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Phospholipid Exchange between Membranes

  • Chapter
Methods in Membrane Biology

Abstract

Exchange of phospholipids between serum lipoproteins was described more than 20 years ago by Eder et al. (1954) and by Kunkel and Beam (1954). A few years later, Florsheim and Morton (1957) showed that phospholipid exchange between serum lipoprotein fractions is rapid compared to the disappearance of these lipids from plasma. Exchange of phospholipids between chylomicrons and higher density serum lipoprotein was demonstrated by McCandless and Zilversmit (1958). These studies were extended by Minari and Zilversmit (1963), who showed that the loss of labeled phospholipids from chylomicrons incubated with dog serum was increased greatly when the proportion of serum phospholipid to chylomicron phospholipid was increased approximately tenfold (Fig. 1). In the same study, it was demonstrated that phospholipids exchanged between chylomicrons and serum lipoproteins but that the movement from chylomicrons to serum was greater than that in the reverse direction, thus leading to a net loss of phospholipid from the chylomicron fraction. The relation of this transfer to the removal of chylomicrons from the circulation has been discussed by Zilversmit (1969).

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References

  • Abdelkader, A. B., and Mazliak, P., 1969, Echange in vitro de phospholipides entre mitochondries, microsomes et surnageant cytoplasmique de cellules de pomme de terre, C. R. Acad. Sci. Paris 269: 697.

    Google Scholar 

  • Abdelkader, A. B., and Mazliak, P., 1970, Echanges de lipides entre mitochondries, microsomes et surnageant cytoplasmique de cellules de pomme de terre ou de chou-fleur, Ear. J. Biochern. 15: 250.

    Article  CAS  Google Scholar 

  • Akiyama, M., and Sakagami, T., 1969, Exchange of mitochondrial lecithin and cephalin with those in rat liver microsomes, Biochim. Biophys. Acta 187: 105.

    Article  PubMed  CAS  Google Scholar 

  • Bangham, A. D., 1963, Physical structure and behavior of lipids and lipid enzymes, in: Advances in Lipid Research, Vol. I, ( R. Paoletti and D. Kritchevsky, eds.), pp. 65–104, Academic Press, New York.

    Google Scholar 

  • Barsukov, L. I., Shapiro, Yu, E., Viktorov, A. V., Volkova, V. 1., Bystrov, V. F., and Bergelson, L. D., 1974, Study of intervesicular phospholipid exchange by NMR, Biochenr. Biophys. Res. Commun. 60: 196.

    Article  CAS  Google Scholar 

  • Beattie, D. S., 1969, The relationship of protein and lipid synthesis during the biogenesis of mitochondrial membranes, J. Membr. Biol. 1: 383.

    Article  Google Scholar 

  • Berden, J. A., Berker, R. W., and Radda, G. K., 1975, NMR studies on phospholipid bilayers. Some factors affecting lipid distribution, Biochim. Biophys. Acta 375: 186.

    Article  PubMed  CAS  Google Scholar 

  • Blok, M. C., Wirtz, K. W. A., and Scherphof, G. L., 1971, Exchange of phospholipids between microsomes and inner and outer mitochondrial membranes of rat liver, Biochim. Biophys. Acta 233: 61.

    Article  PubMed  CAS  Google Scholar 

  • Bretscher, M. S., 1973, Membrane structure: some general principles, Science 181: 622.

    Article  PubMed  CAS  Google Scholar 

  • Butler, M. M., and Thompson, W., 1975, Transfer of phosphatidylserine from liposomes or microsomes to mitochondria. Stimulation by a cell supernatant factor, Biochim. Biophys. Acta 388: 52.

    Article  PubMed  CAS  Google Scholar 

  • Dawson, R. M. C., 1973, The exchange of phospholipids between cell membranes, Sub-Cell. Biochern. 2: 69.

    CAS  Google Scholar 

  • Dawson, R. M. C., Jungalwala, F. B., Miller, E., and McMurray, W. C., 1972, Synthesis and exchange of phospholipids within brain and liver cells, in: Current Trends in the Biochemistry of Lipids, ( J. Ganguly and R. M. S. Smellie, eds.), pp. 365–376, Academic Press, New York.

