaneous absorption studies on AI agents in gels and creams. Solvent extraction is often not necessary if the drug concentration, e.g. in plasma, is relatively high: precipitation of plasma proteins is often sufficient, provided that a pre-column is used.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- AI:
-
anti-inflammatory
- EC:
-
electrochemical
- Fa:
-
fluocinolone acetonide
- i.s.:
-
internal standard
- r:
-
correlation coefficient
- RP:
-
reversed phase
- SDS:
-
sodium dodecyl sulphate
References
Rance, M.J., Jordan, B.J. & Nichols, J.D. (1975) J. Pharm. Pharmacol. 27, 425–429.
Moffat, A.C., Jackson, J.V., Moss, M.S. & Widdop, B., eds. (1986) Clarke’s Isolation and Identification of Drugs, 2nd edn., The Pharmaceutical Press, London.
McElnay, J.C. & Nicol, A.C. (1984) Int. J. Pharm. 19, 89–96.
McElnay, J.C, Taggart, A.J., Kerr, B. & Passmore, P. (1986) Int. J. Pharm. 33, 195–199.
Skellern, G.G. & Salole, E.G. (1975) J. Chromatog. 114, 483–485.
Shaffer, J.A., Williams, S.E., Turnberg, L.A., Houston, J.B. & Rowland, M. (1983) Gut 24, 182–186.
Olivesi, A. (1985) Therapie 40, 5–7.
Hayes, M., Alam, A.F., Bruckner, F.E., Doherty, S.M., Myles, A., English, J., Marks, V. & Chakraborty, J. (1983) Annals Rheum. Dis. 42, 151–154.
McCann, J.P., McElnay, J.C., Nicholls, D.P., Scott, M.G. & Stanford, CF. (1987) Br. J. Clin. Pharmacol. 23, 652P.
Delargy, H. (1981) Ph.D. Thesis, Dept. of Pharmacy, The Queen’s University of Belfast.
Rose, J.Q. & Jusko, W.J. (1979) J. Chromatog. 162, 273–280.
McElnay, J.C., Mukhtar, H.A., D’Arcy, P.F. & Temple, D.T. (1982) J. Trop. Med. Hyg. 85, 153–158 (& see 159-163).
Yahya, A.M., McElnay, J.C. & D’Arcy, P.F. (1985) Int. J. Pharm. 25, 217–223.
Yahya, A.M., McElnay, J.C. & D’Arcy, P.F. (1986) Int. J. Pharm. 34, 137–143.
McElnay, J.C, Sidahmed, A.M., D’Arcy, P.F. & McQuade, R.D. (1985) Int. J. Pharm. 26, 267–274.
Bergqvist, Y. & Olin, A. (1982) Acta Pharmaceutica Suecica 19, 161–174.
Alvan, G., Ekman, L. & Lindstrom, B. (1982) J. Chromatog. 229, 241–247.
Staiger, M.A., Nguyen-Dinh, P. & Churchill, F.C. (1981) J. Chromatog. 225, 139–149.
Anderson, K. & Larsson, H. (1974) Arzneim.-Forsch./Drug Res. 24, 1686–1688.
Giacalone, E. & Valzelli, L. (1966) Med. Pharmacol. Exp. 16, 102–106.
Catanesse, B., Lagana, A., Marino, A., Picollo, R. & Rotatori, M. (1986) Pharmacol. Res. Comm. 18, 385–403.
Benson, H.A.E. & McElnay, J.C. (1987) J. Chromatog. 394, 395–399.
Skauen, D.M. & Zentner, G.M. (1984) Int. J. Pharm. 20, 234–235.
McElnay, J.C., Kennedy, T.A. & Harland, R. (1987) Int. J. Pharm. 40, 105–110.
Giese, R.W. (1983) Clin. Chem. 29, 1331–1343.
Drayer, D.E. (1984) in Proceedings of the Second World Conference on Clinical Pharmacology and Therapeutics (Lemberger, L. & Reidenberg, M., eds.), Am. Soc. for Pharmacology & Experimental Therapeutics, Bethesda, MD., pp. 809–819.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer Science+Business Media New York
About this chapter
Cite this chapter
McElnay, J.C. (1988). HPLC Analysis of Anti-Inflammatory Agents (Non-Steroidal and Steroidal). In: Reid, E., Robinson, J.D., Wilson, I.D. (eds) Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular. Methodological Surveys in Biochemistry and Analysis, vol 18 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9424-3_11
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9424-3_11
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9426-7
Online ISBN: 978-1-4757-9424-3
eBook Packages: Springer Book Archive