Abstract
Colony Stimulating Factor-1 (CSF-1) is required for the in vitro survival, proliferation and differentiation of mononuclear phagocytes (precursor cell → monoblast → promonocyte → monocyte → macrophage) (reviewed in 1). Purified murine or human CSF-1 has been shown to be a hanodimer consisting of two identical ~ 14,000 Mr polypeptide chains that are maintained as a differ by disulfide bonds. It is heavily glycosylated with N-linked oligosaccharides of the acidic complex type. The variable molecular weight (Mr) of the native molecule within the range of 45,000–70,000 is entirely ascribable to variation in glycosylation. While the carbohydrate does not appear to be necessary for biological, antibody-binding, or receptor-binding activities, all of these activities are lost when the molecule is gently reduced to yield monomeric subunits2.
Keywords
- Protein Phosphorylation
- Mononuclear Phagocyte
- Macrophage Cell Line
- Albert Einstein College
- Variable Molecular Weight
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Jubinsky, P.T., Yeung, YG., Sacca, R., Li, W., Stanley, E.R. (1988). Colony Stimulating Factor-1 Stimulated Macrophage Membrane Protein Phosphorylation. In: Kudlow, J.E., MacLennan, D.H., Bernstein, A., Gotlieb, A.I. (eds) Biology of Growth Factors. Advances in Experimental Medicine and Biology, vol 234. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1980-2_7
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