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Bacterial Growth and Lysis pp 105–117Cite as

Crystalline Bacterial Cell Surface Layers and their Application Potentials

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Part of the book series: Federation of European Microbiological Societies Symposium Series ((FEMS,volume 65))

Abstract

Crystalline cell surface layers (S-layers) have been identified as the outermost envelope component in hundreds of different species of every taxonomical group of walled eubacteria and archaebacteria (Fig. 1) by electron microscopy of either freeze-etched, freeze-dried and shadowed, negatively stained, or thin sectioned preparations (for reviews see Sleytr, 1978; Sleytr and Messner, 1983, 1988 a; Sleytr et al., 1988; Messner and Sleytr, 1992).

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References

  • Altman, E., Brisson, J.-R., Gagné, S.M., Kolbe, J., Messner, P. and Sleytr, U.B. (1992) Structure of the glycan chain from the surface layer glycoprotein of Clostridium thermohydrosulfuricum L77-66. Biochim. Biophys. Acta, in press.

    Google Scholar 

  • Baumeister, W. and Engelhardt, H. (1987) Three-dimensional structure of bacterial surface layers, in “Electron Microscopy of Proteins” vol. 6, “Membraneous Structure” (Harris, J.R. and Horne, R.W., Eds.), pp. 109–154. Academic Press, London.

    Google Scholar 

  • Baumeister, W., Wildhaber, I. and Phipps, B.M. (1989) Principles of organization in eubacterial and archaebacterial surface proteins. Can. J. Microbiol. 35, 215–227.

    Article  PubMed  CAS  Google Scholar 

  • Beveridge, T.J. (1981) Ultrastructure, chemistry, and function of the bacterial wall. Int. Rev. Cytol. 72, 229–317.

    Article  PubMed  CAS  Google Scholar 

  • Beveridge, T.J. and Graham, L.L. (1991) Surface layers of bacteria. Microbiol. Rev. 55, 684–705.

    PubMed  CAS  Google Scholar 

  • Beveridge, T.J. and Murray, R.G.E. (1975) Surface arrays on the cell wall of Spirillum metamorphum. J. Bacteriol. 124, 1529–1544.

    PubMed  CAS  Google Scholar 

  • Buckmire, F.L.A. and Murray, R.G.E. (1976) Substructure and in vitro assembly of the outer, structured layer of Spirillum serpens. J. Bacteriol. 125, 290–299.

    PubMed  CAS  Google Scholar 

  • Dubreuil, J.D., Logan, S.M., Cubbage, S., Ni Eidhin, D., McCubbin, W.D., Kay, C.M., Beveridge, T.J., Ferris, F.G. and Trust, T.J. (1988) Structural and biochemical analyses of a surface array protein of Campylobacter fetus. J. Bacteriol. 170, 4165–4173.

    PubMed  CAS  Google Scholar 

  • Kist, M.L. and Murray, R.G.E. (1984) Components of the regular surface array of Aquaspirillum serpens MW5 and their assembly in vitro. J. Bacteriol. 157, 599–606.

    PubMed  CAS  Google Scholar 

  • König, H. (1988) Archaeobacteria, in “Biotechnology” vol. 6b (Rehm, H.-J., Ed.), pp. 697–728. VCH Publishers, Weinheim.

    Google Scholar 

  • König, H. and Stetter, K.O. (1986) Studies on archaebacterial S-layers. System. Appl. Microbiol. 7, 300–309.

    Article  Google Scholar 

  • Koval, S.F. (1988) Paracrystalline protein surface arrays on bacteria. Can. J. Microbiol. 34, 407–414.

    Article  CAS  Google Scholar 

  • Koval, S.F. and Hynes, S.H. (1991) Effect of paracrystalline protein surface layers on predation by Bdellovibrio bacteriovorus. J. Bacteriol. 173, 2244–2249.

