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Bacterial Conjugation pp 53–73Cite as

Key Regulatory Aspects of Transfer of F-Related Plasmids

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Abstract

Current experiments designed to increase our understanding of the biochemistry of conjugation, as well as earlier experiments that mapped the transfer genes of the F sex factor, owe much of their design to the fact that transfer of the F sex factor is unregulated. Unregulated expression of transfer occurs because one of the critical regulatory genes of the transfer operon, the finO gene, was inactivated by the insertion of an IS3 sequence some time before F became a subject of study (6, 60). The existence of the moderately complex regulatory system in F transfer, to which this finO gene belongs, was first discovered by analysis of transfer of antibiotic resistance factors related to F (12, 54). In a series of elegant and insightful studies initially in the laboratory of Meynell (36, 37) and then in the laboratories of Anderson (la, 23, 46) and of Willetts (17–19), the basic control system for both F and related R factors such as R100 (also known as R222, NR1) was established several years ago. The final model proposed by Willetts (55) has stood against all challenges and is now generally accepted. A biological consequence of the “FinO-P” control system, as the model is now frequently called, is the subject of this chapter.

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References

  1. Abo, T., Inamoto S., and Ohtsubo, E., 1991, Specific DNA binding of the TraM protein to the onT region of plasmid R100, J. Bacteriol. 173: 6347–6354.

    PubMed  CAS  Google Scholar 

  2. la. Anderson, E. S., and Smith, H. R., 1972, Fertility inhibition in strains of Salmonella typhimurium, Mol. Gen. Genet. 118: 79–84.

    PubMed  CAS  Google Scholar 

  3. d’Aubenton-Carafa, Y., Brody, E., and Thermes, C., 1990, Prediction of rho-independent Escherichia coli transcription terminators. A statistical analysis of their RNA stem-loop structures, J. Mol. Biol. 216: 835–858.

    Article  PubMed  Google Scholar 

  4. Cheah, K-C., Hirst, R., and Skurray, R., 1987, finO sequences on conjugally repressed and derepressed F-like plasmids, Plasmid 17: 233–239.

    Google Scholar 

  5. Cheah, K C., Ray, A., and Skurray, R., 1984, Cloning and molecular analysis of the finO region from the antibiotic resistance plasmid R6–5, Plasmid 12: 222–226.

    Article  PubMed  CAS  Google Scholar 

  6. Cheah, K C., Ray, A., and Skurray, R., 1986, Expression of F plasmid traT: independence of traY-Z promoter and traf control, Plasmid 16: 101–107.

    Article  PubMed  CAS  Google Scholar 

  7. Cheah, K-C., and Skurray, R., 1986, The F plasmid carries an IS3 insertion within finO, J. Gen Microbiol. 132: 3269–3275.

    PubMed  CAS  Google Scholar 

  8. Dempsey, W. B., 1987, Transcript analysis of the plasmid R100 tral and finP genes, Mol. Gen. Genet. 209: 533–544.

    Article  PubMed  CAS  Google Scholar 

  9. Dempsey, W. B., 1989, Sense and antisense transcripts of traM, a conjugal transfer gene of the antibiotic resistance plasmid R100, Mol. Microbiol. 3: 561–570.

    Article  PubMed  CAS  Google Scholar 

  10. Dempsey, W. B., and Fee, B. E., 1990, Integration host factor affects expression of two genes at the conjugal transfer origin of plasmid R100, Mol. Microbiol. 4: 1019–1028.

    Article  PubMed  CAS  Google Scholar 

  11. de Smit, M. H., and van Duin, J., 1990, Control of prokaryotic translational initiation by mRNA secondary structure, in: Progress in Nucleic Acid Research and Molecular Biology, Vol. 38 ( J. Davidson), Academic Press, New York, pp. 1–35.

    Google Scholar 

  12. Di Laurenzio, L., Frost, L. S., Finlay, B. B., and Paranchych, W, 1991, Characterization of the onT region of the IncFV plasmid pED208, Mol. Microbiol. 5: 1779–1790.

    Article  PubMed  Google Scholar 

  13. lla. Di Laurenzio, L., Frost, L. S., and Paranchych, W, 1992, The TraM protein of the conjugative plasmid F binds to the origin of transfer of the F and ColE1 plasmids, Mol. Microbiol. 6: 2951–2959.

    Google Scholar 

  14. Egawa, R., and Hirota, Y., 1962, Inhibition of fertility by multiple drug resistance factor (R) in Escherichia coli K-12, Jap. J Genet. 37: 66–69.

