Abstract
After its development by Levin and Bang in 1968 (4), the limulus amebocyte lysate test had been considered specific for endotoxin until Kakinuma et al., found that carboxymethylated (1→3) −β -D-glucan also activates limulus coagulation enzymes (3). Morita et al., showed that amebocyte lysate contains, in addition to endotoxin-sensitive factor C, a factor G that is sensitive for a trace amount of (1→3)− β -D-glucan (Fig 1a) (5).
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References
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Iwanaga, S., Morita, T., Miyata, T.. et al., 1984, The limulus coagulation system sensitive to bacterial endotoxins. in: “Bacterial endotoxin: chemical, biological, and clinical aspects.” J. Y. Homma et al., eds. Weinheim: Verlag Chemie GmbH, 365–382.
Kakinuma, A., Asano, T., Torii, H., and Sugino, Y., 1981. Gelation of Limulus amebocyte lysate by an antitumor (1-3) -13 -D-glucan. Biochem. Biophys. Res. Commun. 101: 434–439.
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Obayashi, T., 1984, Addition of perchloric acid to blood samples for colorimetric limulus test using chromogenic substrate: comparison with conventional procedures and clinical applications. J. Lab. Clin. Med. 104: 321–330.
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© 1990 Springer Science+Business Media New York
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Obayashi, T. (1990). A New Endotoxin-Specific Assay. In: Friedman, H., Klein, T.W., Nakano, M., Nowotny, A. (eds) Endotoxin. Advances in Experimental Medicine and Biology, vol 256. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-5140-6_19
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DOI: https://doi.org/10.1007/978-1-4757-5140-6_19
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