    PubMed  CAS  Google Scholar 

  • Koval, S.F. and Murray, R.G.E. (1984) The isolation of surface array proteins from bacteria. Can. J. Biochem. Cell Biol. 62, 1181–1189.

    Article  PubMed  CAS  Google Scholar 

  • Küpcü, S., Sara, M. and Sleytr, U.B. (1991) Chemical modification of crystalline ultrafiltration membranes and immobilization of macromolecules. J. Membrane Sci. 61, 167–175.

    Article  Google Scholar 

  • Lechner, J. and Wieland, F. (1989) Structure and biosynthesis of prokaryotic glycoproteins. Annu. Rev. Biochem. 58, 173–194.

    Article  PubMed  CAS  Google Scholar 

  • Lortal, S., van Heijenoort, J., Gruber, K. and Sleytr, U.B. (1992) S-layer of Lactobacillus helveticus ATCC 12046: isolation, chemical characterization and re-formation after extraction with lithium chloride. J. Gen. Microbiol. 138, 611–618.

    Article  CAS  Google Scholar 

  • Malcolm, A.J., Messner, P., Sleytr, U.B., Smith, R.H. and Unger, F.M. (1992) Crystalline bacterial cell surface layers (S-layers) as combined carrier/adjuvants for conjugate vaccines, in “Immobilised Macromolecules. Application Potentials in Biotechnology and Biosensors” (Sleytr, U.B., Messner, P., Pum, D. and Sara, M., Eds.), Springer-Verlag, London, in press.

    Google Scholar 

  • Marshall, K.C. (1991) The importance of studying microbial cell surfaces, in “Microbial Cell Surface Analysis. Structural and Physicochemical Methods” (Mozes, N., Handley, P.S., Busscher, H.J. and Rouxhet, P.G., Eds.), pp. 3–19. VCH Publishers, New York.

    Google Scholar 

  • Masuda, K. and Kawata, T. (1985) Reassembly of a regularly arranged protein in the cell wall of Lactobacillus buchneri and its reattachment to cell walls: chemical modification studies. Microbiol. Immunol. 29, 927–938.

    PubMed  CAS  Google Scholar 

  • Matteuzzi, D., Hollaus, F. and Biavati, B. (1978) Proposal of neotype for Clostridium thermohydrosulfiiricum and the merging of Clostridium tartarivorum with Clostridium thermosaccharolyticum. Int. J. Syst. Bacteriol. 28, 528–531.

    Article  Google Scholar 

  • Messner, P. and Sleytr, U.B. (1988 a)Asparaginyl-rhamnose: a novel type of protein-carbohydrate linkage in a eubacterial surface-layer glycoprotein. FEBS Lett. 228, 317–320.

    Article  PubMed  CAS  Google Scholar 

  • Messner, P. and Sleytr, U.B. (1988 b) Separation and purification of S-layers from gram-positive and gram-negative bacteria, in “Bacterial Cell Surface Techniques” (Hancock, I.C. and Poxton, I.R., Eds.), pp. 97–104. John Wiley & Sons, Chichester.

    Google Scholar 

  • Messner, P. and Sleytr, U.B. (1991) Bacterial surface layer glycoproteins. Glycobiology 1, 545–551.

    Article  PubMed  CAS  Google Scholar 

  • Messner, P. and Sleytr, U.B. (1992) Crystalline bacterial cell-surface layers, in “Advances in Microbial Physiology” vol. 33 (Rose, A.H. and Tempest, D.W., Eds.), pp. 213–275. Academic Press, London.

    Google Scholar 

  • Messner, P., Hollaus, F. and Sleytr, U.B. (1984) Paracrystalline cell wall surface layers of different Bacillus stearothermophilus strains. Int. J. Syst. Bacteriol. 34, 202–210.

    Article  Google Scholar 

  • Messner, P., Pum, D. and Sleytr, U.B. (1986) Characterization of the ultrastructure and the self-assembly of the surface layer of Bacillus stearothermophilus strain NRS 2004/3a. J. Ultrastruct. Mol. Struct. Res. 97, 73–88.

