Abstract
Following coronary artery ligation (CAL), levels of cAMP and the activity ratio of cAMP-dependent protein kinase, of Phosphorylase kinase, and of Phosphorylase are significantly elevated in both ischemic and nonischemic areas of the canine left ventricle. The aerobic level of cAMP was found to be 0.4 to 0.6 pmol/mg myocardium only after a precooled clamp or a cryobiopsy device was employed to guarantee tissue freezing in situ. Maximal changes in response to ischemia are observed within 2 min in both parts of the heart. Twenty minutes after the onset of ischemia, different responses have been found in the nonischemic and ischemic tissue. Whereas the levels of cAMP and the activity ratio of protein kinase, of Phosphorylase kinase, and of Phosphorylase returned to aerobic values in the nonischemic area, these parameters remained elevated in the ischemic area. The changes in the levels of myocardial cAMP and in the cAMP-dependent protein kinase activity ratio following CAL could be prevented by propranolol.
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References
Cohen, P. 1973. The subunit structure of rabbit skeletal muscle Phosphorylase kinase and the molecular basis of its activation reactions. Eur. J. Biochem. 34:1–14.
Corr, P. B., Witkowski, F. X., and Sobel, B. E. 1978. Mechanism contributing to malignant dysrhythmias induced by ischemia in the cat. J. Clin. Invest. 61:109–119.
Dobson, J. G. 1978. Protein kinase regulation of cardiac Phosphorylase activity and contractility. Am. J. Physiol. 234:H638-H648.
Dobson, J. G., and Mayer, S. E. 1973. Mechanisms of activation of cardiac glycogen Phosphorylase in ischemia and anoxia. Circ. Res. 33:412–420.
Gilman, A. 1970. A protein binding assay for adenosine 3′,5′-cyclic monophosphate. Proc. Natl. Acad. Sci. U.S.A. 67:305–312.
Harper, J. R. and Brooker, G. 1975. Femtomole sensitive radioimmunoassay for cyclic AMP and cyclic GMP after 2’0 acetylation by acetic anhydride in aqueous solution. J. Cyclic Nucleotide Res. 1:207–218.
Illingworth, B., and Cori, G. T. 1953. Crystalline muscle Phosphorylase. Biochem. Prep. 3:1–9.
Keely, S. L., and Corbin, J. D. 1977. Involvement of cAMP-dependent protein kinase in the regulation of heart contractile force. Am. J. Physiol. 2:H269-H275.
Krause, E.-G., Bartel, S., Reich, J.-G., and Winkler, J. 1981. On the activation of protein kinase by cAMP in the myocardium in vivo. Biochem. S oc. Trans. 9:24 IP.
Krause, E.-G., and Hosenfelder, W. 1981. Probennehmer zur tiefgekühlten Gewebsentnahme aus biologischen Material. Patentschrift 149464, Amt für Erfindungs- und Patentwesen der DDR, Berlin.
Krause, E.-G. and Wollenberger, A. 1967. Aktivierung der Phosphorylase-6-kinase im akut ischämischen Myokard. Acta Biol. Med. Germ. 19:381–386.
Krause, E.-G., and Wollenberger, A. 1980. Cyclic nucleotides in heart in acute myocardial ischemia and hypoxia. Adv. Cyclic Nucleotide Res. 12:51–61.
Krause, E.-G., Ziegelhöffer, A., Fedelesova, M., Styk, J., Kostolansky, S., Gabauer, I., Blasig, I., and Wollenberger, A. 1978. Myocardial cyclic nucleotide levels following coronary artery ligation. Adv. Cardiol. 25:119–129.
Krebs, E. G., Graves, J. G., and Fischer, E. H. 1959. Factors affecting the activity of muscle Phosphorylase b kinase. J. Biol. Chem. 234:2867–2873.
Malliani, A., Schwartz, P. J., and Zanchetti, A. 1969. A sympathetic reflex elicited by experimental coronary occlusion. Am. J. Physiol. 217:703–709.
Matsushita, S., Shinawaga, T., Sukai, M., Moroki, N., Karamato, K., and Murakami, M. 1978. Comparison of adenosine 3′:5′ monophosphate dependent protein kinase from various cardiac muscle. In: VIII. World Congress on Cardiology, Tokyo, p. 560.
Nimmo, M. G., and Cohen, P. 1977. Hormonal control of protein phosphorylation. Adv. Cyclic Nucleotide Res. 8:146–266.
Opie, L. H., Nathan, D., and Lubbe, W. F. 1979. Biochemical aspects of arrhythmogenesis and ventricular fibrillation. Am. J. Cardiol. 43:131–148.
Shahab, L., Haase, M., Schiller, U., and Wollenberger, A. 1969. Noradrenalinabgabe aus dem Hundeherzen nach vorübergehender Okklusion einer Koronararterie. Acta Biol. Med. Germ. 22:135–143.
Tsien, R. W. 1977. Cyclic AMP and contractile activity in heart. Adv. Cyclic Nucleotide Res. 8:364–420.
Podzuweit, T., Dalby, A. J., Cherry, G. W., and Opie, L. H. 1978. Cyclic AMP levels in ischemic and nonischemic myocardium following coronary artery ligation: Relation to ventricular fibrillation. J. Mol. Cell. Cardiol. 10:81–94.
Pool, P. E., Norris, G. F., Levis, R. M., and Covell, J. W. 1968. A biopsy-drill permitting rapid freezing. J. Appl. Physiol. 24:832–833.
Wollenberger, A. 1975. The role of cyclic AMP in the adrenergic control of myocardium. In: W. G. Nayler (ed.), Contraction and Relaxation of the Heart, pp. 113–190. Academic Press, London.
Wollenberger, A., Krause, E.-G., and Heier, G. 1969. Stimulation of 3’,5’-cyclic AMP formation in dog myocardium following arrest of blood flow. Biochem. Biophys. Res. Commun. 36:664–670.
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Krause, EG., Bartel, S., Karczewski, P., Lindenau, KF. (1983). Cyclic Nucleotides and Changes in Protein Kinase Activity Ratio in the Ischemic and Nonischemic Myocardium. In: Chazov, E., Saks, V., Rona, G. (eds) Advances in Myocardiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-4441-5_50
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DOI: https://doi.org/10.1007/978-1-4757-4441-5_50
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