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High Performance Capillary Electrophoresis of Proteins and Peptides: A Minireview

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Applications of Enzyme Biotechnology

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Abstract

The purpose of this minireview is to summarize recent advances in peptide and protein high performance capillary electrophoresis (HPCE) for the protein chemist. The review is intended to be general and assumes that the reader is planning to enter the field to solve protein related problems. Therefore, I will focus on applications directed to protein and peptide chemistry, and attempt to provide the requisite HPCE background. HPCE is an instrumental approach to electrophoresis, introduced by Mikkers et al. (1) and Jorgenson and Lukas (2), that is finding increasing application in the area of protein and peptide chemistry. As such, it offers the scientist several advantages: (A) sensitive online detection without the normal postrun manipulations of staining, destaining or blotting to detect the proteins; (B) rapid analysis times; (C) automation with computer controlled HPCE systems, thus, increased quantitation and sample throughput and (D) requires small sample volumes. Diverse separation methodologies are being developed that produce greatly increased resolution compared to routine protein electrophoresis methodologies.

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Authors and Affiliations

  1. Barnett Institute, Northeastern University, Boston, MA, 02115, USA

    Robert S. Rush

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  1. Texas A&M University, College Station, Texas, USA

    Jeffery W. Kelly  & Thomas O. Baldwin  & 

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Rush, R.S. (1991). High Performance Capillary Electrophoresis of Proteins and Peptides: A Minireview. In: Kelly, J.W., Baldwin, T.O. (eds) Applications of Enzyme Biotechnology. Industry-University Cooperative Chemistry Program Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9235-5_18

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  • DOI: https://doi.org/10.1007/978-1-4757-9235-5_18

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