    Article  PubMed  CAS  Google Scholar 

  • Messner, P., Küpcü, S., Sára, M., Pum, D. and Sleytr, U.B. (1991) Characterization and biotechnological application of eubacterial glycoproteins, in “Protein Glycosylation: Cellular, Biotechnological and Analytical Aspects” (Conradt, H.S., Ed.), pp. 111–116. GBF Monographs, vol. 15. VHC-Verlagsgesellschaft, Weinheim.

    Google Scholar 

  • Messner, P., Christian, R., Kolbe, J., Schulz. G. and Sleytr, U.B. (1992 a) Analysis of a novel linkage unit of O-linked carbohydrates from crystalline surface layer glycoprotein of Clostridium thermohydrosulfiiricum S102-70. J. Bacteriol. 174, 2236–2240.

    PubMed  CAS  Google Scholar 

  • Messner, P., Mazid, M.A., Unger, F.M. and Sleytr, U.B. (1992 b) Artificial antigens. Synthetic carbohydrate haptens immobilized on crystalline bacterial surface layer glycoproteins. Carbohydr. Res., in press.

    Google Scholar 

  • Peters, J., Peters, M., Lottspeich, F. and Baumeister, W. (1989) S-layer protein gene of Acetogenium kivui: cloning and expression in Escherichia coli and determination of the nucleotide sequence. J. Bacteriol. 171, 6307–6315.

    PubMed  CAS  Google Scholar 

  • Pum, D., Sára, M. and Sleytr, U.B. (1989 a) Structure, surface charge, and self-assembly of the S-layer lattice from Bacillus coagulatis E38-66. J. Bacteriol. 171, 5296–5303.

    PubMed  CAS  Google Scholar 

  • Pum, D., Sára, M. and Sleytr, U.B. (1989 b) Use of two-dimensional protein crystals from bacteria for nonbiological applications. J. Vac. Sci. Technol. B 7, 1391–1397.

    Google Scholar 

  • Pum, D., Sára, M. and Sleytr, U.B. (1992 a) Two-dimensional (glyco)protein crystals as patterning elements and immobilisation matrices for the development of biosensors, in “Immobilised Macromolecules. Application Potentials in Biotechnology and Biosensors” (Sleytr, U.B., Messner, P., Pum, D. and Sára, M., Eds.), Springer-Verlag, London, in press.

    Google Scholar 

  • Pum, D., Sara, M., Messner, P. and Sleytr, U.B. (1992 b) Two-dimensional (glyco)protein crystals as patterning elements for the controlled immobilisation of functional molecules. Nanotechnology 4, 196–202.

    Google Scholar 

  • Sára, M. and Sleytr, U.B. (1987 a) Charge distribution on the S-layer of Bacillus stearothermophilus NRS 1536/3c and importance of charged groups for morphogenesis and function. J. Bacteriol. 169, 2804–2809.

    PubMed  Google Scholar 

  • Sára, M. and Sleytr, U.B. (1987 b) Molecular sieving through S-layers of Bacillus stearothermophilus strains. J. Bacteriol. 169, 4092–4098.

    PubMed  Google Scholar 

  • Sára, M. and Sleytr, U.B. (1987 c) Production and characteristics of ultrafiltration membranes with uniform pores from two-dimensional arrays of proteins. J. Membrane Sci. 33, 27–49.

    Article  Google Scholar 

  • Sára, M. and Sleytr, U.B. (1988) Membrane biotechnology: Two-dimensional protein crystals for ultrafiltration purposes, in “Biotechnology” vol. 6b (Rehm, H.-J., Ed.), pp. 615–636. VCH Publishers, Weinheim.

    Google Scholar 

  • Sára, M. and Sleytr, U.B. (1989) Use of regularly structured bacterial cell envelope layers as matrix for the immobilization of macromolecules. Appl. Microbiol. Biotechnol. 30, 184–189.

