Abstract
The central dark line (CDL) which is one of the 100 planes of hydroxyapatite and represents the site of initiation of crystal growth, was observed not only in the crystallites of enamel but in dentin, bone and baleen as well. The area of CDL probably contains high concentration of carbonate ions. It was demonstrated in all the hard tissues tested that the earliest appearance of mineral structure is composed of a ribbon- or disc-shaped precursor mineral plate intimately surrounded by an organic envelope. The CDL appears at the center of the mineral portion as the first step in crystal growth.
Carbonic anhydrase activity was observed in the area where crystal nucleation occurs in developing enamel, dentin, cartilage and bone. The enzyme probably contributes the carbonate ion to the crystal nucleation site where the carbonate-rich CDL is being formed. By means of immunoblotting method it has been shown that carbonic anhydrase is one of the major protein constituents of first formed enamel.
The similarities in the mineralization process among the apatite forming tissues suggest that the matrices of these hard tissues contain similar groups of proteins, probably derived from basic cytoplasmic proteins. Some of these proteins such as troponin T and I, actin and myosin, were in fact identified in developing enamel matrix by means of the immunoblotting technique. The presence of troponins and actin were also demonstrated in calcifying dentin. Calmodulin was shown to be present only in the later (maturing) stage of enamel. These cytoplasmic proteins contained in the calcifying matrix are in a somewhat altered condition compared with their original form in the cytoplasm. The alteration is probably due to the results of the complicated process of degradation and recombination that occurs in these proteins.
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References
ARENDS, J.,1982. Mechanism of dental caries. In Biological Mineralization and Demineralization (ed. G.H. Nancollas), pp. 303–324. Berlin: Springer-Verlag.
BOCCIARELLI, D.S., 1970. Morphology of crystallites in bone. Cale. Tiss. Res., 5:261–269.
DEUTSCH, D., SHAPIRA, L., ALAYOFF, A., LEVIEL, D., YOELI, Z. & ARAD, A., 1984. Protein and mineral changes during prenatal and postnatal development and mineralization of human deciduous enamel. In Tooth Enamel IV (ed. R.W. Fearnhead & S. Suga). pp. 234–239, Amsterdam: Elsevier Science Publishers.
EASTOE, J.E., 1979. Enamel protein chemistry-past, present and future. J. Den. Res., 58 (B):753–763.
FINCHAM, A.G., 1984. Amelognins: Progress and problems. In Tooth Enamel IV (ed. R.W. Fearnhead & S. Suga), pp. 114–119, Amsterdam: Elsevier Science Publishers.
HALLSWORTH, A.S., WEATHERELL, J.A. & ROBINSON, C., 1973. Loss of carbonate during the first stage of enamel caries. Caries Res., 7:345–348.
HALSTEAD, L.B., 1974. Vertebrate Hard Tissues. pp. 100–102. London: Wykeham Publications.
HILLER, C.R, ROBINSON, C. & WEATHERALL, J.A., 1975. Variations in the composition of developing rat incisor enamel. Cale. Tiss. Res., 18:1–12.
JOHANSEN, E., 1963. Ultrastructural and chemical observations on dental caries. In Mechanisms of Hard Tissue Destruction (ed. R.F. Sognnaes), pp. 187–211, Washington, D.C., Amer. Assoc. Adv. Sci.
JOHANSEN, E. & PARKS, H.F., 1960. Electron microscopic observations on the three-dimensional morphology of apatite crystallites of human dentin and bone. J. Biophys. Biochem. Cytol., 7:743–746.
KAKEI, M. & NAKAHARA, H., 1983a. A light microscopic study of the localization of carbonic anhydrase activity in the developing dentin and enamel of the rat lower incisor. Jap. J. Oral Biol., 25:374–377.
KAKEI, M. & NAKAHARA, H. 1983b. Ultrastructural localization of carbonic anhydrase activity in developing enamel and dentin of the rat incisor. Jap. J. Oral Biol., 25:1129–1133.
KAKEI, M., & NAKAHARA, H., 1984. Histochemical localization of carbonic anhydrase activity in epiphyseal growth cartilage and calvaria of rat. Jap. J. Oral Biol., 26:554–558.
