Abstract
The genome of the murine Coronavirus MHV-JHM is a positive-stranded RNA of approximately 30 Kb, which encodes 4 structural proteins (N, M, S and HE) and at least 4 non-structural proteins. The location of the major open reading frames (ORFs) in the genome is shown in Fig. 1. In the infected cell, expression of the viral proteins is mediated by a set of subgenomic mRNAs. In relation to the genome the mRNAs are 3′ coterminal and extend to different positions in a 5′ direction. The mRNAs 7, 6, 3 and 2-1 have been shown to encode the N, M, S and HE proteins respectively (Siddell, 1983; Pfleiderer et al., this volume). mRNA 4 encodes a 15,000 molecular weight (mol.wt.) non-structural protein (Ebner et al, 1988) and mRNA 5 has the potential to encode two non-structural proteins of 12,400 and 10,200 mol.wt. (Skinner et al., 1985). Earlier in vitro translation studies (Siddell, 1983), indicated that the MHV-JHM subgenomic mRNA 2 encodes a 30,000 mol.wt. nonstructural protein, the expression of which was translationally regulated. Recent sequence analysis of the 5′ proximal region of the closely related MHV-A5 9 mRNA 2 confirms the presence of a 261 aminoacid ORF at this position. Analysis of the predicted polypeptide sequence indicates a non-membrane protein which may possibly have nucleotide binding and phosphorylating properties (Luytjes et al., 1988).
Chapter PDF
Similar content being viewed by others
Keywords
- Fusion Protein
- Nonstructural Protein
- Rabbit Reticulocyte Lysate
- Infected Cell Lysate
- Positive Immune Reaction
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Ebner, D., Raabe, T., and Siddell, S.G., 1988, Identification of the Coronavirus MHV-JHM mRNA4 product. J. gen. Virol. 69: 1041.
Luytjes, W., Bredenbeek, P.J., Noten, A.F.H., Horzinek, M.C., and Spaan, W.J.M., 1988, Virology 166:415.
Siddell, S.G., Wege, H., Barthel, A., and ter Meulen, V., 1981, Coronavirus JHM. Intracellular protein synthesis. J. gen. Viro- 1. 53:145.
Siddell, S.G., 1983, Coronavirus JHM. Coding assignment of subgenomic mRNAs. J. gen. Virol. 64:113.
Skinner, M.A., Ebner, D., and Siddell, S.G., 1985, Coronavirus MHV-JHM mRNA5 has a sequence arrangement which potentially allows translation of a second, downstream open reading frame. J. gen. Virol. 66:581.
Stanley, K.K., and Luzio J.P., 1984, Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins, EMBO J.3:1429.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this paper
Cite this paper
Schwarz, B., Routledge, E., Siddell, S. (1990). Characterization of the MHV-JHM Non-Structural Protein Encoded by mRNA 2. In: Cavanagh, D., Brown, T.D.K. (eds) Coronaviruses and their Diseases. Advances in Experimental Medicine and Biology, vol 276. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5823-7_43
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5823-7_43
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5825-1
Online ISBN: 978-1-4684-5823-7
eBook Packages: Springer Book Archive