    Google Scholar 

  • De Gier, J., Mandersloot, J. G., and Van Deenen, L. L. M., 1968, Lipid composition and permeability of liposomes, Biochim. Biophys. Acta 150: 666.

    Article  PubMed  Google Scholar 

  • Demel, R. A., Van Deenen, L. L. M., and Pethica, B. A., 1967, Monolayer interactions of phospholipids and cholesterol, Biochim. Biophys. Acta 135: 11.

    Article  CAS  Google Scholar 

  • Demel, R. A., Wirtz, K. W. A., Kamp, H. H., Geurts van Kessel, W. S. M., and van Deenen, L. L. M., 1973, Phosphatidylcholine exchange protein from beef liver, Nature (London) New Biol. 246: 102.

    CAS  Google Scholar 

  • Eder, H. A., Bragdon, J. I., and Boyle, E., 1954, The in vitro exchange of phospholipid phosphorus between lipoproteins, Circulation 10: 603.

    Google Scholar 

  • Ehnholm, C., and Zilversmit, D. B., 1972, Use of Forssman antigen in the study of phos- phatidylcholine exchange between liposomes, Biochim. Biophys. Acta 274: 652.

    Article  PubMed  CAS  Google Scholar 

  • Ehnholm, C., and Zilversmit, D. B., 1973, Exchange of various phospholipids and of cholesterol between liposomes in the presence of highly purified phospholipid exchange protein, J. Biol. Chem. 248: 1719.

    PubMed  CAS  Google Scholar 

  • Fielding, C. J., 1972, Further characterisation of lipoprotein lipase and hepatic post-heparin lipase from rat plasma, Biochim. Biophys. Acta 280: 569.

    Article  PubMed  CAS  Google Scholar 

  • Finer, E. G., Flook, A. G., and Hauser, H., 1972, The nature and origin of the NMR spectrum of unsonicated and sonicated aqueous egg yolk lecithin dispersions, Biochim. Biophys. Acta 260: 59.

    Article  PubMed  CAS  Google Scholar 

  • Fleischman, J. B., 1967, Synthesis of the yG heavy chain in rabbit lymph node cells, Biochemistry 6: 1311.

    Article  PubMed  CAS  Google Scholar 

  • Florsheim, W. H., and Morton, M. E., 1957, Stability of phospholipid binding in human serum lipoproteins, J. Appl. Physiol. 10: 301.

    PubMed  CAS  Google Scholar 

  • Gordesky, S. E., and Marinetti, G. V., 1973, The asymmetric arrangement of phospholipids in the human erythrocyte membrane, Biochenr. Biophys. Res. Commun. 50: 1027.

    Article  CAS  Google Scholar 

  • Grant, C. W. M., and McConnell, H. M., 1973, Fusion of phospholipid vesicles with viable Acholeplasma laidlawii, Proc. Natl. Acad. Sci. USA 70: 1238.

    Article  PubMed  CAS  Google Scholar 

  • Green, D. E., Lester, R. L., and Ziegler, D. M., 1957, Studies on the mechanism of oxidative phosphorylation. I. Preparation and properties of a phosphorylating electron transfer particle from beef heart mitochondria, Biochim. Biophys. Acta 23: 516.

    Article  PubMed  CAS  Google Scholar 

  • Harvey, M. S., Wirtz, K. W. A., Kamp, H. H., Zegers, B. J. M., and Van Deenen, L. L. M., 1973, A study on phospholipid exchange proteins present in the soluble fractions of beef liver and brain, Biochim. Biophys. Acta 323: 234.

    Article  PubMed  CAS  Google Scholar 

  • Hauser, H., and Barratt, M. D., 1973, Effect of chain length on the stability of lecithin bilayers, Biochem. Biophys. Res. Commun. 53: 399.

    Article  PubMed  CAS  Google Scholar 

  • Hellings, J. A., Kamp, H. H., Wirtz, K. W. A., and Van Deenen, L. L. M., 1974, Transfer of phosphatidylcholine between liposomes, Eur. J. Biochem. 47: 601.

    Article  PubMed  CAS  Google Scholar 

  • Helmkamp, G. M., Harvey, M. S., Wirtz, K. W. A., and Van Deenen, L. L. M., 1974, Phospholipid exchange between membranes. Purification of bovine brain proteins that preferentially catalyze the transfer of phosphatidylinositol, J. Biol. Chem. 249: 6382.

