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Lipid Raft Proteins and Their Identification in T Lymphocytes

  • Chapter
Membrane Dynamics and Domains

Part of the book series: Subcellular Biochemistry ((SCBI,volume 37))

Abstract

This review focuses on how membrane lipid rafts have been detected and isolated, mostly from lymphocytes, and their associated proteins identified. These proteins include transmembrane antigens/receptors, GPI-anchored proteins, cytoskeletal proteins, Src-family protein kinases, G-proteins, and other proteins involved in signal transduction. To further understand the biology of lipid rafts, new methodological approaches are needed to help characterize the raft protein component, and changes that occur in this component as a result of cell perturbation. We describe the application of new proteomic approaches to the identification and quantification of raft proteins in T-lymphocytes. Similar approaches, applied to other model cell systems, will provide valuable new insights into both cellular signal transduction and lipid raft biology.

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Author notes

  1. Priska D. von Haller

    Present address: MacroGenics Inc., 1441 North 34th Street, Seattle, WA, 98103, USA

  2. David R. Goodlett

    Present address: Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, WA, 98195, USA

Authors and Affiliations

  1. Institute for Systems Biology, 1441 North 34th Street, Seattle, WA, 98103, USA

    Bernd Wollscheid, Priska D. von Haller, Eugene Yi, Samuel Donohoe, Kelly Vaughn, Andrew Keller, Alexey I. Nesvizhskii, Jimmy Eng, Xiao-jun Li, David R. Goodlett, Ruedi Aebersold & Julian D. Watts

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    Peter J. Quinn

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Wollscheid, B. et al. (2004). Lipid Raft Proteins and Their Identification in T Lymphocytes. In: Quinn, P.J. (eds) Membrane Dynamics and Domains. Subcellular Biochemistry, vol 37. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5806-1_3

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