    Article  Google Scholar 

  • Sára, M., Küpcü, S. and Sleytr, U.B. (1989) Localization of the carbohydrate residue of the S-layerglycoprotein from Clostridium thermohydrosulfuricum LI 11-69. Arch. Microbiol. 151, 416–420.

    Article  Google Scholar 

  • Sára, M., Moser-Thier, K., Kainz, U. and Sleytr, U.B. (1990) Characterization of S-layers from mesophilic bacillaceae and studies on their protective role towards muramidases. Arch. Microbiol. 153, 209–214.

    Article  Google Scholar 

  • Sára, M., Pum, D. and Sleytr, U.B. (1992 a) Permeability and charge dependent adsorption properties of the S-layer lattice from Bacillus coagulans E38-66. J. Bacteriol. 174, 3487–3493.

    PubMed  Google Scholar 

  • Sára, M., Küpcü, S., Weiner, C, Weigert, S. and Sleytr, U.B. (1992 b) Crystalline protein layers as isoporous molecular sieves and immobilisation and affinity matrices, in “Immobilised Macromolecules. Application Potentials in Biotechnology and Biosensors” (Sleytr, U.B., Messner, P., Pum, D. and Sára, M., Eds.), Springer-Verlag, London, in press.

    Google Scholar 

  • Sjögren, A., Hovmöller, S., Farrants, G., Ranta, H., Haapsalo, M., Ranta, K. and Lounatmaa, K. (1985) Structures of two different surface layers found in six Bacteroides strains. J. Bacteriol. 164, 1278–1282.

    PubMed  Google Scholar 

  • Sleytr, U.B. (1975) Heterologous reattachment of regular arrays of glycoproteins on bacterial surfaces. Nature 257, 400–402.

    Article  PubMed  CAS  Google Scholar 

  • Sleytr, U.B. (1978) Regular arrays of macromolecules on bacterial cell walls: structure, chemistry, assembly, and function. Int. Rev. Cytol. 53, 1–64.

    Article  PubMed  CAS  Google Scholar 

  • Sleytr, U.B. and Messner, P. (1983) Crystalline surface layers on bacteria. Annu. Rev. Microbiol. 37, 311–339.

    Article  PubMed  CAS  Google Scholar 

  • Sleytr, U.B. and Messner, P. (1988 a) Crystalline surface layers on bacteria, in “Crystalline Bacterial Cell Surface Layers” (Sleytr, U.B., Messner, P., Pum, D. and Sára, M., Eds.), pp. 160–186. Springer-Verlag, Berlin.

    Chapter  Google Scholar 

  • Sleytr, U.B. and Messner, P. (1988 b) Crystalline surface layers in procaryotes. J. Bacteriol. 170, 2891–2897.

    PubMed  CAS  Google Scholar 

  • Sleytr, U.B. and Messner, P. (1989) Self-assemblies of crystalline bacterial cell surface layers, in “Electron Microscopy of Subcellular Dynamics” (Plattner, H., Ed.), pp. 13–31. CRC Press, Boca Raton.

    Google Scholar 

  • Sleytr, U.B. and Plohberger, R. (1980) The dynamic process of assembly of two-dimensional arrays of macromolecules on bacterial cell walls, in “Electron Microscopy at Molecular Dimensions” (Baumeister, W. and Vogell, W., Eds.), pp. 36–47. Springer-Verlag, Berlin.

    Chapter  Google Scholar 

  • Sleytr, U.B., Messner, P. and Pum, D. (1988) Analysis of crystalline bacterial surface layers by freeze-etching, metal shadowing, negative staining and ultrathin sectioning, in “Methods in Microbiology” vol. 20 (Mayer, F., Ed.), pp. 29–60. Academic Press, London.

    Google Scholar 

  • Sleytr, U.B., Sára, M. and Pum, D. (1989) Application potential of two dimensional protein crystals. Microelectronic Engineering 9, 13–20.