    PubMed  CAS  Google Scholar 

  • Huang, C.-H., Sipe, J. P., Chow, S. T., and Martin, R. B., 1974, Differential interaction of cholesterol with phosphatidylcholine on the inner and outer surfaces of lipid bilayer vesicles, Proc. Natl. Acad. Sci. USA 71: 359.

    Article  PubMed  CAS  Google Scholar 

  • Illingworth, D. R., and Portman, O. W., 1972, Independence of phospholipid and protein exchange between plasma lipoproteins in vivo and in vitro, Biochim. Biophys. Acta 280: 281.

    Article  PubMed  CAS  Google Scholar 

  • Illingworth, D. R., Portman, O. W., Robertson, A. L., Jr., and Magyar, W. A., 1973, The exchange of phospholipids between plasma lipoproteins and rapidly dividing human cells grown in tissue culture, Biochim. Biophys. Acta 306: 422.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, L. W., and Zilversmit, D. B., 1973, unpublished observations.

    Google Scholar 

  • Johnson, L. W., and Zilversmit, D. B., 1975, Catalytic properties of phospholipid exchange protein from bovine heart, Biochim. Biophys. Acta 375: 165.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, L. W., Hughes, M. E., and Zilversmit, D. B., 1975, Use of phospholipid exchange protein to measure inside—outside transposition in phosphatidylcholine liposomes, Biochim. Biophys. Acta 375: 176.

    Article  PubMed  CAS  Google Scholar 

  • Jungalwala, F. B., Freinkel, N., and Dawson, R. M. C., 1971, The metabolism of phosphatidylinositol in the thyroid gland of the pig, Biochem. J. 123: 19.

    PubMed  CAS  Google Scholar 

  • Kadenbach, B., 1966, Synthesis of mitochondria) proteins: demonstration of a transfer of proteins from microsomes into mitochondria, Biochim. Biophys. Acta 134: 430.

    Google Scholar 

  • Kader, J. C., 1975, Proteins and the intracellular exchange of lipids. I. Stimulation of phospholipid exchange between mitochondria and microsomal fractions by proteins isolated from potato tuber, Biochim. Biophys. Acta 380: 31.

    Article  PubMed  CAS  Google Scholar 

  • Kagawa, Y., Johnson, L.W., and Racker, E., 1973, Activation of phosphorylating vesicles by net transfer of phosphatidyl choline by phospholipid transfer protein, Biochem. Biophys. Res. Commun. 50: 245.

    Article  PubMed  CAS  Google Scholar 

  • Kamath, S. A., and Rubin, E., 1973, The exchange of phospholipids between subcellular organelles of the liver, Arch. Biochem. Biophys. 158: 312.

    Article  PubMed  CAS  Google Scholar 

  • Kamath, S. A., and Rubin, E., 1974, A short procedure to measure phospholipid exchange in mitochondria and microsomes, Anal. Biochem. 59: 172.

    Article  PubMed  CAS  Google Scholar 

  • Kamp, H. H., and Wirtz, K. W. A., 1974, Phosphatidylcholine exchange protein from beef liver, in: Methods in Enzymology, Vol. XXXII, Part B ( S. Fleischer and L. Packer, eds.), pp. 140–146, Academic Press, New York.

    Google Scholar 

  • Kamp, H. H., Wirtz, K. W. A., and Van Deenen, L. L. M., 1973, Some properties of phosphatidylcholine exchange protein purified from beef liver, Biochim. Biophys. Acta 318: 313.

    Article  CAS  Google Scholar 

  • Kornberg, R. D., and McConnell, H. M., 1971a, Lateral diffusion of phospholipids in a vesicle membrane, Proc. Natl. Acad. Sci. USA 68: 2564.

    Article  PubMed  CAS  Google Scholar 

  • Kornberg, R. D., and McConnell, H. M., 1971b, Inside—outside transitions of phospholipids in vesicle membranes, Biochemistry 10: 1111.

