Abstract
A method was developed for the rapid isolation and identification of kallidin produced by hog pancreatic kallikrein on a partially purified bovine plasma kininogen preparation. By means of a reversed phase chromatography on a C18-silica gel column, kallidin was rapidly concentrated from the reaction mixture, and subsequently resolved on a thin-layer plate of silica gel 60. Fluorescamine was used to locate the kinin. Efforts to elute the kinin from the TLC plates for quantitation were not successful.
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© 1979 Springer Science+Business Media New York
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Khouw, B.T. (1979). An Approach to the Chemical Quantitation of Kinins for the Assay of Kininogenases. In: Fujii, S., Moriya, H., Suzuki, T. (eds) Kinins—II. Advances in Experimental Medicine and Biology, vol 120. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0926-1_7
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DOI: https://doi.org/10.1007/978-1-4757-0926-1_7
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