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Studies on the Mechanism of Estradiol Uptake by Rat Uterine Cells and on Estradiol Binding to Uterine Plasma Membranes

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Steroid Hormone Receptor Systems

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 117))

Summary

A method for the preparation of viable uterine cell suspensions is described. Using this system the kinetics of estradiol uptake were studied in order to assess whether the steroid enters uterine cells by passive diffusion (1) or protein mediated diffusion (2). The data presented show that a) the rates of estradiol entry are nonsaturable; b) temperature dependence of uptake kinetics gives a linear Arrhenius plot; c) E2-6-CMO-BSA does not inhibit 3H-E2 uptake; d) uterine plasma membranes do not contain specific estrogen binding sites. Thus, estrogen uptake occurs by passive diffusion.

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Author information

Authors and Affiliations

  1. Biochemistry Department, Boston University School of Medicine, Boston, Massachusetts, USA

    R. E. Muller, T. C. Johnston, A. M. Traish & H. H. Wotiz

Authors
  1. R. E. Muller
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  2. T. C. Johnston
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  3. A. M. Traish
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  4. H. H. Wotiz
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Editor information

Editors and Affiliations

  1. Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts, USA

    Wendell W. Leavitt

  2. Baylor College of Medicine, Houston, Texas, USA

    James H. Clark

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© 1979 Springer Science+Business Media New York

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Muller, R.E., Johnston, T.C., Traish, A.M., Wotiz, H.H. (1979). Studies on the Mechanism of Estradiol Uptake by Rat Uterine Cells and on Estradiol Binding to Uterine Plasma Membranes. In: Leavitt, W.W., Clark, J.H. (eds) Steroid Hormone Receptor Systems. Advances in Experimental Medicine and Biology, vol 117. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6589-2_22

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  • DOI: https://doi.org/10.1007/978-1-4757-6589-2_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-6591-5

  • Online ISBN: 978-1-4757-6589-2

  • eBook Packages: Springer Book Archive

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