Abstract
Several natural variants of mouse hepatitis viruses have been compared by the T1-oligonucleotide fingerprinting technique. In general, they have aiverged quite extensively. However, MHV-3, a hepatotropic strain, and A59, a nonpathogenic strain, were found to be extremely related. Yet, each of them contains 2–4 specific oligonucleotides. One of the MHV-3-specific oligonucleotides was mapped in 30S poly(A)-containing RNA or 6–7 Kb from the 3-end and the other near the 5′-end of the genome. These two genetic regions might be associated with viral pathogenicity. In addition, two JHM plaque variants, DL producing large plaques and DS producing small plaques, were also compared. They share almost all T1-oligonucleotides, but each contains one unique spot. The DL-specific oligonucleotide was mapped in 21S poly(A)-containing RNA or at about 4 kb from the 3′-end. Finally, the MHV genome was found to contain the “cap” structure, confirming that it is a positive-stranded RNA.
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Bailey, O.T., Pappenheimer, A.M., Cheever, F.S., and J.B. Daniels. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. J. Exp. Med. 90: 195–212.
Calisher, C.H. and W.P. Rowe. 1966. Mouse hepatitis, Reo-3 and Theiler viruses. Nat. Cancer Inst. Monogr. 20: 67–75.
Cheever, L.S., Daniels, J.B., Pappenheimer, A.M., Bailey, O.T. 1949. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. I. Isolation and biological properties of the virus. J. Exp. Med. 90: 181–194.
De Wachter, R. and W. Fiers. 1972. Preparative two dimensional polyacrylamide gel electrophoresis of P-labeled RNA. Anal. Biochem. 49: 184–197.
Dick, G.W., Niven, J. and A. Gledhill. 1956. A virus related to that causing hepatitis in mice (MHV). Brit. J. Exp. Path. 38: 90–96.
Furuich, Y. and K. Miura. 1975. A blocked structure at the 5′ terminus of mRNA from cytoplasmic polyhedrosis virus. Nature 253: 374–375.
Gledhill, A. and O.C. Andrews. 1951. A hepatitis virus of mice. Brit. J. Exp. Path. 32: 559–568.
Gledhill, A.W. and J.S.F. Niven. 1955. Latent virus as exemplified by mouse hepatitis virus (MHV). Vet. Rev. Annot. 1: 82–90.
Herndon, R.M., Griffin, D.E., McCormick, U., and Weiner, L.P. 1975. Mouse hepatitis virus-induced recurrent demyelination. Arch. Neurol. 32: 32–35.
Hierholzer, J.C., Broderson, J.R. and F. Murphy. 1979. New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Inf. Immun. 24: 508–522.
Keith, J. and H. Fraenkel-Conrat. 1975. Identification of the 5′-end of Rous sarcoma virus RNA. Proc. Natl. Acad. Sci. USA 72: 3347–3358.
Lai, M.M.C. and Stohlman, S.A. 1978. The RNA of mouse hepatitis virus. J. Virol. 26: 236–242.
Manaker, R.A., Piczak, C., Miller, A. and M. Stanton. 1961. A hepatitis virus complicating studies with mouse leukemia. J. Natl. Canc. Inst. 27: 29–51.
McIntosh, K. 1973. Coronaviruses: A comparative review. Curr. Top. Microbiol. Immunol. 63: 85–129.
Nelson, J.B. 1952. Acute hepatitis associated with mouse leukemia. I. Pathologic features and transmission of the disease. J. Exp. Med. 196: 293–300.
Robb, J.A. and C.W. Bond. 1979. Pathogenic murine coronaviruses. I. Characterization of the biological behavior in vitro and virus-specific intracellular RNA of strongly neurotropic JHMV and weakly neurotropic A59V viruses. Virology 94: 352–370.
Rowe, W., Hartley, J. and W. Capps. 1963. Mouse hepatitis virus infection as a highly contagious, prevalent enteric infection of mice. Proc. Soc. Exp. Biol. Med. 112: 161–165.
Shatkin, A.J. 1974. Methylated messenger RNA synthesis in vitro by purified reovious. Proc. Natl. Acad. Sci. USA 71: 3204–3207.
Stern, D.F. and S.I.T. Kennedy. 1980. Coronavirus multiplication strategy. I. Identification and characterization of virus-specific RNA. J. Virol. 34: 665–674.
Stohlman, S.A. and M.M.C. Lai. 1979. Phosphoproteins of murine coronaviruses. J. Virol. 32: 672–675.
Virelizier, J.L., Dayan, A.D. and Allison, A.C. 1975. Neuro-pathological effects of persistent infection of mice by mouse hepatitis virus (MHV-3). Infection and Immunity 12: 1127–1140.
Wang, L.H., P.H. Duesberg, T. Robins, et al. 1977. The terminal oligonucleotides of avian tumor virus RNAs are genetically linked. Virology 82: 472–492.
Wege, H., A. Muller, and V. ter Meulen. 1978. Genomic RNA of the murine coronavirus of JHM. J. Gen. Virol. 41: 217–228.
Weiner, L.P.: Pathogenesis of demyelination induced by a mouse hepatitis virus (JHM virus). Arch. Neurol. 28: 298–303.
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Lai, M.M.C., Stohlman, S.A. (1981). Genome Structure of Mouse Hepatitis Virus: Comparative Analysis by Oligonucleotide Mapping. In: ter Meulen, V., Siddell, S., Wege, H. (eds) Biochemistry and Biology of Coronaviruses. Advances in Experimental Medicine and Biology, vol 142. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0456-3_5
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DOI: https://doi.org/10.1007/978-1-4757-0456-3_5
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