    Article  Google Scholar 

  15. Fee, B. E., and Dempsey, W. B., 1986, Cloning, mapping, and sequencing of plasmid R100 traM and finP genes. J. Bacteriol. 167: 336–345.

    PubMed  CAS  Google Scholar 

  16. Fee, B. E., and Dempsey, W. B., 1988, Nucleotide sequence of gene X of antibiotic resistance plasmid RI00, Nucl. Acids Res. 16: 4726.

    Article  PubMed  CAS  Google Scholar 

  17. Figueroa, N., Wills, N., and Bossi, L., 1991, Common sequence determinants of the response of a prokaryotic promoter to DNA bending and supercoiling, EMBO J. 10: 941–949.

    PubMed  CAS  Google Scholar 

  18. Finlay, B. B., Frost, L. S., Paranchych, W, and Willetts, N. S., 1986, Nucleotide sequences of five IncF plasmid finP alleles, J. Bacteriol. 167: 754–757.

    PubMed  CAS  Google Scholar 

  19. Finnegan, D., and Willetts, N., 1972, The nature of the transfer inhibitor of several F-like plasmids, Mol. Gen. Genet. 119: 57–66.

    Article  PubMed  CAS  Google Scholar 

  20. Finnegan, D., and Willetts, N., 1973, The site of action of the F transfer inhibitor, Mol. Gen. Genet. 127: 307–316.

    Article  PubMed  CAS  Google Scholar 

  21. Finnegan, D. J., and Willetts, N. S., 1971, Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor, Mol. Gen. Genet. 111: 256–264.

    Article  PubMed  CAS  Google Scholar 

  22. Friedman, D. I., 1988, Integration host factor: a protein for all reasons, Cell 55: 545–554.

    Article  PubMed  CAS  Google Scholar 

  23. Frost, L., Lee, S., Yanchar, N., and Paranchych, W., 1989, finP and fs0 mutations in finP anti-sense RNA suggest a model for FinOP action in the repression of bacterial conjugation by the Flac plasmid JCFLO, Mol. Gen. Genet. 218: 152–160.

    Google Scholar 

  24. Goodrich, J. A., Schwartz, M. L., and McClure, W. R., 1990, Searching for and predicting the activity of sites for DNA binding proteins. compilation and analysis of the binding sites for Escherichia coli integration host factor (IHF), Nucl. Acids Res. 18: 4993–5000.

    Article  PubMed  CAS  Google Scholar 

  25. Grindley, N. D., Grindley, J. N., Smith, H. R., and Anderson, E. S., 1973, Characterisation of derepressed mutants of an F-like R factor, Mol. Gen. Genet. 120: 27–34.

    Article  PubMed  CAS  Google Scholar 

  26. Inamoto, S., and Ohtsubo, E., 1990, Specific binding of the TraY protein to oriT and the promoter region for the traY gene of plasmid R100, J. Biol. Chem. 265: 6461–6466.

    PubMed  CAS  Google Scholar 

  27. Inamoto, S., Yoshioka, Y., and Ohtsubo, E., 1988, Identification and characterization of the products from the traJ and traY genes of plasmid R100, J. Bacteriol. 170: 2749–2757.

    PubMed  CAS  Google Scholar 

  28. Inamoto, S., Abo, T., and Ohtsubo, E., 1990, Binding sites of integration host factor in or:T of plasmid R100, J. Gen. Appl. Microbiol. 36: 287–293.

    Article  CAS  Google Scholar 

  29. Inamoto, S., Yoshioko, Y., and Ohtsubo, E., 1991, Site-and strand-specific nicking in vitro at onT by the TraY-Trac endonuclease of plasmid R100, J. Biol. Chem. 266: 10086–10092.

    PubMed  CAS  Google Scholar 

  30. Jaeger, J. A., Turner, D. H., and Zuker, M., 1989, Improved predictions of secondary structures for RNA, Proc. Natl. Acad. Sci. USA 86: 7706–7710.

    Article  PubMed  CAS  Google Scholar 

  31. Kittle, J. D., Simons, R. W, Lee, J., and Kleckner, N., 1989, Insertion sequence IS10 anti-sense pairing initiates by an interaction between the 5’ end of the target RNA and a loop in the anti-sense RNA, J. Mol. Biol. 210: 561–572.

