Abstract
The chorioallantoic membrane (CAM) of the chick is formed by fusion of the somatic mesoderm of the chorion with the splachnic mesoderm of the allantois during the 4th to 5th day of embryonic development. After 6 days of incubation, the CAM is covering a surface area of approximately 6 cm2. By day 10, and extending to day 14, mean surface area of the CAM is approximately 65 cm2. This expansion of CAM is accompanied by an increased complexity of the patterns of capillary microvessels. The number of capillary vessels per square centimetre is increased about 150% from day 7 to day 12 and the intercapillary distances have been reduced about 40% during the same period (1). This highly vascularized membrane serves as the first respiratory system of the avian.
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References
Defouw, O.D., Rizzo, V.J., Steinfeld, R. and Feinberg, R.N. (1989). Mapping of the microcirculation in the chick chorioallantoic membrane during normal angiogenesis. Microvasc. Res., 38: 136–147.
D’Amore, P.A. and Thompson, R.N. (1987). Mechanism of angiogenesis. Ann. Rev. Physiol., 49: 453–464.
Maragoudakis, M.E., Panoutsakopoulou, M. and Sarmonica, M. (1988). Rate of basement membrane biosynthesis as an index of angiogenesis. Tissue and Cell, 20: 531–539.
Maragoudakis, M.E., Missirlis, E., Sarmonika, M., Panoutsakopoulou, M. and Karakioulakis, G. (1989). Basement membrane biosynthesis as a target to tumour therapy. J. Pharmacol. Exp. Ther., 252: 753–757.
Karakioulakis, G., Missirlis, E., Aletras, A. and Maragoudakis, M.E. (1988). Degradation of intact basement membranes by human and murine tumour enzymes. Biochim. Biophys. Acta. 967: 163–175.
Harris-Hooker, S.A., Gajdusek, C.M, Wight, T.N. and Schwartz, S.M. (1983). Neovascular responses induced by cultured aortic endothelial cells. J. Cell. Physiol., 114: 302–310.
Scroggs, A.W., Proia, A.D., Smith, C.F., Halperin, E.C. and Klintworth, G.K. (1991). The effect of total body irradiation on cornea neovascularization in the fi scher 344 rat after chemical cauterisation. Invest. Ophthalmol. Visual. Sci., 32: 2105–2111.
Kowalski, J., Khwan, H.H., Prionas, S.D., Allison, A.C. and Fajardo, L.F. (1992). Characterisation and application of the disc angiogenesis system. Exp. Mol., 56: 1–19.
Strick, D.M., Waycarter, R.L., Montani, J.P., Gay, W.J. and Adair, T.H. (1991). Morphometric measurements on chorioallantoic membrane vascularity: Effects of hypoxia and hyperoxia. Am. J. Physiol., 260:H1385–H1389.
Maragoudakis, M.E., Haralabopoulos, G.C., Tsopanoglou, N.E. and Pipili-Synetos, E. (1995). Validation of collagenous protein synthesis as an index for angiogenesis with the use of morphological methods. Microvasc. Res., 50:215–222.
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Tsopanoglou, N.E., Maragoudakis, M.E. (1998). Basement Membrane Biosynthesis as a Biochemical Index of Angiogenesis in Chick Chorioallantoic Membrane. In: Maragoudakis, M.E. (eds) Angiogenesis. NATO ASI Series, vol 298. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9185-3_4
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DOI: https://doi.org/10.1007/978-1-4757-9185-3_4
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