    Article  CAS  Google Scholar 

  • Sleytr, U.B., Pum, D., Sára, M. and Messner, P. (1992) Two-dimensional protein crystals as patterning elements in molecular nanotechnology, in “Proc. 2nd Int. Conference on Molecular Electronics-Science and Technology” (Aviram, A., Ed.). The Engineering Foundation, New York, in press.

    Google Scholar 

  • Smit, J. (1987) Protein surface layers of bacteria, in “Bacterial Outer Membranes as Model Systems” (Inouye, M., Ed.), pp. 343–376. John Wiley & Sons, New York.

    Google Scholar 

  • Smit, J. and Agabian, N. (1982) Cell surface patterning and morphogenesis: biogenesis of a periodic surface array during Caulobacter development. J. Cell Biol. 95, 41–49.

    Article  PubMed  CAS  Google Scholar 

  • Smith, S.H. and Murray, R.G.E. (1990) The structure and associations of the double S-layer on the cell wall of Aquaspirillum sinuosum. Can. J. Microbiol. 36, 327–335.

    Article  PubMed  CAS  Google Scholar 

  • Takeoka, A., Takumi, K., Koga, T. and Kawata, T. (1991) Purification and characterization of S layer proteins from Clostridium difficile GAI 0714. J. Gen. Microbiol. 137, 261–267.

    Article  PubMed  CAS  Google Scholar 

  • Thome, K.J.I., Oliver, R.C. and Glauert, A.M. (1976) Synthesis and turnover of the regularly arranged surface protein of Acinetobacter sp. relative to the other components of the cell envelope. J. Bacteriol. 127, 440–450.

    Google Scholar 

  • Tsuboi, A., Tsukagoshi, N. and Udaka, S. (1982) Reassembly in vitro of hexagonal surface arrays in a protein-producing bacterium, Bacillus brevis 47. J. Bacteriol. 151, 1485–1497.

    PubMed  CAS  Google Scholar 

  • Wicken, A.J. (1985) Bacterial cell walls and surfaces, in “Bacterial Adhesion. Mechanisms and Physiological Significance” (Savage, D.C. and Fletcher, M., Eds.), pp. 45–70. Plenum Press, New York.

    Chapter  Google Scholar 

  • Wildhaber, I., Santarius, U. and Baumeister, W. (1987) Three-dimensional structure of the surface protein of Desulfiirococcus mobilis. J. Bacteriol. 169, 5563–5568.

    PubMed  CAS  Google Scholar 

  • Yamada, H., Tsukagoshi, N. and Udaka, S. (1981) Morphological alterations of cell wall concomitant with protein release in a protein-producing bacterium, Bacillus brevis 47. J. Bacteriol. 148, 322–332.

    PubMed  CAS  Google Scholar 

  • Yang, L., Pei, Z., Fujimoto, S. and Blaser, M.J. (1992) Reattachment of surface array proteins to Campylobacter fetus cells. J. Bacteriol. 174, 1258–1267.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Zentrum für Ultrastrukturforschung und Ludwig-Boltzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180, Wien, Austria

    Paul Messner, Margit Sára, Dietmar Pum & Uwe B. Sleytr

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  1. Paul Messner
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  2. Margit Sára
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  3. Dietmar Pum
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  4. Uwe B. Sleytr
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Editors and Affiliations

  1. Centro de Biología Molecular, CSIC-UAM, Madrid, Spain

    M. A. de Pedro

  2. Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany

    J.-V. Höltje

  3. Institut für Allgemeine Biochemie, Universität Wien, Wien, Austria

    W. Löffelhardt

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Messner, P., Sára, M., Pum, D., Sleytr, U.B. (1993). Crystalline Bacterial Cell Surface Layers and their Application Potentials. In: de Pedro, M.A., Höltje, JV., Löffelhardt, W. (eds) Bacterial Growth and Lysis. Federation of European Microbiological Societies Symposium Series, vol 65. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9359-8_13

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  • DOI: https://doi.org/10.1007/978-1-4757-9359-8_13

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