Abstract
A characteristic of skeleton formation in the hermatypic coral Acropora hebes has been investigated with special reference to the calcium-binding substance assay. The skeletal soluble organic matrix such as protein and carbohydrate did not bind calcium ions in the Sephadex G-75 chromatography. On the other hand, the insoluble organic matrix of the skeleton showed the calcium-binding ability, which was reduced by treatment with the organic solvent. Presumably, the calcium-binding substances in the skeletal insoluble matrix are firmly attached to the calcium carbonate crystals and will not be extracted by the aqueous or organic extracting solutions. The major component of the calcium-binding substances in the skeleton is phospholipid, but not the other lipids such as neutral lipid or glycolipid. A high-performance TLC revealed that the skeletal calcium-binding phospholipids consisted mainly of acidic phosphatidylserine.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
ABANTANGELO, G., DAGA-GORDINI, D., CASTELLANI, I. and CORTIVO, R., 1978. Some observations on the calcium ion binding to the eggshell matrix. Calcif. Tiss. Res., 26: 247–252.
ABOLINS-KROGIS, A., 1979. The effect of adenosine triphosphate, magnesium chloride and phospholipids on crystal formation in the mineralized shell-repair membrane of the snail, Helix pomatia L. Cell Tissue Res., 204: 497–505.
BOSKEY, A.L. and POSNER, A.S., 1976. Extraction of a calcium-phospholipid-phosphate complex from bone. Calcif. Tissue Res., 19: 273–283.
BOSKEY, A.L., GOLDBERG, M.R. and POSNER, A.S., 1978. Calcium-phospholipid-phosphate complexes in mineralized tissue. Proc. Soc. Exp. Biol. Med., 157: 590–593.
COTMORE, J.M., NICHOLS, G. and WUTHEIR, R.E., 1971. Phospholipid-calcium migration in the presence of phosphate. Science, 172: 1339–1341.
CRENSHAW, M.A., 1972. The soluble matrix from Mercenaria mercenaria shell. Biomineralization, 6: 6–11.
DITTER, J.D. and LESTER, R.L., 1964. A simple specific spray for the determination of phospholipids on thin-layer chromatography. J. Lipid Res., 5: 126–127.
GOREAU, T.F., 1956. Histochemical detection of mucopolysaccharide-like substances and alkaline phosphatase in madreporaria. Nature, 177: 1020.
I-IODGE, J.E. and HOFREITER, B.T., 1962. Determination of reducing sugars and carbohydrates. In Methods in Carbohydrate Chemistry. pp. 388. Ed. by R.L. Whistler and M.L. Wolfrom. Academic Press.
HSU, H.H.T., CECIL, R.N.A. and ANDERSON, H.C., 1978. Role of adenosine triphosphatase, phospholipid and vesicular structure in the calcification of isolated and reconstituted matrix vesicles. Metabl Bone Dis. Rel. Res., 1: 169–172.
ISA, Y. and YAMAZATO, K., 1981. The ultrastructure of calicoblast and related tissues in Acropora hebes (Dana). Proc. 4th Int. Coral Reef Symp., 2: 99–105.
ISA, Y., 1986. An electron microscope study on the mineralization of the skeleton of the staghorn coral, Acropora hebes. Mar. Biol., 93: 91–101.
ISA, Y. and OKAZAKI, M., 1987. Some observations on the Ca binding phospholipid from scleractinian coral skeletons. Comp. Biochem. Physiol., 87b: 507–512.
ISA, Y. and IKEDA, K., 1986. Some properties of the Ca binding substance of spicule from the alcyonarian corals. Ryudai RI Lab. Reports., 2:1–4.
JOHNSTON, I.S., 1979. The organization of a structural organic matrix within the skeleton of a reef-building coral. Scann. Electron Microsc., 2: 421–431.
JOHNSTON, I.S., 1980. The ultrastructure of skeletogenesis in hermatypic corals. In International review of cytology, vol. 67, pp. 71–214. Ed. by H. Bourne and J.F. Danielle, New York Academic Press.
MTITERER, R.M., 1978. Amino acid composition and metal binding capacity of the skeletal protein of corals. Bull. Mar. Sci., 28: 173–180.
MAJESKA, R.J., HOLWERDA, D.L. and WUTHEIR, R.E., 1979. Localization of phosphatidylserine in isolated chick epiphyseal cartilage matrix vesicles with trinitrobenzene-sulfonate. Calcif. Tissue Intl., 141: 41–46.
RABINOWITZ, J.L., TRAVARES, C.J., LIPSON, R. and PERSON, P., 1976. Lipid components and in vitro mineralization of some invertebrate cartilages. Biol. Bull., 150: 69–79.
SAMATA, T. and KRAMPITz, G., 1982. Calcium-binding polypeptides in oyster shells. Malacologia, 22: 225–233.
WAINWRIGHT, S.A., 1963. Skeletal organization in the coral, Pocillopora damicornis. Quart. J. Micr. Sci., 104: 169–183.
WUTHEIR, R.E., 1975. Lipid composition of isolated epiphyseal cartilage cells, membranes and matrix vesicles. Biochim. Biophys. Acta, 409: 128–143.
YOUNG, S.D., 1971a. Organic material from scleractinian coral skeletons - I. Variation in composition between several species. Comp. Biochem. Physiol., 40B: 113–120.
YOUNG, S.D., O’CONNER, J.D. and MIUSCATINE, L., 1971. Organic material from scleractinian coral skeletons - II. Incorporation of C into protein, chitin and lipid. Comp. Biochem. Physiol., 40B: 945–958.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Isa, Y. (1989). Calcium Binding Substance in the Hermatypic Coral, Acropora Hebes (Dana). In: Crick, R.E. (eds) Origin, Evolution, and Modern Aspects of Biomineralization in Plants and Animals. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6114-6_12
Download citation
DOI: https://doi.org/10.1007/978-1-4757-6114-6_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-6116-0
Online ISBN: 978-1-4757-6114-6
eBook Packages: Springer Book Archive