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Fluorescence Sensing

  • Chapter
Principles of Fluorescence Spectroscopy

Abstract

Fluorescence sensing of chemical and biochemical analytes is an active area of research.1–7 This research is being driven by the desire to eliminate radioactive tracers, which are costly to use and dispose of. Additionally, there is a need for rapid and low-cost testing methods for a wide range of clinical, bioprocess, and environmental applications. During the past decade, we have witnessed the introduction of numerous methods based on high-sensitivity fluorescence detection, including DNA sequencing, DNA fragment analysis, fluorescence staining of gels following electrophoretic separation, and a variety of fluorescence immunoassays. Historically, one can trace many of these analytical applications to the classic reports by Undenfriend and co-workers,8,9 which anticipated many of today’s applications of fluorescence. More recent monographs have summarized the numerous analytical applications of fluorescence.10–14

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    Joseph R. Lakowicz

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Lakowicz, J.R. (1999). Fluorescence Sensing. In: Principles of Fluorescence Spectroscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-3061-6_19

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