KAKEI, M., & NAKAHARA, H., 1985a. Electroimmunoblotting study of carbonic anhydrase in developing enamel and dentin of the rat lower incisor. Jap. J. Oral Biol., 27:357–361.
KAKEI, M., & NAKAHARA, H., 1985b. Demonstration of cytoplasmic structural proteins in developing enamel matrix of the rat incisor. Jap. J. Oral Biol., 27:1001–1005.
KAKEI, M. & NAKAHARA, H., 1986. Identification of calmodulin in developing enamel matrix by the immunoblotting technique. Bull. Josai Dent. Univ., 15:309–312.
LAEMMLI, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680–685.
LOWENSTAM, H.A. & MARGULIS, L., 1980a. Calcium regulation and the appearance of calcareous skeleton in the fossil record. In The Mechanisms of Biomineralization in Animals and Plants (ed. M. Omori & N. Watabe), pp. 289–300, Tokyo: Tokai University Press.
LOWENSTAM, II.A. & MARGULIS, L., 1980b. Evolutionary prerequisites for early phanerozoic calcareous skeletons. BioSystems, 12:27–41.
MARSHALL, A.F. & LAWLESS, K.R., 1981. TEM study of the central dark line in enamel crystallites. J. Den. Res., 60:1773–1782.
NAKAHARA, H., 1982. Electron microscopic studies of the lattice image & “central dark line” of crystallites in sound and carious human dentin. Bull. Josai Dent. Univ., 11:209–215.
NAKAHARA, H. & KAKEI, M., 1983. The central dark line in developing enamel crystallite: An electron microscopic study. Bull. Josai Dent. Univ., 12:1–7.
NAKAHARA, H., & KAKEI, M., 1984a. TEM observations on the crystallites of dentin and bone. Bull. Josai Dent. Univ., 13:259–263.
NAKAHARA, H. & KAKEI, M., 1984b. Central dark line and carbonic anhydrase: Problems relating to crystal nucleation in enamel. In Tooth Enamel IV. (ed. R.W. Fearnhead, & S. Suga) pp. 42–46, Amsterdam: Elsevier Science Publishers.
OFARRELL, P.H., 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 250:4007–4021.
PAUTARD, F.G.E., 1963. Mineralization of keratin and its comparison with the enamél matrix. Nature, 199:531–535.
QUINT, P., ALTHOFF, J., HÖHLING, H.J., BOYDE, A. & LAABS, W.A., 1980. Characteristic molar ratios of magnesium, carbon dioxide, calcium and phosphorus in the mineralizing fracture callus and predentin. Calc. Tiss. Intern., 32:257–261.
ROBINSON, C., BRIGGS, H.D., ATKINSON, P.J. & WEATHERELL, J.A., 1979. Matrix and mineral changes in developing enamel. J. Den. Res., 58(b):871–880.
TAKEYAMA, H. & KIYOMURA, H., 1984. A histochemical study of carbonic anhydrase activity in alveolar bone of the rat. J. Jap. Orthod. Soc., 43:356–360.
TAKUMA, S., 1971. Dentin caries. In A New Atlas of Dental Pathology, 2nd Edition (ed. S. Takuma), pp. 138–183. Tokyo: Ishiyaku Shuppan. (In Japanese).
TOWBIN, H., STAEIIELIN, T. & GOROLON, J., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Nat. Acad. Sci. U.S.A., 79:4350–4354.
VOGEL, J.C. & FRANK, R.M., 1977a. Stages in the dissolution of human enamel crystals in dental caries. Calc. Tiss. Res., 24:19–27.
VOGEL, J.C. & FRANK, R.M., 1977b. Ultrastructural study of apatite crystal dissolution in human dentin and bone. J. Biol. Buccale, 5:181–194.
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Nakahara, H., Kakei, M. (1989). Ultrastructural and Protein Aspects of Apatite Formation in Vertebrate Hard Tissues. In: Crick, R.E. (eds) Origin, Evolution, and Modern Aspects of Biomineralization in Plants and Animals. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6114-6_16
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DOI: https://doi.org/10.1007/978-1-4757-6114-6_16
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