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Biological Reactive Intermediates—II pp 619–631Cite as

Destruction of Microsomal Calcium Pump Activity: A Possible Secondary Mechanism in BrCCl3 and CCl4 Liver Cell Injury

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 136))

Abstract

In vitro rat liver microsomes free of Fe2+ ions peroxidize minimally at 37° when NADPH and either CC14 or BrCC13 are added. Although the lipid peroxidation dependent on toxigenic haloalkanes in these Fe2+-free microsome systems is very low, it is considerably more efficient in causing loss of cytochrome P-450 and glucose6-phosphatase than is the far more vigorous lipid peroxidation dependent on presence of Fe2+ ions. In particular, the Ca2+-pump activity of isolated microsomes was almost completely destroyed when malonic dialdehyde (MDA) production was as little as 8 pg per gram equivalent microsomes. Moore et al. (1976) had shown previously that the capacity of liver microsomes to sequester Ca2+ was severely depressed 30 min after CC14 administration to rats. We have shown that this effect is already manifested within 3 min after CC14 administration, by which time peroxidative decomposition of microsomal lipids can be detected. The time course of the destruction of the liver microsomal Ca2+ pump after CC14 administration to rats was essentially identical to the time course of microsomal lipid peroxidation, as revealed by the appearance of conjugated diene configurations in microsomal lipids.

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Authors and Affiliations

  1. Department of PhysiologySchool of Medicine, Case Western Reserve University, Cleveland, Ohio, 44106, USA

    Richard O. Recknagel, Karen Lowrey & Robert L. Waller

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  1. Richard O. Recknagel
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  2. Karen Lowrey
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  3. Robert L. Waller
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  4. Eric A. Glende Jr.
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Editor information

Editors and Affiliations

  1. Rutgers University, Piscataway, New Jersey, USA

    Robert Snyder , Charlotte M. Witmer  & Beatrice N. Engelsberg ,  & 

  2. Medical University of South Carolina, Charleston, South Carolina, USA

    David J. Jollow  & Stephen L. Longacre  & 

  3. University of Surrey, Guildford, Surrey, England

    Dennis V. Parke  & C. Gordon Gibson  & 

  4. Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    James J. Kocsis  & George F. Kalf  & 

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© 1982 Springer Science+Business Media New York

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Recknagel, R.O., Lowrey, K., Waller, R.L., Glende, E.A. (1982). Destruction of Microsomal Calcium Pump Activity: A Possible Secondary Mechanism in BrCCl3 and CCl4 Liver Cell Injury. In: Snyder, R., et al. Biological Reactive Intermediates—II. Advances in Experimental Medicine and Biology, vol 136. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0674-1_45

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  • DOI: https://doi.org/10.1007/978-1-4757-0674-1_45

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