    Article  PubMed  CAS  Google Scholar 

  32. Koraimann, G., Koraimann, C., Koronakis, V., Schlager, S., and Högenauer, G., 1991, Repression and derepression of conjugation of plasmid RI by wild-type and mutated fnP antisense RNA, Mol. Microbiol. 5: 77–87.

    Article  PubMed  CAS  Google Scholar 

  33. Koronakis, V. E., Bauer, E., and Högenauer, G., 1985, The traM gene of the resistance plasmid R1: comparison with the corresponding sequence of the Escherichia coli F factor, Gene 36: 79–86.

    Article  PubMed  CAS  Google Scholar 

  34. Lahue, E. E., and Matson, S. W., 1990, Purified Escherichia coli F-factor TraY protein binds onT, J. Bacteriol. 172: 1385–1391.

    PubMed  CAS  Google Scholar 

  35. Masukata, H., and Tomizawa, J., 1990, A mechanism of formation of a persistent hybrid between elongating RNA and template DNA, Cell 62: 331–338.

    Article  PubMed  CAS  Google Scholar 

  36. Matson, S. W, 1991, DNA helicases of Escherichia coli, in: Progress in Nucleic Acid Research and Molecular Biology, Vol. 40 ( J. Davidson), Academic Press, New York, pp. 289–326.

    Google Scholar 

  37. Matson, S. W, and Kaiser-Rogers, K. A., 1990, DNA helicases, Ann. Rev. Biochem. 59: 289–329.

    Article  PubMed  CAS  Google Scholar 

  38. Matson, S. W, and Morton, B. W, 1991, Escherichia coli DNA helicase I catalyzes a site-and strand-specific nicking reaction at the F plasmid oriT, J. Biol. Chem. 266: 16232–16237.

    CAS  Google Scholar 

  39. McIntire, S. A., and Dempsey, W. B., 1987, Fertility inhibition gene of plasmid R100, Nucl. Acids Res. 15: 2029–2042.

    Article  PubMed  CAS  Google Scholar 

  40. Meynell, E., and Cooke, M., 1969, Repressor-minus and operator constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC, Genet. Res. 14: 309–313.

    Article  PubMed  CAS  Google Scholar 

  41. Meynell, E., and Datta, N., 1967, Mutant drug resistance factors of high transmissibility, Nature 214: 885–887.

    Article  PubMed  CAS  Google Scholar 

  42. Nakaya, R., Nakamura, A., and Murata, Y., 1960, Resistance transfer agents in Shigella, Biochem. Biophys. Res. Commun. 3: 654–659.

    Article  CAS  Google Scholar 

  43. Persson, C., Wagner, E. G. H., and Nordstrom, K., 1990, Control of replication of plasmid R1: formation of an initial transient complex is rate limiting for antisense RNA-target RNA pairing, EMBO J. 9: 3777–3785.

    PubMed  CAS  Google Scholar 

  44. Polisky, B., Mang, X. Y., and Fitzwater, T., 1990, Mutations affecting primer RNA interaction with the replication repressor RNA I in plasmid CoIEI: potential RNA folding pathway mutants, EMBO J. 9: 295–304.

    PubMed  CAS  Google Scholar 

  45. Reygers, U., Wessel, R., Müller, H., and Hoffmann-Berling, H., 1991, Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor, EMBO J. 10: 2689–2694.

    PubMed  CAS  Google Scholar 

  46. Richardson, J. P., 1990, Rho-dependent transcription termination, Biochim. Biophys. Acta. 1048: 127–138.

    Article  PubMed  CAS  Google Scholar 

  47. Schwab, M., Gruber, H., and Högenauer, G., 1991, The TraM protein of plasmid RI is a DNA-binding protein, Mol. Microbiol. 5: 439–446.

    Article  PubMed  CAS  Google Scholar 

  48. Silverman, P. M., Wickersham, E., and Harris, R., 1991, Regulation of the F plasmid traY promoter in Escherichia coli by host and plasmid factors, J. Mol. Biol. 218: 119–128.

    Article  PubMed  CAS  Google Scholar 

  49. Silverman, P M., Wickersham, E., Rainwater, S., and Harris, R., 1991, Regulation of the F plasmid traY promoter in Escherichia coli K12 as a function of sequence context, J. Mol. Biol. 220: 271–279.

    Article  PubMed  CAS  Google Scholar 

  50. Smith, H. R., Humphreys, G. O., Grindley, N. D., Grindley, J. N., and Anderson, E. S, 1973, Molecular studies of an ft+ plasmid from strains of Salmonella typhimurium, Mol. Gen. Genet. 126: 143–151.

