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Development of a Micro HG-PRT Activity Assay: Preliminary Complementation Studies with Lesch — Nyhan Cell Strains

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Purine Metabolism in Man

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 41))

Abstract

The main purpose of the present study was to look with the heteropolykaryontest (ref. 1,2) for genetic complementation between different HG-PRT deficient cell strains, which had been fused and cultured in Plastic Film Dishes (PFD’s ref, 3,4) A radiochemical HG-PRT microassay, based on the use of the Parafilm Micro Cuvette (PMC; ref. 3,4) was developed, which permits the quantification of the enzyme activity at the single cell level.

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References

  1. P. Hösli: (1972) Microtechniques for the detection of intergenic and interallelic complementation in man. Bull. Europ. Soc. Hum. Genet, p.32.

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  2. P. Hösli: (1973) Inborn lysosomal disease: detection of genetic heterogeneity by complementation studies. Proceedings of the “Third International Research Conference on Lysosomes in Cell Pathology”, Louvain, in press.

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  3. P. Hösli: (1972) Tissue cultivation on plastic films. Tecnomara A.G., Rieterstrasse 59, Postfach CH 8059, Zurich, Zwitserland.

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  4. P. Hösli: (1972) Micro techniques for rapid prenatal diagnosis of inborn errors of metabolism in early pregnancy. Prenatal diagnosis newsletter 1 (1972) 10.

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Author information

Authors and Affiliations

  1. Department of Human Genetics, Sarphatistraat 217, Amsterdam, The Netherlands

    P. Hösli

  2. Department of Human Genetics, Faculty of Medicine, University of Nijmegen, Nijmegen, The Netherlands

    C. H. M. M. de Bruyn & T. L. Oei

Authors
  1. P. Hösli
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  2. C. H. M. M. de Bruyn
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  3. T. L. Oei
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Editor information

Editors and Affiliations

  1. Division of Metabolic Disease, Rogoff-Wellcome Medical Research Institute, Beilinson Hospital, Petah-Tikva, Israel

    Oded Sperling  & Andre De Vries  & 

  2. Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA

    James B. Wyngaarden

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© 1974 Springer Science+Business Media New York

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Hösli, P., de Bruyn, C.H.M.M., Oei, T.L. (1974). Development of a Micro HG-PRT Activity Assay: Preliminary Complementation Studies with Lesch — Nyhan Cell Strains. In: Sperling, O., De Vries, A., Wyngaarden, J.B. (eds) Purine Metabolism in Man. Advances in Experimental Medicine and Biology, vol 41. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-1433-3_60

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  • DOI: https://doi.org/10.1007/978-1-4757-1433-3_60

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4757-1435-7

  • Online ISBN: 978-1-4757-1433-3

  • eBook Packages: Springer Book Archive

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