Conjugation in Escherichia coli

Abstract

The discovery of the process of conjugation in prokaryotes was due to one of the most fortuitous experimental designs in recent scientific history. Many other scientists had tried to demonstrate conjugation and had failed. Nevertheless, Lederberg and Tatum, working at Yale University, chose to make another attempt using a common laboratory strain of bacteria, Escherichia coli K-12, as their experimental system. They also decided to test only a few isolates of the strain. By chance, they chose not only one of the few organisms that readily undergoes conjugation but also a fertile stain of that organism. In so doing they started an extremely fruitful branch of bacterial genetic research. E. coli remains the standard conjugation system to which all others are initially compared. In this chapter the E. coli system is presented. Several alternative conjugation systems are discussed in Chapter 10.