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The Usefulness of Studies In Vitro for Understanding Cerebral Metabolite Transport In Vivo

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Transport Phenomena in the Nervous System

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 69))

Abstract

Arguments between proponents of experimentation in vivo and those who favor studying systems in vitro are quite old. The former say that complex living systems cannot be studied in vitro because the changes in these preparations cause observations of artifacts, while the latter say that complex systems have first to be broken into simpler ones before we can hope for meaningful interpretations of our observations. We belong to what we perceive to be the majority, who feel that both approaches are very useful and necessary for studies of nervous system function, and are aware of the need to know the advantages and limitations of both. Similar discussions can still be heard among researchers studying neural barriers: “the blood-brain barrier (and other brain barriers) can be studied only in living animals; perhaps the biggest change in any system in vitro is the absence of the barrier)”, is the statement often heard. Clearly, if we want to study the initial rate of transport from the circulating plasma into brain, studies in vivo are the most suitable; but if we want to study the factors that determine the equilibrium and influence flux, cellular transport processes (requiring observations in vitro) should not be neglected. Hence this chapter.

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Authors and Affiliations

  1. New York State Research Institute for Neurochemistry and Drug Addiction, 10035, Ward’s Island, New York, NY, USA

    Abel Lajtha & Miriam Banay-Schwartz

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  1. Abel Lajtha
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  2. Miriam Banay-Schwartz
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Editors and Affiliations

  1. National Research Council, Rome, Italy

    Giulio Levi

  2. University of Padua Medical School, Padua, Italy

    Leontino Battistin

  3. Research Institute of Neurochemistry, New York, New York, USA

    Abel Lajtha

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© 1976 Plenum Press, New York

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Lajtha, A., Banay-Schwartz, M. (1976). The Usefulness of Studies In Vitro for Understanding Cerebral Metabolite Transport In Vivo . In: Levi, G., Battistin, L., Lajtha, A. (eds) Transport Phenomena in the Nervous System. Advances in Experimental Medicine and Biology, vol 69. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-3264-0_31

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  • DOI: https://doi.org/10.1007/978-1-4684-3264-0_31

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