    Article  PubMed  CAS  Google Scholar 

  51. Thompson, R., and Taylor, L., 1982, Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ, Mol. Gen. Genet. 188: 513–518.

    Article  PubMed  CAS  Google Scholar 

  52. Mullis, K. N., Andres, I., and Achtman, M., 1978, Fertility repression of F-like conjugative plasmids: physical mapping of the R6–5 frnO andfinP cistrons and identification of the Fin() protein, Proc. Natl. Acad. Sci. USA 75: 5836–5840.

    Article  Google Scholar 

  53. Tomizawa, J., 1986, Control of ColE1 plasmid replication: binding of RNA Ito RNA II and inhibition of primer formation, Cell 47: 89–97.

    Article  PubMed  CAS  Google Scholar 

  54. Tsai, M-M., Fu, F., and Deonier, R. C., 1990, Intrinsic bends and integration host factor binding at F plasmid ortT, J. Bacteriol. 172: 4603–4609.

    PubMed  CAS  Google Scholar 

  55. Thrner, D. H., Sugimoto, N., and Freier, S. M., 1988, RNA structure prediction, Ann. Rev. Biophys. Biophys. Chem. 17: 167–192.

    Article  Google Scholar 

  56. Titriser, D. H., Sugimoto, N., Jaeger, J. A., Longfellow, C. E., Freier, S. M., and Kierzek, R., 1987, Improved parameters for prediction of RNA structure, Cold Spring Harbor Symp. Quant. Biol. 52: 123–133.

    Article  Google Scholar 

  57. Watanabe, T., 1963, Infective heredity of multiple drug resistance in bacteria, Bacteriol. Rev. 27: 87–115.

    PubMed  CAS  Google Scholar 

  58. Watanabe, T., and Fukasawa, T., 1961, Episome-mediated transfer of drug resistance in Enterobacteriaceae. I. Transfer of resistance factors by conjugation, J. Bacteriol. 81: 669–678.

    PubMed  CAS  Google Scholar 

  59. Willetts, N., 1977, The transcriptional control of fertility of F-like plasmids, J. Mol. Biol. 112: 141–148.

    Article  PubMed  CAS  Google Scholar 

  60. Willetts, N., and Skurray, R., 1987, Structure and function of the F factor and mechanism of conjugation, in: Escherichia coli and Salmonella typhimurium: cell and molecular biology (F. C. Neidhardt), American Society for Microbiology, Washington, DC, pp. 1110–1133.

    Google Scholar 

  61. Womble, D. D., and Rownd, R. H., 1986, Regulation of IncF1I plasmid DNA replication, J. Mol. Biol. 192: 529–548.

    Article  PubMed  CAS  Google Scholar 

  62. Yang, C-C., and Nash, H. A., 1989, The interaction of E. coli IHF protein with its specific binding sites, Cell 57: 869–880.

    Article  PubMed  CAS  Google Scholar 

  63. Yoshioka, Y., Fujita, Y., and Ohtsubo, E., 1990, Nucleotide sequence of the promoter-distal region of the tra operon of plasmid R100, including tral (DNA Helicase I) and traD genes, J. Mol. Biol. 214: 39–53.

    Article  PubMed  CAS  Google Scholar 

  64. Yoshioka, Y., Ohtsubo, H., and Ohtsubo, E., 1987, Repressor gene fn0 in plasmids R100 and F: constitutive transfer of plasmid F is caused by insertion of IS3 into F finO, J. Bacteriol. 169: 619–623.

    PubMed  CAS  Google Scholar 

  65. Zuker, M., 1989, Computer prediction of RNA structure, in: Methods in Enzymology, Vol. 180A ( J. E. Dahlberg and J. N. Abelson), Academic Press, New York, pp. 262–288.

    Google Scholar 

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  1. University of Texas Health Science Center and Department of Veterans Affairs, Veterans Affairs Medical Center, Dallas, Texas, 75216, USA

    Walter B. Dempsey

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  1. Department of Biologic and Materials Sciences, School of Dentistry and Department of Microbiology and Immunology, School of Medicine, The University of Michigan, 48109-0402, Ann Arbor, Michigan, USA

    Don B. Clewell

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Dempsey, W.B. (1993). Key Regulatory Aspects of Transfer of F-Related Plasmids. In: Clewell, D.B. (eds) Bacterial Conjugation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9357-4_3

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  • DOI: https://doi.org/10.1007/978-1-4757-9357-4_3

  • Publisher Name: Springer, Boston, MA

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