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Eliminating All Nonepithelial Cells Using Colchicine

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Hydra: Research Methods

Abstract

To eliminate the interstitial cell population of hydra using colchicine treatments (Campbell, 1976; Marcum and Campbell, 1978). The resulting viable animals consist only of two layers of epithelial cells plus the noncellular mesoglea. These animals are termed epithelial hydra.

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References

  • Campbell, R. D. 1976. Eliminatio of Hydra interstitial and nerve cells by means of colchicine. J. Cell Sci. z/:1—B.

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  • David, C. N. 1973. A quantitative method for niaceration of hydra tissue, Wilhelm Roux.4 reh. axwicúmngsmoacnrg. 171u59–268.

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  • Marcum, B. A., and Campbell, R. D. 1978. Development of hydra lacking nerve and interstitial

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© 1983 Springer Science+Business Media New York

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Marcum, B.A., Campbell, R.D. (1983). Eliminating All Nonepithelial Cells Using Colchicine. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_37

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  • DOI: https://doi.org/10.1007/978-1-4757-0596-6_37

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0598-0

  • Online ISBN: 978-1-4757-0596-6

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