    Article  PubMed  CAS  Google Scholar 

  • Kunkel, H. G., and Bearn, A. G., 1954, Phospholipid studies of different serum lipoproteins employing Pia, Proc. Soc. Exp. Biol. Med. 86: 887.

    Article  PubMed  CAS  Google Scholar 

  • Lutton, C., and Zilversmit, D. B., 1976, Lipids 11: 16.

    Article  PubMed  CAS  Google Scholar 

  • McCandless, E. L., and Zilversmit, D. B., 1958, Fate of triglycerides and phospholipids of lymph and artificial fat emulsions: disappearance from the circulation, Am. J. Physiol. 193: 294.

    PubMed  CAS  Google Scholar 

  • McMurray, W. C., and Dawson, R. M. C., 1969, Phospholipid exchange reactions within the liver cell, Biochem. J. 112: 91.

    PubMed  CAS  Google Scholar 

  • McNamee, M. G., and McConnell, H. M., 1973, Transmembrane potentials and phospholipid flip-flop in excitable membrane vesicles, Biochemistry 12: 2951.

    Article  PubMed  CAS  Google Scholar 

  • Michaelson, D. M., Horwitz, A. F., and Klein, M. P., 1973, Transbilayer asymmetry and surface homogeneity of mixed phospholipids in cosonicated vesicles, Biochemistry 12: 2637.

    Article  PubMed  CAS  Google Scholar 

  • Miller, E. K., and Dawson, R. M. C., 1972, Exchange of phospholipids between brain membranes in vitro, Biochem. J. 126: 823.

    PubMed  CAS  Google Scholar 

  • Minari, O., and Zilversmit, D. B., 1963, Behavior of dog lymph chylomicron lipid constituents during incubation with serum, J. Lipid Res. 4: 424.

    PubMed  CAS  Google Scholar 

  • Morgan, R. G. H., and Hofmann, A. F., 1970, Synthesis and metabolism of glycerol-3H triether, a nonabsorbable oil-phase marker for lipid absorption studies, J. Lipid Res. 11: 223.

    PubMed  CAS  Google Scholar 

  • Possmayer, F., 1974, Evidence for a specific phosphatidyl inositol transferring protein in rat brain, Brain Res. 74: 167.

    Article  PubMed  CAS  Google Scholar 

  • Sauner, M.-T., and Lévy, M., 1971, Study of the transfer of phospholipids from the endoplasmic reticulum to the outer and inner mitochondrial membranes, J. Lipid Res. 12: 71.

    PubMed  CAS  Google Scholar 

  • Taniguchi, M., Hirayama, H., and Sakagami, T., 1973, Exchange of molecular species of phosphatidylcholines and phosphatidylethanolamines between rat liver mitochondria and microsomes in vitro, Biochim. Biophys. Acta 296: 65.

    Article  PubMed  CAS  Google Scholar 

  • Tarlov, A., 1968, Turnover of mitochondria] phospholipids by exchange with soluble proteins in vitro, Fed. Proc. 27: 458.

    Google Scholar 

  • Van den Besselaar, A. M. H. P., Helmkamp, G. M., Jr., and Wirtz, K. W. A., 1975, Kinetic model of the protein-mediated phosphatidylcholine exchange between single bilayer liposomes, Biochemistry 14: 1852.

    Article  PubMed  Google Scholar 

  • Wirtz, K. W. A., 1971, Intracellular Transfer of Membrane Phospholipids, Ph.D. Thesis, State University of Utrecht, Utrecht, The Netherlands.

    Google Scholar 

  • Wirtz, K. W. A., 1974, Transfer of phospholipids between membranes, Biochim. Biophys. Acta 344: 95.

    Article  PubMed  CAS  Google Scholar 

  • Wirtz, K. W. A., and Kamp, H. H., 1972, Exchange of phosphatidylcholine between mitochondria and microsomes: stimulation by a specific protein, in: Role of Membranes in Secretory Processes, ( L. Bolis, R. D. Keynes, and W. Wilbrandt, eds.), pp. 52–61, North-Holland, Amsterdam.

    Google Scholar 

  • Wirtz, K. W. A., and Zilversmit, D. B., 1968a, Exchange of phospholipids between sub-cellular particles of liver in vitro, Fed. Proc. 27: 636.

