Abstract
The method presented in this article for the mechanical stimulation of cells via specific receptors was adapted from the method of Wang and Ingber.1,2 Unlike most current methods which apply strain to the cell as a whole, our method is able to determine the specific receptors involved in stress transduction. The stress is applied using ferromagnetic microbeads coated with receptor-specific ligands. After adhesion to cells, the beads are magnetized and then “twisted” using a magnetic field oriented perpendicular to the magnetizing field. This method was applied to osteoblastic cells, and it could be shown that mechanical stress on integrin receptors induces specific tyrosine phosphorylation.
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Bierbaum, S., Notbohm, H. (1997). Magnetomechanical Stimulation of Mesenchymal Cells. In: Häfeli, U., Schütt, W., Teller, J., Zborowski, M. (eds) Scientific and Clinical Applications of Magnetic Carriers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6482-6_23
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DOI: https://doi.org/10.1007/978-1-4757-6482-6_23
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