Abstract
To produce chimeric hydra consisting of ectoderm from one strain and endoderm from a different strain. The procedure involves vital staining, grafting, and microsurgery. Nitrogen mustard-treated host animals are used to support the growth of small chimeras.
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References
Campbell, R. D. 1974. Cell movements in Hydra. Am, Zool, 14:523–535
Macklin, M. 1976. The effect of urethan on hydra. Biol. Bull. 150:442–452
Wanek, N. and Campbell, R D. 1982. Roles of ectodermal and endodermal epithelial cells in hydra rnorphogenesis: construction of chimeric strains.J. Exp. Zool. 221:37–47.
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© 1983 Springer Science+Business Media New York
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Wanek, N. (1983). Preparing Ectoderm/Endoderm Chimeras. In: Lenhoff, H.M. (eds) Hydra: Research Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0596-6_36
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DOI: https://doi.org/10.1007/978-1-4757-0596-6_36
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-0598-0
Online ISBN: 978-1-4757-0596-6
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