    Google Scholar 

  • Wirtz, K. W. A., and Zilversmit, D. B., 1968b, Exchange of phospholipids between liver mitochondria and microsomes in vitro, J. Biol. Chem. 243: 3596.

    PubMed  CAS  Google Scholar 

  • Wirtz, K. W. A., and Zilversmit, D. B., 1969, Participation of soluble liver proteins in the exchange of membrane phospholipids, Biochim. Biophys. Acta 193: 105.

    Article  PubMed  CAS  Google Scholar 

  • Wirtz, K. W. A., and Zilversmit, D. B., 1970, Partial purification of phospholipid exchange protein from beef heart, FEBS Lett. 7: 44.

    Article  PubMed  CAS  Google Scholar 

  • Wirtz, K. W. A., Van Golde, L. M. G., Van Deenen, L. L. M., 1970, The exchange of molecular species of phosphatidylcholine between mitochondria and microsomes of rat liver, Biochim. Biophys. Acta 218: 176.

    Article  PubMed  CAS  Google Scholar 

  • Wirtz, K. W. A., Kamp, H. H., and Van Deenen, L. L. M., 1972, Isolation of a protein from beef liver which specifically stimulates the exchange of phosphatidylcholine, Biochim. Biophys. Acta 274: 606.

    Article  PubMed  CAS  Google Scholar 

  • Wojtczak, L., Baranska, J., Zborowski, J., and Drahota, Z., 1971, Exchange of phospholipids between microsomes and mitochondrial outer and inner membranes, Biochim. Biophys. Acta 249: 41.

    Article  PubMed  CAS  Google Scholar 

  • Yamakawa, T., Irie, R., and Iwanaga, M., 1960, The chemistry of lipid of posthemolytic residue or stroma of erythrocytes. IX. Silicic acid chromatography of mammalian stroma glycolipids, J. Biochem. 48: 490.

    CAS  Google Scholar 

  • Zborowski, J., and Wojtczak, L., 1975, Net transfer of phosphatidylinositol from microsomes and mitochondria to liposomes catalyzed by the exchange protein from rat liver, FEBS Lett. 51: 317.

    Article  PubMed  CAS  Google Scholar 

  • Zilversmit, D. B., 1969, Chylomicrons, in: Structural and Functional Aspects of Lipoproteins in Living Systems, ( E. Tria and A. M. Scanu, eds.), pp. 329–368, Academic Press, New York.

    Google Scholar 

  • Zilversmit, D. B., 1971a, Stimulation of phospholipid exchange between mitochondria and artificially prepared phospholipid aggregates by a soluble fraction from liver, J. Biol. Chem. 246: 2645.

    PubMed  CAS  Google Scholar 

  • Zilversmit, D. B., 1971b, Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig, J. Lipid Res. 12: 36.

    PubMed  CAS  Google Scholar 

  • Zilversmit, D. B., 1971c, unpublished observations.

    Google Scholar 

  • Zilversmit, D. B., and Hughes, M. E., 1974, unpublished observations.

    Google Scholar 

  • Zilversmit, D. B., and Johnson, L. W., 1975, Purification of phospholipid exchange proteins from beef heart, in: Methods in Enzymology, Vol. 35 ( J. M. Lowenstein, ed.), pp. 262–269, Academic Press, New York.

    Google Scholar 

  • Zwaal, R. F. A., Roelofsen, B., and Colley, C. M., 1973, Localization of red cell membrane constituents, Biochim. Biophys. Acta 300: 159.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Nutritional Sciences and Section of Biochemistry Molecular and Cell Biology, Division of Biological Sciences, Cornell University, Ithaca, NY, 14853, USA

    D. B. Zilversmit (Career Investigator of the American Heart Association) & M. E. Hughes

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  1. D. B. Zilversmit
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  2. M. E. Hughes
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Editors and Affiliations

  1. Laboratory of Cell Biology, National Heart and Lung Institute, Bethesda, Maryland, USA

    Edward D. Korn

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Zilversmit, D.B., Hughes, M.E. (1976). Phospholipid Exchange between Membranes. In: Korn, E.D. (eds) Methods in Membrane Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5820-7_4

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  • DOI: https://doi.org/10.1007/978-1-4757-5820-7_4

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