Abstract
The murine hepatitis virus (MHV) genome is a large single-stranded RNA which encodes the major structural proteins of the virion, the nucleocapsid (N), the membrane (M) and the spike (S) proteins. Additionally, an unknown number of proteins which are either minor virion components, or non-structural proteins, are expressed during infection (Siddell, 1987). The expression of the MHV genome is mediated in the infected cell via a 3′ coterminal nested set of subgenomic mRNAs, which are numbered 1 to 7, in order of decreasing size. The available data (reviewed in Siddell, 1987) suggests that, at least for the structural protein mRNAs (i. e. mRNAs 3, 6 and 7) only the 5′ unique regions i. e. those absent from the next smallest mRNA, are translationally active and only single polypeptides are translated from these regions. In contrast, the unique region of mRNA 5 has two potential open reading frames (ORFs, Skinner et al., 1985) and although the translation products have not yet been identified, this unusual organization suggests that this mRNA may be functionally bicistronic.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
M. Kozak. Comparison of initiation of protein synthesis in pro-caryotes, eucaryotes and organelles. Microbiol Reviews 47: 1 (1983).
P. T. Massa, R. Dörries, and V. ter Meulen. Viral particles induce Ia antigen expression on astrocytes. Nature 320: 543 (1986).
U. Rüther, and B. Müller-Hill. Easy identification of cDNA clones. EMBO J. 2: 1791 (1983).
I. Schmidt, M. Skinner, and S. Siddell. Nucleotide sequence of the gene encoding the surface projection glycoprotein of coronavirus MHV-JHM. J gen Virol 68: (1987) in press.
S. Siddell, H. Wege, A. Barthel, and V. ter Meulen. Coronavirus JHM: Intracellular protein synthesis. J gen Virol 53: 145 (1981)
S. Siddell. Coronavirus JHM: Coding assignments of subgenomic mRNAs. J gen Virol 64: 113 (1983).
S. G. Siddell. Genomic organization of murine coronavirus MHV. “Positive Strand RNA Viruses” UCLA Symposia on Molecular and Cellular Biology, New Series, Volume 54, Editors, M.A. Brinton and R. Rueckert, Alan R. Liss, Inc., New York (1986).
M. A. Skinner, and S.G. Siddell. Coding sequence of coronavirus MHV-JHM mRNA 4. J gen Virol 66: 593 (1985).
M. A. Skinner, D. Ebner, and S.G. Siddell. Coronavirus MHV-JHM mRNA 5 has a sequence arrangement which potentially allows translation of a second, downstream open reading frame. J gen Virol 66: 581 (1985).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Ebner, D., Siddell, S. (1987). Identification of the Coronavirus MHV-JHM mRNA 4 Gene Product Using Fusion Protein Antisera. In: Lai, M.M.C., Stohlman, S.A. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 218. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1280-2_5
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1280-2_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1282-6
Online ISBN: 978-1-4684-1280-2
eBook Packages: Springer Book Archive