Abstract
The specificity with which antibodies react with their target forms the basis of a number of analytical methods that can be used to confirm the identity of a protein, and to screen samples (e.g., column fractions) for the presence of a specific protein. Two of the most common immunochemical methods for protein analysis are Western blots and antibody-capture assays. These shall be described in detail in this chapter. Descriptions of other immuno-chemical methods, such as rocket electrophoresis and immunoprecipitation can be found in several texts that are devoted exclusively to immuno-chemistry (Axelsen, 1975; Bjerrum and Heegaard, 1988; Harlow and Lane, 1988). Before describing these analytical methods, a brief overview of immunology is presented, so that the terms used later in this chapter are clearly defined. A more thorough discussion of antibodies and immunology can be found in the text by Harlow and Lane (1988).
Keywords
- Antibody Capture
- Rhodobacter Sphaeroides
- Immunological Method
- Immunochemical Method
- Paracoccus Denitrificans
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Andrews, A. T. (1986) Electrophoresis: Theory, Techniques and Biochemical and Clinical Applications, 2d ed., Oxford University Press, Oxford.
Axelsen, N. D. (1975) Quantitative Immunoelectrophoresis: Methods and Applications, Blackwell Scientific, Oxford.
Bjerrum, O. J., and Heegaard, N. H. H. (1988) Handbook of Immunoblotting of Proteins, CRC Press, Boca Raton, FL.
Bollag, D. M., and Edelstein, S. J. (1991) Protein Methods, Wiley, New York.
Harlow, E., and Lane, D. (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Towbin, H.; Staehelin, T.; and Gordon, J. (1979) Proc. Natl. Acad. Sci. USA, 76, 4350–4354.
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© 1994 Springer Science+Business Media Dordrecht
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Copeland, R.A. (1994). Immunological Methods. In: Methods for Protein Analysis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-1505-7_5
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DOI: https://doi.org/10.1007/978-1-4757-1505-7_5
Publisher Name: Springer, Boston, MA
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